Cardioprotective effect of the aqueous extract of seeds of Datura metel (Solanaceae) on acute cardiotoxicity induced with doxorubicin in Wistar rats

Mbida Hacheked; Tsala David Emery; Aboubakar Sidiki; Amang André Perfusion; Ze Minkande Jacqueline

doi:10.30574/gscbps.2020.13.3.0350

Authors

Mbida Hacheked Department of Biological Sciences, Faculty of Science, University of Maroua, P.O. Box 814, Maroua, Cameroon.
Tsala David Emery Institute of Agricultural Research for Development, P.O. Box 167, Meyomessala, Cameroon.
Aboubakar Sidiki Department of Biological Sciences, Faculty of Science, University of Maroua, P.O. Box 814, Maroua, Cameroon.
Amang André Perfusion Department of Biological Sciences, Faculty of Science, University of Maroua, P.O. Box 814, Maroua, Cameroon.
Ze Minkande Jacqueline Faculty of Medicine and Biomedical Sciences, University of Yaoundé I, P.O. Box 1364, Yaoundé, Cameroon.

DOI:

https://doi.org/10.30574/gscbps.2020.13.3.0350

Keywords:

Datura metel, Cardioprotection, Cardiotoxicity, Doxorubicin, Antioxydant

Abstract

The current study evaluated the cardioprotective effect of the aqueous extract of seeds of Datura metel (AESDM) on the acute cardiotoxicity induced with doxorubicin in the Wistar rats. Indeed, 30 rats have been randomized then, divided into 6 groups of 5 animals each: 3 control groups (normal, negative and positive) and 3 test groups (AESDM 100, 200 and 300 mg/kg). These animals received orally, six consecutive days, 1 mL/200 g distilled water (normal and negative control), 100 mg/kg Vitamin-E (positive control), AESDM at 100, 200 and 300 mg/kg (test groups). On the seventh day of treatment, animals in the different groups excepted the normal control received a single intraperitoneal injection of 15 mg/kg doxorubicin followed respective treatments up to the tenth day of the trial period. Serum parameters (ASAT/ALAT), biochemical markers of oxidative stress (MDA, SOD, CAT, GSH) and lipid parameters (total cholesterol, triglycerides, HDL and LDL) were evaluated. Histological sections of animal hearts were prepared. The results of this study showed that treatment with AESDM resulted in a significant decrease (P ˂ 0.001) in MDA, triglycerides and LDL-cholesterol levels, ASAT/ALAT activity and a significant increase (P ˂ 0.001) in HDL-cholesterol concentration and SOD, CAT and GSH activities, compared to the negative control. Histological observation revealed that AESDM protected the heart from doxorubicin-induced damage. In conclusion, AESDM would have a cardioprotective effect that could be attributed to its antioxidant potential.

Metrics

Metrics Loading ...

References

WHO. The top 10 causes of death. WHO Media Centre: the top 10 causes of death. 2017.

Boombhi JH, Menanga A, Nkoke C, Hamadou B, Kingue S. Variabilité sinusale chez un sujet en Insuffisance cardiaque congestive à Yaoundé. Health sciences and disease. 2016; 17(1): 1-5.

Agbor V, Essouma M, Ntusi N, Nyanga UF, Bigna JJR, Noubiap JJ. Heart failure in sub-Saharan Africa: A contemporaneous systematic review and meta-analysis. International Journal of Cardiology. 2018; 257: 207-215.

Damasceno A, Mayosi B, Sani M et al. The causes, treatment, and outcome of acute heart failure in 1006 Africans from 9 countries. Archive of International Medicine. 2012; 172: 1386-1394.

Clovis N, Ahmadou M, Leopold N, Denis GT, Nkoualack DC, Anastase D. Heart failure in semi-urban setting in Cameroun: clinical characteristics, etiologies, treatment and outcome. Journal of Xiangya Medecine. 2019; 4: 6-9.

Prabhu S, Mallika J, Sabitha KE, Shyamaladevi CS. Cardioprotective effect of magniferin on isoproterenol induced myocardial infraction in rats. Indian Journal of Experimental Biology. 2006; 44: 209-215.

Mercadier J. Approches physiopathologiques actuelles de l’insuffisance cardiaque. Presse Médicale. 2007; 36: 979-984.

McMurray J, Adamopoulos S, Anker S et al. Society of Cardiology guidelines for the diagnosis and treatment of acute and chronic heart failure. European Journal of Heart Failure. 2012; 14: 803-869.

Yusuf S, Hawken S, Ounpuu TS et al. Effect of potential unmodifiable risk factors associated with myocardial infarction in 52 countries (the inter heart study): case-control study. Lancet. 2004; 364: 937-952.

