Assessment of lipid peroxidation, antioxidant enzyme superoxide dismutase, glutathione and serum homocysteine level in patients with polycystic ovary syndrome in Sudan Khartoum state

Ahmed Nassir Mohamed; Noon Babiker Mohammed; Abdelgadir Elamin Eltom; Amin Omer Abbas

doi:10.30574/gscbps.2020.12.3.0300

Authors

Ahmed Nassir Mohamed Department of Clinical Chemistry, Alfalah Medical Polyclinic,Taif, Saudi Arabia.
Noon Babiker Mohammed Associate professor in clinical chemistry University of Science and Technology, Sudan.
Abdelgadir Elamin Eltom Medical Laboratories Sciences, College of Health Sciences, Gulf Medical University, Ajman, UAE.
Amin Omer Abbas Department of Immunohematology, Albakkari Polyclinic, Almadinah Almunawarah, Saudi Arabia.

DOI:

https://doi.org/10.30574/gscbps.2020.12.3.0300

Keywords:

Polycystic ovary syndrome (PCOS), Lipid peroxidation, Antioxidant enzyme Superoxide dismutase

Abstract

Polycystic ovary syndrome (PCOS) is a common health problem that affects teenage girls and young women. PCOS is associated with a wide spectrum of complications in various parts of health, including reproductive, metabolic and psychological features. Analytical case control study was conducted to Assessment of Lipid peroxidation, antioxidant enzyme Super oxide dismutase, Glutathione and Serum Homocysteine level in Patients with (PCOS) in Khartoum State. A total of three hundred individuals were enrolled in this study, and classified into two groups, two hundred patients with (PCOS) as test group and hundred healthy individuals as control group, the samples were used to measure Homocysteine, Glutathione, Lipid peroxidation and superoxide dismutase level in the serum of participant with increased body mass index in study group and normal body mass index. The results were expressed as (mean ± SD).The mean ± standard deviation of the total glutathione was found mean 1174.5±271.5 and the reduced glutathione was (801.30 ±132.2), and for superoxide dismutase mean ± standard deviation (225.2, ± 57.8) in study group while in control group found 195.5, ± 25.6 respectively, Homocysteine mean ± standard deviation (14.9, ± 2.1) for test group and (12.1, ± 2.5) for control group. Lipid peroxidation mean ± standard deviation was found 3.4± 1.1 for test group and control group (2.4 ± 0.7) respectively. The present study has conculcated that mean of serum homocysteine, glutathione, lipid peroxide and superoxidase concentrations were increased in women with (PCOS), could be a dilemma for many women of reproductive ages irrespective of their reproductive disorders.

Metrics

Metrics Loading ...

References

Leila A, Najmeh T, Mansoureh M, Fahimeh RT, Saeedeh Z. Antioxidants and management of polycystic ovary syndrome in Iran: A systematic review of clinical trials. Iran J Reprod Med. 2015; 13(1): 1-8.

Norman RJ, Dewailly D, Legro RS, Hickey TE. Polycystic ovary syndrome. Lancet. 2007; 370(9588): 685-697.

Franks S, Berga S. Does PCOS have developmental origins? FertilSteril. 2012; 97: 2-6.

Abbott DH, Barnett DK, Bruns CM, Dumesic DA. Androgen excess fetal programming of female reproduction: A developmental aetiology for polycystic ovary syndrome? Hum Reprod Update. 2005; 11: 357-374.

Joham AE, Teede HJ, Ranasinha S, Zoungas S, Boyle J. Prevalence of infertility and use of fertility treatment in women with polycystic ovary syndrome: Data from a large community-based cohort study. J Wom Health. 2015; 24(4): 299-307.

Aubuchon M, Legro RS. Polycystic ovary syndrome: Current infertility management. ClinObstetGynecol. 2011; 54(4): 675–684.

American Congress of Obstetricians and Gynecologists. ACOG Practice Bulletin No. 108: Polycystic Ovary Syndrome. ObstetGynecol. 2009; 114(4): 936–949.

