Determination of polyphenolic components by high performance liquid chromatography (HPLC) and evaluation of the antioxidant activity of leaves and fruits of Crataegus mongyna Jacq

Nihed Ziouche; Leila Derradji; Youcef Hadef

doi:10.30574/gscbps.2020.13.1.0344

Authors

Nihed Ziouche Laboratory of Pharmacognosy, Department of Pharmacy, Faculty of Medicine, Badji Mokhtar-Annaba University, Algeria.
Leila Derradji Laboratory of Pharmacognosy, Department of Pharmacy, Faculty of Medicine, Badji Mokhtar-Annaba University, Algeria.
Youcef Hadef Laboratory of Development and Control of Pharmaceutical Preparations, Department of Pharmacy, Faculty of Medicine, Badji Mokhtar-Annaba University, Algeria.

DOI:

https://doi.org/10.30574/gscbps.2020.13.1.0344

Keywords:

Crataegus mongyna Jacq, Leaves, Fruits, Methanolic extracts, Polyphenolic components, Antioxidant activity

Abstract

Crataegus mongyna Jacq is an endemic species of eastern Algeria, used in traditional medicine for its many therapeutic virtues. Its leaves and fruits are rich in polyphenols. In the present study we wanted to research its polyphenolic components in which it is rich. The method we used is high performance liquid chromatography (HPLC) and evaluation of the antioxidant activity in the methanolic extracts of its leaves and fruits. For this evaluation we followed the free radical reduction method DPPH (2, 2-diphenyl-1-picryl hydrazyl) by an antioxidant. The results show, the presence of polyphenolic components such as rutin, quercetin and isoquercetin and significant antioxidant activity. Crataegus monogyna Jacq is could be placed as an alternative treatment for certain pathologies because it is of value for public health

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References

Barceloux DG. (2008). Medical toxicology of natural substances: Foods, fungi, medicinal herbs, plants, and venomous animals. John Wiley & Sons

Nagata T and Ebizuka Y. (2013). Medicinal and aromatic plants XII. Springer Science & Business Media; 348.

Laraba M, Serrat A and Ouassaa G. (2016). Étude in vitro de l’activité antioxydante des polyphénols isolés à partir d’une plante médicinale; 48-52

Ferrari J and Hostettmann K. (2002). Contribution à la connaissance du métabolisme secondaires des Thymelaceae et investigation phytochimique de l’une d’elle: « Gnidia involucrata » Steud, ex A. Rich. University of Lausanne ; 200

Prinz S, Ringl A, Huefner A, Pemp E and Kopp B. (2007). 4′′′‐Acetylvitexin‐2 ″‐O‐rhamnoside, Iso-orientin, Orientin, and 8‐Methoxykaempferol‐3‐O‐glucoside as Markers for the Differentiation of Crataegus monogyna and Crataegus pentagyna from Crataegus laevigata (Rosaceae). Chemistry & biodiversity; 2920-2931.

Sagaradze V, Babaeva E and Kalenikova, E. (2017). HPLC-UV method for determing flavonoids in Hawthorn flowers and leaves. Pharmaceutical Chemistry Journal; 277-280

Mraihi F, Journi M, Chérif J, Sokmen M, Sokmen A, and Trabelsi A M. (2013). Phenolic contents and antioxidant potential of Crataegus fruits grown in Tunisia as determined by DPPH, FRAP, and β-carotene/linoleic acid assay. Journal of chemistry. University of Carthage; 4-5

Barros L, Carvalho A and Ferreira I. (2011). Comparing the composition and bioactivity of Crataegus monogyna flowers and fruits used in folk medicine. Phytochemical analysis; 181-188.