Modification of atherogenic diet causes atherosclerosis, increase total cholesterol, and showing hepar damage in mice as alternative animal model in atherosclerosis research

Dzul Fithria Mumtazah; Hendri Busman; Gina Dania Pratami; Ahad Putra Dewantara; Faradhila Amanda

doi:10.30574/gscbps.2021.14.2.0402

Authors

Dzul Fithria Mumtazah Department of Biology, Faculty of Mathematics & Natural Sciences, Universitas Lampung, Jalan Prof. Soemantri Brodjonegoro No. 1 Gedong Meneng, Lampung, Indonesia.
Hendri Busman Department of Biology, Faculty of Mathematics & Natural Sciences, Universitas Lampung, Jalan Prof. Soemantri Brodjonegoro No. 1 Gedong Meneng, Lampung, Indonesia.
Gina Dania Pratami Department of Biology, Faculty of Mathematics & Natural Sciences, Universitas Lampung, Jalan Prof. Soemantri Brodjonegoro No. 1 Gedong Meneng, Lampung, Indonesia.
Ahad Putra Dewantara Department of Biology, Faculty of Mathematics & Natural Sciences, Universitas Lampung, Jalan Prof. Soemantri Brodjonegoro No. 1 Gedong Meneng, Lampung, Indonesia.
Faradhila Amanda Department of Biology, Faculty of Mathematics & Natural Sciences, Universitas Lampung, Jalan Prof. Soemantri Brodjonegoro No. 1 Gedong Meneng, Lampung, Indonesia.

DOI:

https://doi.org/10.30574/gscbps.2021.14.2.0402

Keywords:

Modified atherogenic diet, Atherosclerosis, Foam cell, Total cholesterol, Mice, Hepar damage

Abstract

This study aims to determine the composition of the ideal atherogenic diet to increase the risk of atherosclerosis in the animal model. The research was used 15 male mice, acclimatized for 1-2 weeks to get the susceptible amount of diet to be given. The animal objects are divided into 3 groups; normal diet group (control), 4 weeks atherogenic diet groups, and 8 weeks atherogenic diet groups. The modified atherogenic diet consists of common broiler food, wheat flour, pork oil, quail egg yolks, and water, given 2 times a day of 30 g of food. Water for animal objects is given ad libitum. After 4 weeks and 8 weeks total cholesterol, the formation of foam cells, and hepatocyte degeneration. The results show that the total blood cholesterol of animal models in the group of 8 weeks has the highest level (153, 66 ± 6, 51), compared to other groups (normal diet and 4 weeks). Animal models show that aortic cross-section formed foam cells in tunica intima and tunica media of endotel, also show the indication of hepar damage by hepatocyte degeneration.

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