Takano H, Zou Y, Hasegawa H et al. Oxidative stress-induced signal transduction pathways in cardiac myocytes: involvement of ROS in heart diseases. Antioxidant and Redox Signaling. 2003; 5: 789-94.

Monteil C, Mulder P, Thuillez C. oxydant et insuffisance cardiaque: une cible thérapeutique utopique? Médecine Thérapeutique de Cardiologie. 2004; 2: 78-85.

Mukhopadhyay P, Rajesh M, Bátkai S, Kashiwaya Y, Haskó G, Liaudet L, Szabó C, Pacher P. Role of superoxide, nitric oxide, and peroxynitrite in doxorubicin-induced cell death in vivo and in vitro. American Journal of Physiology- Heart and Circulatory Physiology. 2009; 296(5): 1466-1483.

Sterba M, Popelová O, Vávrová A, Jirkovský E, Kovaríková P, Geršl V and Simunek T. Oxidative stress, redox signaling, and metal chelation in anthracycline cardiotoxicity and pharmacological cardioprotection. Antioxidant and Redox Signaling. 2013; 18(8): 899-929.

Yang F, Teves SS, Kemp CJ, Henikoff S. Doxorubicin, DNA torsion, and chromatin dynamics. Biochemistry and Biophysics Acta. 2014; 1845(1): 84-9.

Asselin BL, Meenakshi D, Chen L, Vivian IF, Jeanette P, Michael JB, Robert EH, Yaddanapudi R, Saro HA, Bruce MC, Steven EL. Cardioprotection and safety of dexrazoxane in patients treated for newly diagnosed T-Cell acute lymphoblastic leukemia or advanced-stage lymphoblastic non-hodgkin lymphoma. Journal of Clinical Oncology. 2016; 34(8): 854-862.

Bhupalam P, Akkiraju S, Talla S, Kanala SR, Gouruntla N, Kastury VH. Cardioprotective activity of flavonoid fraction of Gymnema sylvestre leaves on doxorubicin induced cardiac damage. Journal of Young Pharmacists. 2018; 10: 422-426.

Kurek-Górecka A, Rzepecka-Stojko A, Górecki M, Stojko J, Sosada M and Swierczek- Zieba G. Structure and antioxidant activity of polyphenols derived from propolis. Molecules. 2013; 19(1): 78-101.

Georgiev V, Ananga A, Tsolova V. Recent advances and uses of grape flavonoids as nutraceuticals. Nutrients. 2014; 6(1): 391-415.

Bayih T. Synergistic bioeficacy of insecticidal plants against bean bruchids (Zabrotes subfasciatus: Coleoptera) a major storage pests of common bean (Phaseolus vulgaris L.) in central rift valley of Ethiopia. MSc thesis, Department of Biology, School of Graduate Studies, Haramaya University. 2014.

Kayode A, Chinedu I, Chukwuma S, Zuhairah I. Effects of ethanolic extracts of Datura metel on blood lipid profile of male albino rats. International Journal of Scientific Reports. 2014; 2: 248-252.

Tsala DE, Mbida H, Nnanga N, Ngo LTE, Habtemariam S, Ze MJ. Mechanism and inotropic actions of the water extracts of the leaves and seeds of Datura metel (Solanaceae) on isolated frog’s heart. American Journal of Physiology, Biochemistry and Pharmacology. 2020; 10(2): 40-47.

Jakabová S, Vincze L, Farkas A et al. Determination of tropane alkaloids atropine and scopolamine by liquid chromatography-mass spectrometry in plant organs of Datura species. Journal of Chromatography. 2012; 1232: 295-301.

Al- Snaifi A. Medical importance of Datura fastuosa (syn: Datura metel) and Datura stramonium. International Organization of Scientific Research Journal of Pharmacy. 2017; 7: 43-58.

Du Y, Lou H. Catechin and proanthocyanidin B4 from grape seeds prevent doxorubicin-induced toxicity in cardiomyocytes. European Journal of Pharmacology. 2008; 591(3): 96-101.

Vincent DT, Ibrahim YF, Espey MG, Suzuki YJ. The role of antioxidants in the erea of cardio-oncology. Cancer Chemotherapy Pharmacology. 2013; 72(6): 1157-1168.

Harborne JB. Textbook of Phytochemical Methods. A Guide to Modern Techniques of Plant Analysis. 5 Edition. Chapman and Hall Ltd, London. 1998; 21-72.

