Functional and molecular characterization of plant growth promoting bacteria associated with sugarcane cultivated in Tay Ninh Province, Vietnam

Authors

  • Minh Tam Hoang Department of Natural Science Teacher Training, Sai Gon University, HCM City, Vietnam.
  • Thi Ngoc Thanh Dang Department of Natural Science Teacher Training, Sai Gon University, HCM City, Vietnam.
  • Ngoc Diệp Cao Dept. Microbiology Biotechnology, Biotechnology R&D Institute, Can Tho University, Can Tho City, Vietnam.

DOI:

https://doi.org/10.30574/gscbps.2020.11.2.0136

Keywords:

Plant associated bacteria, Plant growth promoting bacteria, Biological control, Nitrogen fixation, Sugarcane.

Abstract

Bacteria associated with sugarcane were investigated characteristics of plant growth promotion and identified base on molecular characteristics. Nitrogen fixation bacteria were isolated by using N-free Burk’s and LGI media. Pure colonies were then streaked on NBRIP medium to select phosphate solubilizing bacteria. The ability of producing IAA was screened by dropping Salkowski reagent into the bacterial suspension. There were 140 isolates of nitrogen-fixing and phosphate-solubilizing bacteria, and 41 isolates among them showed IAA producing. The abilities of producing IAA, fixing nitrogen, and solubilizing phosphate of these 41 isolates ranged from 1.9 to 12.8 mg L-1 IAA, 0.1 to 2.5 mg L-1 NH4+, and 32.4 - 286.5 mg L-1 P2O5, respectively. Thirteen selected isolates were preliminary tested biological control ability and identified base on 16S rDNA sequencing. There were 4 isolates capable of producing siderophore, 8 isolates inhibit Fusarium sp., 4 isolates inhibit Aspergillus niger, and one isolate capable of resisting both Escherichia coli and Staphylococcus aureus. The identification result showed these isolates belong to genera Stenotrophomonas, Bacillus, Chitinophaga, Burkholderia, Serratia, and Acinetobacter. These outstanding isolates were proposed to test the ability to promote sugarcane growth.

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References

Hartemink AE. (2008). Chapter 3 Sugarcane for Bioethanol: Soil and Environmental Issues, in Advances in Agronomy. Academic Press, 99, 125–182.

Duarah I, Deka M, Saikia N and Boruah HPD. (2011). Phosphate solubilizers enhance NPK fertilizer use efficiency in rice and legume cultivation. Biotech, 1(4), 227–238.

IPI Bulletin No. 21: Fertilizing for High Yield and Quality - Sugarcane.

Döbereiner J. (1992). Recent changes in concepts of plant bacteria interactions: endophytic N2 fixing bacteria. Ciência e Cultura, 44(5), 310-313.

Wood AW, Schroeder B and Dwyer R. (2010). Opportunities for improving the efficency of use of nitrogen fertiliser in the Australian sugar industry. 32nd Annual Conference of the Australian Society of Sugar Cane Technologist, 221–233.

Chapman LS, Halsall DM and Gibson AH. (1992). Biological nitrogenfixation and sugarcane. In Proceedings of Australian Society of Sugarcane Technologists, 14, 90–93.

Mehnaz S. (2011). Plant Growth-Promoting Bacteria Associated with Sugarcane. In: Maheshwari D. (eds) Bacteria in Agrobiology: Crop Ecosystems. Springer, Berlin, Heidelberg, 165–187.

Chaitanya K, Mahmood SK, Ranakausar and Sunil KN. (2014). Polymer producing bacteria showing siderophore activity with chrome azurol S (CAS) agar plate assay. International Journal of Scientific and Research Publications, 4 (12), 1-3.

Tay Ninh Statistics Office. (2017). Tay Ninh Province Statistical Yearbook 2017. Statistical Publishing House, Vietnam.

Trần T. (1999). Sugarcane planting techniques. Agriculture Publishing House, Vietnam.

Nguyen HH, Fani R, Le NT, Nguyen BN, Tran TNT and Pham TK Van. (2008). Isolation of endophytes for the production of biofertilizer at lab scale for the cultivation of sugarcane in Soc Trang province. Can Tho University Journal of Science, 8, 149–157.

Hoang MT and Cao ND. (2014). Characterization and identification of endophytic bacteria Gluconacetobacter diazotrophicus in sugarcane cultivated in Ben Tre and Long An provinces. Journal of Agriculture and Rural Development, 24, 68–74.

