Synthesis and evaluation of newly1-substituted-(2H)-2-thio-4-(3-substitutedthiocarbamido-1-yl)-6-(2-imino-4-thio-5- substitutedbiureto-1-yl) 1, 2-dihydro-S-triazines as potent antimicrobial agents

Abstract

The search for novel antibiotics is of immense importance in research areas around the world for agricultural, pharmaceutical, and industrial applications. The s-triazines are widely used as an important biological tool for the production of a wide range of novel secondary metabolites. In the present study, the series of 1-substituted-(2H)-2-thio-4-(3- substitutedthiocarbamido-1-yl)-6-(2-imino-4-thio- 5- substitutedbiureto-1-yl) 1,2-dihydro-s-triazine [4a(i) to 4f(iii)]  have been obtained by the isomerisation of 2-(2-imino-4-thio-5- substitutedbiureto-1-yl)-4-(3- substitutedthio-carbamido-1-yl)-6- substitutedimino-1,3,5-thiadiazine [3a(i) to 3f(iii)] in presence of ethanolic sodium bicarbonate solution. The later were synthesized by the chemical action of 1, 3-bis-(N-phenylamidinothiocarbamido)-thiocarbamide and aryl/alkylisocyanodichlorides. On the basis of IR, PMR spectrum data and elemental analysis, the structures of all these compounds were established. The synthesized s-triazine were analyzed for antimicrobial activities by cup plate diffusion method and exhibited a broad spectrum of antimicrobial activity against test pathogens. The isolate was tested for the ability to grow in the presence of antibiotic, such as ciprofloxacin at the same concentration. Thus, the study concludes with the eco-friendly route for synthesizing s-triazine with antibacterial activity against clinically important pathogens.