Prevalence of ESBL producing MDR E. coli and Klebsiellae pneumonae from clinical isolates of nosocomial hospital acquired infections

Nain Taara Bukhari; Aqsa Jameel; lashari Yasmeen; Hussain Atiya; M Ibrahim Urooj; Muneer Anum; Kazmi  Urooj

doi:10.30574/gscbps.2020.11.2.0131

Authors

Nain Taara Bukhari IIDRL lab Karachi University.
Aqsa Jameel IIDRL lab Karachi University.
lashari Yasmeen Dadabhoye institute of higher education.
Hussain Atiya Bolan university of medical and health sciences Quetta.
M Ibrahim Urooj IIDRL lab Karachi University.
Muneer Anum IIDRL lab Karachi University.
Kazmi Urooj IIDRL lab Karachi University.

DOI:

https://doi.org/10.30574/gscbps.2020.11.2.0131

Keywords:

MDR multidrug resistant, ESBL Extended spectrum of Beta Lactamase, NIs nosocomial infections, Pathogens, PCR polymerase chain reaction

Abstract

The impact of nosocomial infections (NIs) is an important public health concern due to emergence of new MDR strains of pathogens and approximately 5 to 10% of hospitalized patients are affected by NIs. 90,000 deaths per year in United States only warrant investigation. This study was a hospital based cross sectional study to determine the ESBL producing MDR nosocomial infection and their control from Jun 1st2016 to Dec31st 2017. 500 patients were investigated all demographic data was collected from patients with a history of hospital admission for ≥ 2 days. In this study out of total 500 patients, (62%) patients were female and (38%) were males, 19% isolates were E.coli and 18% were K.pneumonae all were MDR along with other pathogens .most of strains were showing 55%-85% resistance for more than one antibiotics. PCR studies show that SHV (32%) and TEM (18%) genes were found in ESBLs producing organisms. Due to importance of ESBL producing organisms and difficult treatment of infections caused by these bacteria rapid identification of ESBL producing strains should be adopted in enterobacteraciae, infection control strategies are recommended to adopt and warrant investigation.

Metrics

Metrics Loading ...

References

Allegranzi B, Nejad SB, Combescure C, Graafmans W, Attar H, Donaldson L and Pittet D. (2011). Burden of endemic health-care-associated infection in developing countries: systematic review and meta-analysis. The Lancet, 377(9761), 228-41.

Khan HA, Ahmad A and Mehboob R. (2015). Nosocomial infections and their control strategies. Asian pacific journal of tropical biomedicine, 5(7), 509-14.

Brusaferro S, Arnoldo L, Cattani G, Fabbro E, Cookson B, Gallagher R, Hartemann P, Holt J, Kalenic S, Popp W and Privitera G. (2015). Harmonizing and supporting infection control training in Europe. Journal of Hospital Infection, 89(4), 351-6.

Garner JS, Jarvis WR, Emori TG, Horan TC and Hughes JM. (1988). CDC definitions for nosocomial infections, 1988. American journal of infection control, 16(3), 128-40.

Custovic A, Smajlovic J, Hadzic S, Ahmetagic S, Tihic N and Hadzagic H. (2014). Epidemiological surveillance of bacterial nosocomial infections in the surgical intensive care unit. Materia socio-medica, 26(1), 7.

Llor C and Bjerrum L. (2014). Antimicrobial resistance: risk associated with antibiotic overuse and initiatives to reduce the problem. Therapeutic advances in drug safety, 5(6), 229-41.

Haas JP. (2006). Measurement of infection control department performance: state of the science. Am J Infect Control, 34(9), 543–9.

Mayhall GC. (2003). The epidemiology of burn wound infections: then and now. Clin Infect Dis, 37, 543–50.

Chandrasekar PH, Kruse JA and Mathews MF. (1986). Nosocomial infection among patients in different types of intensive care units at a city hospital. Critical care medicine, 14(5), 508-10.

Baqi S, Damani NN, Shah SA and Khanani R. (2009). Infection control at a government hospital in Pakistan. International Journal of Infection Control, 17, 5(1).

World Health Organization. (2014). Antimicrobial resistance: global report on surveillance. World Health Organization.

Wellington EM, Boxall AB, Cross P, Feil EJ, Gaze WH, Hawkey PM, Johnson-Rollings AS, Jones DL, Lee NM, Otten W and Thomas CM. (2013). The role of the natural environment in the emergence of antibiotic resistance in Gram-negative bacteria. The Lancet infectious diseases, 13(2), 155-65.

Zivanovic V, Gojkovic-Bukarica L, Scepanovic R, Vitorovic T, Novakovic R, Milanov N, Bukumiric Z, Carevic B, Trajkovic J, Rajkovic J and Djokic V. (2017). Differences in antimicrobial consumption, prescribing and isolation rate of multidrug resistant Klebsiella pneumoniae, Pseudomonas aeruginosa and Acinetobacter baumannii on surgical and medical wards. PloS one, 12(5), e0175689.

Solomon FB, Wadilo FW, Arota AA and Abraham YL. (2017). Antibiotic resistant airborne bacteria and their multidrug resistance pattern at University teaching referral Hospital in South Ethiopia. Annals of clinical microbiology and antimicrobials, 16(1), 29.

Sader HS, Farrell DJ, Flamm RK, et al. (2014). Antimicrobial susceptibility of Gram-negative organisms isolated from patients hospitalized with pneumonia in US and European hospitals: results from the SENTRY Antimicrobial Surveillance Program, 2009–2012. Int J Antimicrob Agents, 43, 328–334.

Beceiro A, Tomás M and Bou G. (2013). Antimicrobial resistance and virulence: a successful or deleterious association in the bacterial world? Clin Microbiol Rev, 26, 185–230.

Simor AE, Farley JE and Carroll KC. (2011). Genotypic and phenotypic characterization of methicillin-susceptible Staphylococcus aureus isolates misidentified as methicillin-resistant Staphylococcus aureus by the BD GeneOhm MRSA assay. J Clin Microbiol, 49, 1240– 1244.

Wintermans BB, Reuland EA, Wintermans RG, et al. (2013). The cost-effectiveness of ESBL detection: towards molecular detection methods? Clin Microbiol Infect, 19, 662–665.

Fujita S, Yosizaki K, Ogushi T, et al. (2011). Rapid identification of gram-negative bacteria with and without CTX-M extended-spectrum β-lactamase from positive blood culture bottles by PCR followed by microchip gel electrophoresis. J Clin Microbiol, 49, 1483–1488.