The antispasmodic activity of ethanol extract of the stem bark of Piliostigma reticulatum Horscht D.C (Ceasalpiniaceae), and its dichloromethane fraction isolated

Kassim Dosso; Abiba Ouattara Gboko; Dago Serge David Attemene; Banga Benoit N’guessan; Angoue Paul Yapo

doi:10.30574/gscbps.2020.11.1.0049

Authors

Kassim Dosso Laboratory of Physiology and Pharmacology, UFR Environment, University of Jean Lorougnon Guédé of Daloa, P.O 02 Box 150 Daloa 02, Daloa, Côte d'Ivoire.
Abiba Ouattara Gboko Laboratory of Biotechnology in agro-resources valorization and natural substances, UFR Biological Sciences, University of Korhogo, P.O Box 1328 Korhogo, Korhogo, Côte d'Ivoire.
Dago Serge David Attemene Laboratory of Physiology and Pharmacology, UFR Environment, University of Jean Lorougnon Guédé of Daloa, P.O 02 Box 150 Daloa 02, Daloa, Côte d'Ivoire.
Banga Benoit N’guessan Department of Pharmacology and Toxicology, School of Pharmacy, College of Health Sciences, University of Ghana, P.O Box KB 52, Korle-Bu, Accra, Ghana.
Angoue Paul Yapo Laboratory of Physiology and Pharmacology, UFR Natural Sciences, University of Nangui Abrogoua, 01 P.O Box 801 Abidjan 01, Abidjan, Côte d'Ivoire.

DOI:

https://doi.org/10.30574/gscbps.2020.11.1.0049

Keywords:

Antispasmodic activity, Piliostigma reticulatum, Gastrointestinal disorders.

Abstract

Piliostigma reticulatum (Caesalpiniaceae) has been used in traditional medicine for the treatment of gastrointestinal disorders like diarrhea. The aim of the present study was to examine the relaxant activity of an extract of P. reticulatum stem bark and five of its fractions on isolated rabbit duodenum. Segments of duodenum of rabbits were suspended in an organ bath. The ethanol extract had been fractioned to obtain some fractions heptane, dichloromethane, ethyl acetate, butanol and aqueous extract. The ethanol extract and the dichloromethane fraction were tested on the contraction of rabbit duodenum at the increasing concentrations. Increasing concentrations of P. reticulatum ethanol extract and its fraction induced a relaxant effect on spontaneous rabbit duodenum. The ethanol extract reduced duodenum contraction totally at 1.32 mg/mL at IC₅₀ = 0.62 mg/mL. At the test concentration of 0.52mg/mL in organ bath, the dichloromethane fraction showed a relaxation of 100% (IC₅₀ = 0.24 mg/mL). Piliostigma reticulatum stem bark possesses antispasmodic properties that can at least explain and support its traditional use against diarrhea.

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