Growth and survival promotion of a probiotic bacterium Enterococcus durans enriched Artemia nauplii on the prawn Macrobrachium rosenbergii

Indira Bhaheerathan Jain; Periyakali Saravana Bhavan; Chinnasamy Dharani; Thangaraj Manjula; Ramasamy Kalpana; Thirunavukkarasu Muralisankar; Madhayan Karthik; Paramasivam Puvitha

doi:10.30574/gscbps.2020.12.1.0201

Authors

Indira Bhaheerathan Jain Department of Zoology, Bharathiar University, Coimbatore – 641046, Tamil Nadu, India
Periyakali Saravana Bhavan Department of Zoology, Bharathiar University, Coimbatore – 641046, Tamil Nadu, India
Chinnasamy Dharani Department of Zoology, Bharathiar University, Coimbatore – 641046, Tamil Nadu, India
Thangaraj Manjula Department of Zoology, Bharathiar University, Coimbatore – 641046, Tamil Nadu, India
Ramasamy Kalpana Department of Zoology, Bharathiar University, Coimbatore – 641046, Tamil Nadu, India
Thirunavukkarasu Muralisankar Department of Zoology, Bharathiar University, Coimbatore – 641046, Tamil Nadu, India
Madhayan Karthik Department of Zoology, Bharathiar University, Coimbatore – 641046, Tamil Nadu, India
Paramasivam Puvitha Department of Zoology, Bharathiar University, Coimbatore – 641046, Tamil Nadu, India

DOI:

https://doi.org/10.30574/gscbps.2020.12.1.0201

Keywords:

Live feed, Artemia, Prawn, Probiotics, E. durans, Growth, Survival, Gut microflora, Digestive enzymes, Protein

Abstract

This work accentuate the growth performance of Macrobrachium rosenbergii post larvae (PL) fed with a probiotic bacterium, Enterococcus durans enriched live feed Artemia franciscana nauplii at five different serially diluted concentrations (10^-1, 10^-3, 10^-5, 10^-7 and 10^-9). After 45 days of the feeding trial, the survival rate, growth rate, activities of protease, amylase and lipase, and contents of total protein, amino acid, carbohydrate and lipid were significantly (P<0.05) increased, particularly at 1071×10^-7CFU. The gut microflora of un-enriched Artemia nauplii fed prawns showed the presence of Escherichia coli, Klebsiella sp., Citrobacter sp., Acinetobacter sp., Streptococcus sp., Bacillus sp., Staphylococcus sp., and Pseduomonas sp. The optimized concentration of E. durans enriched Artemia nauplii fed prawns showed the presence of Enterococcus sp., E. coli, Bacillus sp., and Klebsiella sp. The pathogenic bacteria, Citrobacter sp., Acinetobacter sp., Streptococcus sp., Staphylococcus sp., and Pseduomonas sp., were competitively excluded in the gut of prawns due to the colony establishment of Enterococcus sp., Hence, E. durans can be used as a enrichment material of Artemia nauplii for sustainable culture of M. rosenbergii.

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