Isolation and optimization of the fermentation condition of cellulolytic microbial isolates from cassava waste water
DOI:
https://doi.org/10.30574/gscbps.2021.14.1.0421Keywords:
Cassava waste water, Microbial isolate, Cellulase, OptimizationAbstract
The study was conducted to isolate and identify cellulose producing microorganisms from cassava waste water. Cassava waste water sample was obtained from a cassava processing factory at Lokogoma town in Wushishi Local government area of Niger State. The microorganisms were isolated, identified and counted by standard microbiological methods. The mean bacteria count ranges from 6.8 x 103 cfu/mL-1 to 2.1 x 103 cfu/mL-1 while the fungi count ranges from 3.2 x 103 cfu/mL-1 to 1.2 x 103 cfu/mL-1. A total of eight (8) bacterial; Staphylococcus aureus, Bacillus anthrax, Bacillus subtilis, Escherichia coli, Klebsilla sp, Bacillus megaterus, Staph. Epidermidis and Pseudomonas aeruginosa, and six (6) fungi; Saccharomyce serivicea, Aspergillus niger, Penecillium sp., Muccor sp., Aspergilus flavus and Aspergilus fumigetus isolates were identified in the waste water. Among these organism, the best cellulase activity was recorded for Bacillus subtilis (10.39 x 10-4 mg/ml/sec) and Aspergillus niger (11.21 x 10-4 mg/ml/sec). However, maximum activity was obtained at pH ranges from 3 ~ 9, temperature ranges from 30 oC ~ 80oC and substrate concentrations ranges from 1.5% ~3.0%. In conclusion, cassava processing water regarded as waste water could be an alternative source of microorganisms capable of producing cellulase enzyme for industrial purposes.
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References
Subramaniyam R, Vimala R. Solid state and submerged fermentation for the production of bioactive substances: A comparative study. Int J Sci Nature. 2012; 3(3): 480-486.
Zhang ZJ, Chen YZ, Hu HR, Sang YZ. The beatability-aiding effectof Aspergillus niger crude cellulase onbleached simao pine Kraft pulp and itsmechanism of action. BioResources. 2013; 8: 5861-5870.
Korpole S, Sharma R, Verma D. Characterization and phylogenetic diversity ofcarboxymethyl cellulase producing Bacillus species from a landfill ecosystem.Indian J Microbiol. 2011; 51(4): 531-535.
Deka D, Bhargav P, Sharma A, Goyal D, Jawed M, Goyal A. (2011). Enhancement ofcellulase activity from a new strain of Bacillus subtilis by medium optimization andanalysis with various cellulosic substrates. Enzyme Res. 2011; 2011: 151656.
Kasana RC, Salawan R, Dhar H, Dutt S, Gulati A. A rapid and easy method for thedetection of microbial celluloses on agar plates using gram’s iodine. Curr Microbiol. 2008; 57(5): 53-507.
Knauf M, Moniruzzaman M. Lignocellulosic biomass processing: a perspective. Int Sugar J. 2014; 106: 147–50.
Iyayi EA. Losel DM. Changes incarbohydrate fractions of cassava peelsfollowing fungal solid state fermentation.Journal of Food Technology, Africa. 2011; 6: 101-103.
Arotupin DJ. Evaluation of Microorganisms from Cassava Wastewater for Production of Amylase and Cellulase. Research Journal ofMicrobiology. 2017; 2: 475-480.
Holt JG, Kneg NR, Sneath PH, Stanly JJ, Williams ST. Bergeys manual of determinative bacteriology, Wilkins Publishers, Baltimore. 1994; 783.
Barnett HL, Hunter BB. Illustrated Genera of Imperfect Fungi, 3rd Edn. Burges Publishing Company, Minneapolis. 1972; 331.
Cowan ST, Steel KJ, Shaw C. A classification of the Klebsiella group. J Gen Microbiol. 1960; 23: 601–612.
Olowoyo, O. O, Adetuyi, F. C and Oshodi, A. A., Microbial analysis of stored flour. Appl. Nat. Sci. Res. 2001;1:3.
Akinyosoye, F.A., A.H. Adeniran and G. Oboh, 2003. Production of fungal amylase from agro-industrial wastes. Proceedings of the 16th Annual Conference of Biotechnology Society of Nigeria, September 8-11, 2003, Federal University of Technology, Akure, Nigeria, pp: 35-42.
Uzochukwu S, Balogh E, Loefler RT, Ngoddy PO. Structural analysis by 13 C-nuclear magnetic resonance spectroscopy of glucans elaborated by gum-producing bacteria isolated from palm wine. Food Chem. 2001; 73: 225–233.
Saravanan p, MuthuvelayudhamR, Viruthagiri T. Enhancedproduction of cellulose from pinewaste by response surfacemethodogology.,J. of Engineering. 2013; 8.
Peij N, Gielkens MMC, Verles RP, Visser K, Graff LH. Thetranscriptional activator Xin Rregulates both xylanolyticendoglucanase gene expressions inAspergillus niger. Applied Environ.Microbiol. 2008; 64: 3615-3617.
Xia L, Cen P. Cellulase productionby solid state fermentation onlignocellulosic waste from the xyloseindustry.Process Biochemistry. 2009; 34: 909-912.
Riley D. Simple repeat replacementssupport similar functions of distinctrepeats in. 2014.
Hebeish A, Ibrahim NA. The impact of frontier sciences on textile industry.Colourage. 2007; 54: 41-55.
Ikram-Ul-Haq, Uzma Hameed, KiranShahzadi,M., Mohin Javed, Sikander Aliand Qadeer MA. Cottonsaccharifying activity of cellulases byTrichoderma harzianum UM-11 inshake flask. Intl. J. Bot. 2015; 1(1): 19-22.
Omajasola P, Folakemi, Jilani, Omowumi Priscilla, Ibiyemi SA. Cellulase production by somefungi cultured on pine apple waste.Nature and Science. 2008; 6(2): ISSN : 1545-0740.
Oboh G. Nutrient enrichment of cassavapeels using a mixed culture of Saccharomyces cerevisae andLactobacillus spp. Solid mediafermentation techniques.ElectronicJournal of Biotechnology. 2016; 9(1).
O'sullivan AC. Cellulose: Thestructure slowly unravels. Cellulose. 2007; 4: 173-207.
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