Simultaneous quantitative estimation of ellagic acid and gallic acid in Sudanese Solanum dubium seed by high performance thin layer chromatography (HPTLC)

Iman Tagelsir Abdalla Mohamed; Wasim Khan; Karishma Chester; Abdelwahab Hassan Mohamed; Sayeed Ahmad; Saad Mohamed Hussien Ayoub

doi:10.30574/gscbps.2020.13.1.0311

Authors

Iman Tagelsir Abdalla Mohamed Department of Chemistry, Preparatory College, University of Medical Sciences and Technology, Khartoum Sudan.
Wasim Khan Natural Product Laboratory, Faculty of Pharmacy, Hamdard University, Hamdard Nagar, New Delhi 110062,
Karishma Chester Natural Product Laboratory, Faculty of Pharmacy, Hamdard University, Hamdard Nagar, New Delhi 110062,
Abdelwahab Hassan Mohamed Department of Pharmacology, Faculty of Pharmacy, National Ribat, University, P.O. Box 2404 Khartoum Sudan.
Sayeed Ahmad Natural Product Laboratory, Faculty of Pharmacy, Hamdard University, Hamdard Nagar, New Delhi 110062,
Saad Mohamed Hussien Ayoub Department of Pharmacognosy, Faculty of Pharmacy, University of Medical Sciences and Technology, P.O. Box 12810, Khartoum, Sudan.

DOI:

https://doi.org/10.30574/gscbps.2020.13.1.0311

Keywords:

Sudanese Solanum dubium seed, Gallic acid, Ellagic acid, HPTLC

Abstract

Background: Solanum dubium is a plant believed to have several therapeutic effects including anti-asthmatic properties. The objective of this study was to investigate the quantitative estimation of Gallic acid and Ellagic acid from the seed extract of Sudanese Solanum dubium.

Methods: A simple and rapid high-performance thin-layer chromatographic method was developed and validated for quantitative estimation of Gallic acid and Ellagic acid from the seed extract of Sudanese Solanum dubium.

Results: Ellagic acid and Gallic acid were quantified by using HPTLC. The seeds were found to contain 1.1% w/w of Ellagic acid and 2.1% w/w of Gallic acid in extract. Gallic acid and Ellagic acid were chromatographed on silica gel 60 F254 TLC plate using Toluene: Ethyl acetate – Methanol – Formic acid (3:3:1:0.4 v/v/v/v) as mobile phase and quantified by densitometric scanning at 280 nm. The method was found to give compact spots for Gallic acid (Rf 0.35)

and Ellagic acid (Rf 0.21). The linear regression analysis data for standard Gallic and Ellagic acids spots showed good linear relationship with r2 = 0.988 and 0.994 respectively, in the concentration range 100-3000 ng/spot, accurate (99.23-102.7%), (98.7-100.2%); precise (% RSD ≤ 2); robust (% RSD ≤ 2) and specific. The LOD and LOQ of the method were found as 653 and 396.8ng/spot and 215.5and 130 ng/spot, of Gallic acid and Ellagic acid respectively.

Conclusion: The method was validated for precision, recovery and repeatability as per the International Conference on Harmonization Guidelines for Gallic acid and Ellagic acid. Statistical analysis of the data showed that the method is precise, accurate, reproducible and selective for the analysis of Gallic acid and Ellagic acid.

Metrics

Metrics Loading ...

References

Atkinson CJ, Nestby R, Ford YY, Dodds PAA. Enhancing beneficial antioxidants in fruits: A plant physiological perspective. BioFactors. 2005; 23:229–234.

Branch SK. Guidelines from the International Conference on Harmonisation (ICH). Journal of Pharmaceutical and Biomedical Analysis. 2005; 38[5]:798- 805.

Clifford MN, Scalbert A. Ellagitannins – native, occurrence and dietary burden. Journal of the Science of Food and Agriculture. 2000; 80: 1118– 1125.

Gupta Mradu, Sasmal Saumyakanti, Majumdar Sohini and Mukherjee Arup, HPLC Profiles of Standard Phenolic Compounds Present in Medicinal Plants. International Journal of Pharmacognosy and Phytochemical Research. 2012; 4 [3]: 162- 167.

Kamal YT, Mohammed MS, Singh M, Parveen R, Ahmad S, Baboota S, Ali I, Siddiqui KM,Arif ZSM. Development and validation of HPLC method for simultaneous estimation of piperine and guggulsterones in compound Unani formulation (tablets) and a nanoreservoir system, Biomedical Chromatography. 2012; 26: 1183-1190.

Ravishankara MN,ShrivastavaN, Jayathirtha MG, Padh H, Rajani M. A sensitive High-performance thin layer chromatographic method for the estimation of diospyrin, a tumour inhibitory agent from the stem bark of Diospyrosmontana Roxb. J. Chromatography B. 2000; 744: 257-262.

Rebecca JR. Phenolic acids in foods: An overview of analytical methodology. J. Agric. Food Chem. 2003; 51[10]: 2866-2887.

Salih HM. Investigation of alkaloidal content of certain Solanum species. M.Sc. Thesis, University of Khartoum, Sudan. 1979.

Sheng Teng Huang, Chen Yu Wang, Rong Chi Yang, Hsiao-Ting Wu, Su-Hui Yang, Yung-Chi Cheng, Jong-Hwei S Pang. Ellagic Acid, the Active Compound of Phyllanthus urinaria, Exerts In Vivo Anti- Angiogenic Effect and Inhibits MMP-2 Activity. Evidence-Based Complementary and Alternative Medicine. 2011; 1-10.

Singh MYT, Kamal R Parveen, Ahmad S. Development and validation of a stability-indicating HPTLC method for analysis of arjunolic acid in a herbal formulation, J planar Chromatography. 2011; 24: 172-175.

Weerasak Samee, Suwanna Vorarat, Simultaneous determination of gallic acid, catechin, rutin, ellagic acid and quercetin in flower extracts of Michelia Alba, Caesalpinia pulcherrima and Nelumbo nucifera by HPLC. Thai Pharmaceutical and Health Science Journal. 2007; 2 [2]:131-137.