Evaluation of the hemogram of Oryctolagus cuniculus envenomus

Obou Constantin Okou; N’guessan Emmanuel Assemian; Kouadio Bernard Allali; Guy Childeric Bingo; Allico Joseph Djaman

doi:10.30574/gscbps.2020.13.2.0384

Authors

Obou Constantin Okou Department of Biochemistry and Microbiology, Agroforestry Training and Research Unit, Jean Lorougnon Guédé University, Côte d'Ivoire.
N’guessan Emmanuel Assemian Department of Biology and Tropical Ecology, Environmental Training and Research Unit, Jean Lorougnon Guédé University, Côte d'Ivoire.
Kouadio Bernard Allali Environment and Health Department, Entomology and Herpetology Unit, Pasteur Institute of Côte d'Ivoire.
Guy Childeric Bingo Department of Biochemistry and Microbiology, Agroforestry Training and Research Unit, Jean Lorougnon Guédé University, Côte d'Ivoire.
Allico Joseph Djaman Department of Biology-Health, Biosciences Training and Research Unit, Félix Houphouët-Boigny University Abidjan, Côte d’Ivoire.

DOI:

https://doi.org/10.30574/gscbps.2020.13.2.0384

Keywords:

Venom, Naja nigricollis, Haemogram

Abstract

The overall objective of this study was to evaluate the hemolysing action of Naja nigricollis venom on rabbit blood. To carry out this study, three batches of three rabbits were formed with two control batches and one experimental batch. Each control lot is composed of three rabbits (males or females) while the experimental lot is composed of two males and one female. Each rabbit from the control lots was separately collected in the purple tube (EDTA) and transported to the laboratory for analysis. The rabbits from the experimental batch were also collected distinctly a few minutes after the injection of the venom of Naja nigricollis for the analysis of haematological parameters. However, before the analysis of the hematological parameters of the rabbits from the control and experimental batches, an in vitro hemolysis test of Naja nigricollis venom was performed to verify its hemolysing power. The results showed that Naja nigricollis venom has a dose-dependent in vitro hemolysing power. As for the haemogram, it revealed that the venom of Naja nigricollis has a decreasing effect on blood cells (red and white blood cells), on haemoglobin and on haematocrit, and an elevation on MGVs thus promoting anaemia.

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