Dietary supplementation with manganese and selenium offer protection against cadmium-induced oxidative damage to rat liver and kidney

Simon Gabriel Mafulul; Enoch Banbilbwa Joel; Chukwudi Acha Orji; Comfort Sokomba Edah; Larry Auta Barde; Francis Obiora Okonkwo

doi:10.30574/gscbps.2020.13.1.0339

Authors

Simon Gabriel Mafulul Department of Biochemistry, Faculty of Basic Medical Sciences, University of Jos, Jos, Plateau State, Nigeria.
Enoch Banbilbwa Joel Department of Biochemistry, Faculty of Basic Medical Sciences, University of Jos, Jos, Plateau State, Nigeria.
Chukwudi Acha Orji Department of Biochemistry, Faculty of Basic Medical Sciences, University of Jos, Jos, Plateau State, Nigeria.
Comfort Sokomba Edah Department of Biochemistry, Faculty of Basic Medical Sciences, University of Jos, Jos, Plateau State, Nigeria.
Larry Auta Barde Department of Biochemistry, Faculty of Natural and Applied Sciences, Plateau State University, Bokkos, Plateau State, Nigeria.
Francis Obiora Okonkwo Department of Biochemistry, Faculty of Natural and Applied Sciences, Plateau State University, Bokkos, Plateau State, Nigeria.

DOI:

https://doi.org/10.30574/gscbps.2020.13.1.0339

Keywords:

Cadmium, Selenium, Manganese, Pre-supplementation, Antioxidants, lipid peroxidation

Abstract

The present study determined the effect of pre-supplementation with manganese (Mn) and selenium (Se) on biomarkers of oxidative stress in the liver and kidneys of rats exposed to a mild dose of cadmium. Sixteen Male Wistar strain rats (180-200 g b. wt) were divided into four groups (control, Cd alone, Mn + Se + Cd and Mn + Se). The rats used as the control received a normal rat diet and tap water throughout the study while the Cd alone rats received a normal rat diet and then exposed to a single daily oral dose of cadmium (3 mg CdCl2/kg) in drinking water for three days. Mn + Se + Cd rats were pretreated with Mn (3 mg MnCl₂/kg/day) and Se (3mg SeO₂/kg/day) for seven days and thereafter received a single daily oral dose of cadmium (3 mg CdCl₂/kg) in drinking water for three days while Mn + Se rats were exposed to only Mn (3 mg MnCl₂/kg/day) and Se (3mg SeO₂/kg/day) for seven days. At the end of the experiment tissue cadmium concentration, membrane lipid peroxidation, glutathione content, and activities of antioxidant enzymes catalase, superoxide dismutase, and glutathione peroxidase were determined in the liver and kidney samples. The results showed that pretreatment with Mn and Se effectively countered Cd-induced cadmium accumulation, membrane lipid peroxidation, depletion of the non-enzymic antioxidant, glutathione, and induction of the antioxidant enzymes catalase, superoxide dismutase and glutathione peroxidase in the liver and kidney. It can be concluded that pre-supplementation with Mn and Se significantly reversed Cd-induced deleterious alterations in the liver and kidney tissue of the rats.

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