Hoang MT and Cao ND. (2014). Isolation, Characterization and Identification of Endophytic Bacteria in Sugarcane (Saccharum spp. L.) Cultivated on Soils of the Dong Nai Province, Southeast of Vietnam. American Journal of Life Sciences, 2(6), 361–368.

Hoang MT and Cao ND. Isolation and Identification of Rhizospheric Bacteria in Sugarcane (Saccharum spp. L.) Cultivated on Acrisols and Ferrasols of Dong Nai Province, the Southeast of Vietnam. American Journal of Life Sciences, 3(2), 109–118.

Nguyên HT, Hoang MT and Cao ND. (2014). Characterization of endophytic bacteria in sugarcane cultivated in Ben Tre and Long An provinces. Journal of Agriculture and Rural Development, 2, 41–48.

Cao ND and Bui TKO. Effect of Pseudomonas spp. on sugarcane yield and sugar concentration in sugarcane (Saccharum officinarum L.)(cv. VĐNL-7) cultivated on acid sulphate soil of Ben Luc district, Long An province. Can Tho University Journal of Science, 6, 69–76.

Cao ND, Nguyen TT, Vo VPQ and Nguyên VA. (2011). Effects of nitrogen-fixing Gluconacetobacter diazotrophicus and phosphatesolubilizing Pseudomonas stutzeri on sugarcane (Saccharum officinalis L.) cultivated on acid sulphate soil of Long An province. Can Tho University Journal of Science, 18b, 29–35.

Suman A, Yadav AN and Verma P. (2016). Endophytic Microbes in Crops: Diversity and Beneficial Impact for Sustainable Agriculture in Microbial Inoculants in Sustainable Agricultural Productivity. Springer India, 1, Research Perspectives, 117–143.

Luster J and Finlay R. (2006). Handbook of Methods Used in Rhizosphere Research. Swiss Federal Research Institute WSL, Birmensdorf, Switzerland.

Parka M, Kim CW, Yang C, Lee H, Shin W, Kim S and Sa T. (2004). Isolation and characterization of diazotrophic growth promoting bacteria from rhizosphere of agricultural crops of Korea. Microbiol. Res, 160(2), 127–133.

Nautiyal CS. (1999). An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS Microbiol. Lett, 170(1), 265–270.

Garg N, Garg KL and Mukerji KG. (2010). Laboratory Manual of Food Microbiology. I. K. International Pvt Ltd, India.

Graevenitz AV and Buche C. (1983). Accuracy of the KOH and vancomycin tests in determining the Gram reaction of non-enterobacterial rods. J Clin Microbiol, 18(4), 983–985.

Gordon SA and Weber RP. (1951). Colorımetrıc estımatıon of ındoleacetıc acıd. Plant Physiol, 26(1), 192–195.

Ventorino V. (2016). Lignocellulose-Adapted Endo-Cellulase Producing Streptomyces Strains for Bioconversion of Cellulose-Based Materials. Front Microbiol, 7, 1-15.

Solarzano L. (1969). Determination of ammonia in natural waters by the phenolhypochlorite methods. Limnology and Oceanography, 14(5), 799–801.

Murphy J and Riley JP. (1962). A modified single solution method for the determination of phosphate in natural waters. Analytica Chimica Acta, 27, 31–36.

Schwyn B and Neilands JB. Universal chemical assay for the detection and determination of siderophores. Anal. Biochem, 160 (1), 47–56.

Mew TW and Misra JK. (1994). A Manual of Rice Seed Health Testing. Int. Rice Res. Inst., Philippines.

Arora NK and Verma M. (2017). Modified microplate method for rapid and efficient estimation of siderophore produced by bacteria. Biotech, 7(6), 1-9.

Milagres AM, Machuca A and Napoleão D. (1999). Detection of siderophore production from several fungi and bacteria by a modification of chrome azurol S (CAS) agar plate assay. J. Microbiol. Methods, 7(1), 1–6.

Chaitanya K, Mahmood SK, Ranakausar and Sunilkumar NS. (2014). Polymer producing bacteria showing siderophore activity with chrome azurol S ( CAS ) agar plate assay. International Journal of Scientific and Research Publications, 4(12), 1-3.

Balouiri M, Sadiki M and Ibnsouda SK. (2016). Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis, 6(2), 71–79.

Hastuti RD, Lestari Y, Suwanto A and Saraswati R. (2012). Endophytic Streptomyces spp. as Biocontrol Agents of Rice Bacterial Leaf Blight Pathogen (Xanthomonas oryzae pv. oryzae). HAYATI Journal of Biosciences, 19 (4),155–162.