Saeed NM, El-Naga RN, El-Bakly WM, Abdel-Rahman HM, Salah ElDin RA, El-Demerdash E. Epigallocatechin-3-gallate pretreatment attenuates doxorubicin-induced Cardiotoxicity in rats: A mechanistic study. Biochemistry and Pharmacology. 2015; 95(3): 145-55.

Murray R. Aspartate aminotransferase, Alanine aminotransferase, Creatinine. Clinical Chemistry. 1984; 1112-116.

Wilbur KM, Bernheim F, Shapiro OW. Determination of lipid peroxidation. Arch Biochemistry and Biophysics. 1949; 24: 305-310.

Misra HP, Fridovich I. The role of super oxide anion in the auto oxidation epinephrine to adrénochrome and a simple assay for superoxide dismutase. Journal of Biological Chemistry. 1972; 247: 3170-3175.

Sinha AK. Colorimetric assay of catalase. Anal of Biochemistry. 1972; 47: 389-394.

Ellman GL. Tissue sulfhydryl group. Archives of Biochemistry and Biophysics. 1959; 82: 70-77.

Parekh AC, Jung DH. Cholesterol determination with ferric acetate-uranium acetate and sulfuric acid-ferrous sulfate reagents. Analytical Chemistry. 1970; 42: 1423-1427.

Rice EW. Standard method of clinical chemistry. New York: Academic Press. 1970.

Burstein M, Scholnick HR. Lipoprotein-polyanionmetal interactions. Advances in Lipid Research. 1973; 11: 67-108.

Judson I, Verweij J, Gelderblom H, Hartmann JT, Schöffski P, Blay JY. Doxorubicin alone versus intensified doxorubicin plus ifosfamide for first-line treatment of advanced or metastatic soft-tissue sarcoma: a randomised controlled phase 3 trial. Lancet Oncology. 2014; 15(4): 415-423.

Mustafa HN, El-Awdan SA, Hegazy GA, Abdel JGA. Prophylactic role of coenzyme Q10 and Cynara scolymus L on doxorubicin-induced toxicity in rats: Biochemical and immunohistochemical study. Indian Journal of Pharmacology. 2005; 47(6): 649-56.

Chatterjee K, Zhang J, Hombo N and Karliner J. Doxorubicin Cardiomyopathy. Cardiology. 2010; 115(2): 155-162.

Delemasure S, Vergely C, Zeller M, Cottin Y, Rochette L. Prévention de la cardiotoxicité des anthracyclines : approche fondamentale des mécanismes mis en jeu ; relations avec les données cliniques. Annales de Cardiologie et d’Angéiologie. 2006; 55: 104-112.

Sterba M, Popelová O, Vávrová A, Jirkovský E, Kovaríková P, Geršl V and Simunek T. Oxidative stress, redox signaling, and metal chelation in anthracycline cardiotoxicity and pharmacological cardioprotection. Antioxidant and Redox Signaling. 2013; 18(8): 899-929.

Colombo A, Cipolla C, Beggiato M, Cardinale D. Cardiac toxicity of anticancer agents. Current Cardiology Reports. 2013; 15: 357-362.

Octavia Y, Tocchetti C, Gabrielson K et al. Doxorubicin-induced cardiomyopathy: from molecular mechanisms to therapeutic strategies. Journal of Molecular and Cellular Cardiology. 2012; 52: 1213-1225.

Akkiraju SYP, Venkatesh P, Ranjith KK, Suma V, Rojapathi NM. Cardioprotective Effect of Clove Oil in Isoprenaline Induced Myocardial Infarction on Male Wistar Rats: Ethnopharmacology. 2014; 2: 1-3.

Kamoun P, Lavoinne A, De Verneuil. Biochimie et biologie moléculaire. EDS Flammarion Médecine-sciences. 2003; 114-115.

Walker DB. Serum Chemical Biomarkers of Cardiac injury for Nonclinical Safety Testing. Toxicologic Pathology. 2006; 34: 94-104.

Mazevet M, Moulin M, Llach-Martinez A et al. Complications of chemotherapy, a basic science update. Presse Medicale. 2013; 42: 352-61.

Barnabé N, Zastre JA, Venkataram S, Hasinoff BB. Deferiprone protects against doxorubicin-induced myocyte cytotoxicity. Free radical biology and Medicine. 2002; 33 (2): 266-275.

Yagmurca M, Fadillioglu E, Erdogan H, Ucar M, Sogut S, Irmak MK. Erdosteine prevents doxorubicin-induced cardiotoxicity in rats. Pharmacology Research. 2003; 48(4): 377-382.

