Modification of atherogenic diet causes atherosclerosis, increase total cholesterol, and showing hepar damage in mice as alternative animal model in atherosclerosis research
DOI:
https://doi.org/10.30574/gscbps.2021.14.2.0402Keywords:
Modified atherogenic diet, Atherosclerosis, Foam cell, Total cholesterol, Mice, Hepar damageAbstract
This study aims to determine the composition of the ideal atherogenic diet to increase the risk of atherosclerosis in the animal model. The research was used 15 male mice, acclimatized for 1-2 weeks to get the susceptible amount of diet to be given. The animal objects are divided into 3 groups; normal diet group (control), 4 weeks atherogenic diet groups, and 8 weeks atherogenic diet groups. The modified atherogenic diet consists of common broiler food, wheat flour, pork oil, quail egg yolks, and water, given 2 times a day of 30 g of food. Water for animal objects is given ad libitum. After 4 weeks and 8 weeks total cholesterol, the formation of foam cells, and hepatocyte degeneration. The results show that the total blood cholesterol of animal models in the group of 8 weeks has the highest level (153, 66 ± 6, 51), compared to other groups (normal diet and 4 weeks). Animal models show that aortic cross-section formed foam cells in tunica intima and tunica media of endotel, also show the indication of hepar damage by hepatocyte degeneration.
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References
A. Eisen et al., “Angina and future cardiovascular events in stable patients with coronary artery disease: Insights from the Reduction of Atherothrombosis for Continued Health (REACH) registry,” J. Am. Heart Assoc. 2016; 5(10).
K Riches, KE Porter. Lipoprotein(a): Cellular effects and molecular mechanisms Cholesterol. 2012.
A. Santoso, T. Heriansyah, and M. S. Rohman, “Phospholipase A2 is an Inflammatory Predictor in Cardiovascular Diseases: Is there any Spacious Room to Prove the Causation?,” Curr. Cardiol. Rev. May 2019; 16(1): 3–10.
S Murwani, M Ali, K Muliartha. “DIET ATEROGENIK PADA TIKUS PUTIH (Rattus novergicus strain Wistar) SEBAGAI MODEL HEWAN ATEROSKLEROSIS,” J. Kedokt. Brawijaya. Apr 2013; 22(1): 6–9.
Y Lee et al. Animal models of atherosclerosis (Review) Biomedical Reports, vol. 6, no. 3. Spandidos Publications. 01 Mar 2017; 259–266.
X Li, Y Liu, H Zhang, L Ren, Q Li, N Li. “Animal models for the atherosclerosis research: A review,” Protein and Cell, vol. 2, no. 3. Higher Education Press. 2011; 189–201.
Libby P, Ridker PM, Hansson GK. Inflammation in Atherosclerosis. From Pathophysiology to Practice. Vol. 54, Journal of the American College of Cardiology. 2009. p. 2129–38.
Kusumawati D. Bersahabat dengan Hewan Coba. Yogyakarta: UGM Press; 2004. 65 p.
Vergnes L, Phan J, Strauss M, Tafuri S, Reue K. Cholesterol and Cholate Components of an Atherogenic Diet Induce Distinct Stages of Hepatic Inflammatory Gene Expression. J Biol Chem [Internet]. 2003 Oct 31 [cited 2021 Feb 2];278(44):42774–84. Available from: https://pubmed.ncbi.nlm.nih.gov/12923166/
Ukachukwu UG, Ozougwu VEO, Nwankwo N. A Comparative Study on the Total Cholesterol, Triacylglycerides and Lipid Concentrations of Quail and Chicken Eggs [Internet]. International Journal of Research in Pharmacy and Bioscience. 2017 [cited 2021 Feb 3]. p. 11–6. Available from: https://www.researchgate.net/publication/321426391_A_Comparative_Study_on_the_Total_Cholesterol_Triacylglycerides_and_Lipid_Concentrations_of_Quail_and_Chicken_Eggs
Stewart JW, Kaplan ML, Beitz DC. Pork with a high content of polyunsaturated fatty acids lowers LDL cholesterol in women. Am J Clin Nutr [Internet]. 2001 Aug 1 [cited 2021 Feb 3];74(2):179–87. Available from: https://academic.oup.com/ajcn/article/74/2/179/4739580
Mensink RP. Effects of saturated fatty acids on serum lipids and lipoproteins: a systematic review and regression analysis.
Marques AM, Linhares BS, Dias Novaes R, Freitas MB, Sarandy MM, Gonçalves RV. Effects of the amount and type of carbohydrates used in type 2 diabetes diets in animal models: A systematic review. Fam B, editor. PLoS One [Internet]. 2020 Jun 12 [cited 2021 Feb 3];15(6):e0233364. Available from: https://dx.plos.org/10.1371/journal.pone.0233364
Li X, Liu Y, Zhang H, Ren L, Li Q, Li N. Animal models for the atherosclerosis research: a review.
Volobueva A, Zhang D, Grechko A V., Orekhov AN. Foam cell formation and cholesterol trafficking and metabolism disturbances in atherosclerosis. Vol. 61, Cor et Vasa. Czech Society of Cardiology Z.S; 2019. p. E48–54.
Yu XH, Fu YC, Zhang DW, Yin K, Tang CK. Foam cells in atherosclerosis. Vol. 424, Clinica Chimica Acta. Elsevier; 2013. p. 245–52.
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