Shrivastava G, Kuma K and Yandigeri M. (2017). In vitro biocontrol activity of halotolerant Streptomyces aureofaciens K20: A potent antagonist against Macrophomina phaseolina (Tassi). Saudi Journal of Biological Sciences, 24, 192–199.

Kumar S, Stecher G, Li M, Knyaz C and Tamura K. “MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Mol. Biol. Evol, 35 (6), 1547–1549.

Baldani JI, Reis VM, Videira SS, Boddey LH and Baldani VLD. (2014). The art of isolating nitrogen-fixing bacteria from non-leguminous plants using N-free semi-solid media: a practical guide for microbiologists. Plant Soil, 384 (1), 413–431.

Dang TNT, Nguyen TXM and Cao ND. (2016). Indole acetic acid and siderophore production by selected isolates of plant associated bacteria and their effects on growth of maize (Zea mays L.) in pot experiments. Can Tho University Journal of Science, 47, 59–67.

Hoang MT, Dang TNT and Thai LNBT. (2019). Isolation and Characterization of Actinobacteria in Rhizosphere of Crinum latifolium (L.) Cultivated in Tay Ninh Province, Vietnam. International Journal of Innovations in Engineering and Technology, 13(4), 026-033.

Dang TNT and Do TT. (2018). Isolation and Characterization of Plant Growth Promoting Rhizobacteria in Black Pepper ( Piper nigrum L . ) Cultivated in Chon Thanh and LocNinh Districts of BinhPhuoc Province , Vietnam. International Journal of Innovations in Engineering and Technology (IJIET), 10(1), 01-010.

Nielsen A, Mansson M, Wietz M, Varming NA, Phipps KR, Larsen TO, Gram L and Ingmer H. (2012). Nigribactin, a novel siderophore from Vibrio nigripulchritudo, modulates Staphylococcus aureus virulence gene expression. Mar Drugs, 10(11), 2584–2595.

Xu S, Wang J, Wang H, Bao Y, Li Y, Govindaraju M, Yao W, Chen B and Zhang M. (2019). Molecular characterization of carbendazim resistance of Fusarium species complex that causes sugarcane pokkah boeng disease. BMC Genomics, 20(1), 115.

Girish K and Sushma G. (2018). Screening of bacteria for antifungal activity against phytopathogenic fungi of sugarcane (Saccharum officinarum L.). EurAsian Journal of BioSciences, 12, 195–203.

Sivasakthi S, Usharani G and Saranraj P. (2014). Biocontrol potentiality of plant growth promoting bacteria (PGPR) - Pseudomonas fluorescens and Bacillus subtilis: A review. Afr. J. Agric. Res., 9(16), 1265–1277.

Antwerpen TV, Rutherford R and Vogel J. (2001). Assessment of sugarcane endophytic bacteria and rhizospheric Burkholderia species as antifungal agents. Proc Annu Congr S Afr Sugar Technol Assoc, 76, 301–304.

Rooney AP, Dunlap CA and Flor-Weiler LB. (2016). Acinetobacter lactucae sp. nov., isolated from iceberg lettuce (Asteraceae: Lactuca sativa). Int. J. Syst. Evol. Microbiol, 66(9), 3566–3572.

Zhang CW, Zhang J, Zhao JJ, Zhao X, Zhao DF, Yin HQ and Zhang XX. (2017). Serratia oryzae sp. nov., isolated from rice stems. Int. J. Syst. Evol. Microbiol, 67(8), 2928–2933.

Radhakrishnan R, Hashem A and Allah EFA. (2017). Bacillus: A Biological Tool for Crop Improvement through Bio-Molecular Changes in Adverse Environments. Front Physiol, 8, 667.

Borriss R. (2011). Use of Plant-Associated Bacillus Strains as Biofertilizers and Biocontrol Agents in Agriculture. Springer, in Bacteria in Agrobiology: Plant Growth Responses, 41–76.

Hashem A, Tabassum B and Allah EFA. (2019). Bacillus subtilis: A plant-growth promoting rhizobacterium that also impacts biotic stress. Saudi Journal of Biological Sciences, 26 (6), 1291–1297.

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Published

2020-05-30

How to Cite

Hoang , M. T., Dang , T. N. T., & Cao , N. D. . (2020). Functional and molecular characterization of plant growth promoting bacteria associated with sugarcane cultivated in Tay Ninh Province, Vietnam. GSC Biological and Pharmaceutical Sciences, 11(2), 265–277. https://doi.org/10.30574/gscbps.2020.11.2.0136

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