Ioanna A, Fragiska S, Efstathios KI, Maria P, Constantinos S, Nektarios A, Paraskevi S, Vassilis G, Efstathios P, Dimitrios TK. Acute doxorubicin cardiotoxicity is successfully treated with the phytochemical oleuropein through suppression of oxidative and nitrosative stress. Journal of Molecular and Cellular Cardiology. 2007; 42: 549-558.

El-Boghdady NA. Antioxidant and antiapoptotic effects of proanthocyanidin and Ginkgo biloba extract against doxorubicin-induced cardiac injury in rats. Cell Biochemistry Function. 2013; 31: 344-351.

Kara AW, Ihoual S, Abidli N. The Combination Therapy of Medicinal Plant Globularia alypum, with Adriamycin Limits Free Radical Mediated Cardiac Injury in Rats. International Journal of Pharmaceutical Sciences Review and Research. 2016; 36(1): 1-8.

Hassan M, El-Beshbishy H, Aly H et al. Modulatory effects of meloxicam on cardiotoxicity and antitumor activity of doxorubicin in mice. Cancer Chemotherapy and Pharmacology. 2014; 74: 559-569.

Zhang XJ, Cao XQ, Zhang CS, Zhao Z. 17ß-estradiol protects against doxorubicin-induced cardiotoxicity in male Sprague-Dawley rats by regulating NADPH oxidase and apoptosis genes. Molecular Medicine Reports 2017; 15(5): 2695-2702.

Iarussi D, Indolfi F, Casale P et al. Recent advances in the prevention of anthracycline cardiotoxicity in childhood. Current Medicinal Chemistry. 2001; 8: 1649-1660.

Xu X, Persson HL, Richardson DR. Molecular pharmacology of the interaction of anthracyclines with iron. Molecular Pharmacology. 2005; 68(2): 261-271.

Bandari U, Shweta S, Madhusudhana K, Bidya DS, Challa VS, Rajeswara RP, Naidu VGM, Ramakrishna S. Ethanolic extract of Boswellia ovalifoliolata bark and leaf attenuates doxorubicin-induced cardiotoxicity in mice. Environmental toxicology and pharmacology. 2013; 36: 840-849.

Abdalla A. Ameliorative influence of dietary dates on doxorubicin-induced cardiac toxicity. Intensive Care Medicine,. 2016; 7: 343-353.

Mazunder UK, Gupta M, Rajeshwar Y. Antihyperglycemic effect and antioxidant potential of Phyllanthus niruri (Euphorbiaceae) in streptozotocin induced diabetic rats. European Bulletin of Drug Research. 2005; 13(1): 13 - 23.

Favier A. conceptuel et expérimental dans la compréhension des mécanismes des maladies et potentiel thérapeutique. L’actualité Chimique. 2003; 108 - 115.

Milane H. La quercétine et ses dérivés: molécules à caractère prooxydant ou capteurs de radicaux libres; études et applications thérapeutiques. Thèse de doctorat de l’université de Louis Pasteur. 2004; 13-36.

Abba P, Souleymane M, Lydie B, Dodehe Y, Tanoh H, Jean D. Cardioprotective and anti-inflammatory activities of polyphenols enriched extract of Hibiscus sabdariffa petal extracts in wistar rats. Journal of Pharmacognosy and Phytochemistry. 2015; 4(1): 57-63.

Meaad FS, Fawzia AA, Othman ASB, Mazin AZ, Lobna S, Ibrahim AH, Aymn TA, Mohamed KA. Cardioprotective effect of Ajwa date aqueous extract on doxorubicine-induiced toxicity in rats. Biomedical and pharmacology Journal. 2018; 11(3): 1521-1536.

Sathishsekar D, Subramanian S. Antioxidant properties of Momordica charantia (Bitter gourd) seeds on Streptozotocin induced diabetic rats. Asia Pacific Journal of Clinical Nutrition. 2005; 14 (2): 153- 158.

Velavan S, Selvarani S, Adhithan A. Cardioprotective effect of Trichopus zeylanicus against myocardial ischemia induced by isoproterenol in rats. Bangladesh Journal of Pharmacology. 2009; 4: 88-91.

Gan J, Feng Y, He Z, Li X, Zhang H. Correlations between Antioxidant Activity and Alkaloids and Phenols of Maca (Lepidium meyenii), Journal of Food Quality. 2017; 1-10.

Anila L, Vijayalakshmi NR. Flavonoids from Emblica officinalis and Mangifera indica-effectiveness for dyslipidemia. Journal of Ethnopharmacology. 2002; 79(1): 81-87.

Abdelbaky N, Ali A, Raeesa M. Cardioprotective effect of simvastatin on doxorubicin induced oxidative cardiotoxicity in rats. Australian Journal of Basic and Applied Sciences. 2010; (1): 29-38.