Isolation and optimization of the fermentation condition of cellulolytic microbial isolates from cassava waste water

Yaya A. Gimba; Abubakar Idris; Abdullahi Hassan; Opeyemi N. Hassan

doi:10.30574/gscbps.2021.14.1.0421

Authors

Yaya A. Gimba Department of Biological Sciences, Niger State Polytechnic Zungeru, Nigeria
Abubakar Idris Department of Biological Sciences, Niger State Polytechnic Zungeru, Nigeria
Abdullahi Hassan Department of Biological Sciences, Niger State Polytechnic Zungeru, Nigeria
Opeyemi N. Hassan Centre International Universitaire Des Meilleurs (C.I.U.M), Bestower International University Seme-Podji-Republique Du Benin.

DOI:

https://doi.org/10.30574/gscbps.2021.14.1.0421

Keywords:

Cassava waste water, Microbial isolate, Cellulase, Optimization

Abstract

The study was conducted to isolate and identify cellulose producing microorganisms from cassava waste water. Cassava waste water sample was obtained from a cassava processing factory at Lokogoma town in Wushishi Local government area of Niger State. The microorganisms were isolated, identified and counted by standard microbiological methods. The mean bacteria count ranges from 6.8 x 10³ cfu/mL^-1to 2.1 x 10³ cfu/mL^-1while the fungi count ranges from 3.2 x 10³ cfu/mL^-1 to 1.2 x 10³ cfu/mL^-1. A total of eight (8) bacterial; Staphylococcus aureus, Bacillus anthrax, Bacillus subtilis, Escherichia coli, Klebsilla sp, Bacillus megaterus, Staph. Epidermidis and Pseudomonas aeruginosa, and six (6) fungi; Saccharomyce serivicea, Aspergillus niger, Penecillium sp., Muccor sp., Aspergilus flavus and Aspergilus fumigetus isolates were identified in the waste water. Among these organism, the best cellulase activity was recorded for Bacillus subtilis (10.39 x 10^-4 mg/ml/sec) and Aspergillus niger (11.21 x 10^-4 mg/ml/sec). However, maximum activity was obtained at pH ranges from 3 ~ 9, temperature ranges from 30^oC ~ 80^oC and substrate concentrations ranges from 1.5% ~3.0%. In conclusion, cassava processing water regarded as waste water could be an alternative source of microorganisms capable of producing cellulase enzyme for industrial purposes.

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