National Institutes of Health, Department of Health and Human Services. Beyond Infertility: Polycystic Ovary Syndrome (PCOS). NIH Pub. No. 08-5863, April 2008.

McFarland C. Treating polycystic ovary syndrome and infertility MCN Am J Matern Child Nurs. 2012; 37(2): 116–121.

Dewailly D, Lujan ME, Carmina E, Cedars MI, Laven J, Norman RJ, EscobarMorreale HF. Definition and significance of polycystic ovarianmorphology: a task force from the Androgen Excess and PolycysticOvary Syndrome Society. Human Reproduction Update. 2014; 20334–352.

Jenkins C, Wilson R, Roberts J, Miller H, McKillop J, Walker J. Antioxidants: Their Role in Pregnancy and Miscarriage. AntioxidRedox Signal. 2000; 2: 623-7.

Szczepanska M, Kozlik J, Skrzypczak J, Mikolajczyk M. Oxidativestress may be a piece in the endometriosis puzzle. FertilSteril. 2003; 79: 1288-93.

Gupta S, Agarwal A, Krajcir N, Alvarez JG. Role of oxidativestress in endometriosis. Reprod Biomed Online. 2006; 13(1): 12634.

Smith GJ, Ohl VS, Litwack G. Ligandin. The Glutathione S – Transferases, and chemically induced hepato carcinogenesis, A review, Cancer Research. 1977; 37: 8-14.

Loverro G, I orussoF, Mei L, Depalo R, Cormio G, Selvaggi L. The plasma homocysteine levels are increased in polycystic ovary syndrome. Gynecolobstet Invest. 2002; 53: 157-62.

Ahmed Badawy, Omnia State, Soma ShAbdElGawad and Omar Abd El Aziz. Plasmahomocysteine and polycystic ovary syndrome: themissed link. European Journal of Obstetrics and Gynaecology and Reproductive Biology. 2007; 131(1): 68– 72.

Mancini F, Cianciosi A, Reggiani GM, Facchinetti F, Battaglia C, de Aloysio D. Endothelial function and its relationship to leptin, homocysteine, and insulin resistance in lean and overweight eumenorrheic women and PCOS patients: a pilot study. FertilSteril. 2009; 91(6): 2537-44.

Lhan Tarkun, Berrin Çetinarslan, Zeynep Cantürk, Erdem Türemen. The Plasma Homocysteine Concentrations and Relationship with Insulin Resistance in Young Women with Polycystic Ovary Syndrome. Turkish Journal of Endocrinology and Metabolism. 2005; 1: 23-28.

Mohan SK, Priya VV. Lipid peroxidation, glutathione, ascorbic acid, vitamin E, antioxidant enzyme and serum homocysteine status in patients with polycystic ovary syndrome Biology and Medicine. 2009; 1(3): 44-49.

SagharSalehpour, Ozra Manzor-al-ajdad, Elham Neisani Samani, Alireza Abadi. Evaluation of Homocysteine Levels in Patients with Polycystic Ovarian Syndrome. Roya Institute. International Journal of Fertility and Sterility. 2011; 4(4): 168-71.

Schachter M, Raziel A, Friedler S, Strassburger D, Bern O, Ron-El R. Insulin resistance in patients with polycystic ovary syndrome is associated with elevated plasma homocysteine. Hum Reprod. 2003; 18: 721–27.

ALTOUM AEA, OSMAN AL, BABKER AM. Comparative study of levels of selective oxidative stress markers (malondialdehyde, zinc, and antioxidant vitamins A, E, and C) in ischemic and non-ischemic heart disease patients suffering from type-2 diabetes. Asian J Pharm Clin Res. 2018; 11(8): 508-510.

Ahmed Badawy, Omnia State, Soma Sh.Abd El Gawad, Omar Abd El Aziz. Plasma homocysteine and polycystic ovary syndrome: the missed link, European Journal of Obstetrics and Gynaecology and Reproductive Biology. 2007; 131(1): 68 – 72.