Phytochemical analysis and in vitro screening of antifungal activity of Jatropha multifida, Euphorbia hirta, Occimum gratissimum and Mitracarpus scaber leaves extract

Nwofor Chioma N; Oyeka Christie A; Onyenwe Nathaniel E; Fajana Aqib

doi:10.30574/gscbps.2021.14.3.0023

Authors

Nwofor Chioma N Department of applied microbiology and brewing Nnamdi Azikiwe University Awka Nigeria.
Oyeka Christie A Department of applied microbiology and brewing Nnamdi Azikiwe University Awka Nigeria.
Onyenwe Nathaniel E Department of Pharmaceutical Microbiology, College of Pharmacy, Igbenedion University Okada, Benin Nigeria.
Fajana Aqib Department of Pharmacognosy, College of Pharmacy, Igbenedion University Okada, Benin Nigeria.

DOI:

https://doi.org/10.30574/gscbps.2021.14.3.0023

Keywords:

Phytochemicals, Jatropha multifida, Euphorbia hirta, Occimum gratissimum and Mitracarpus scaber

Abstract

The research study was to understudy the qualitative and quantitative phytochemical compounds and antifungal activity of methanolic extracts of Jatropha multifida, Euphorbia hirta, Occimum gratissimum and Mitracarpus scaber leaves against 12 species of fungi.

The 8 phytochemical compounds tested were saponin, phenol, tannin, flavonoids, steroids, terpenoid, alkaloid and glycoside. The antifungi sensitivity test was analysed by Kirby-bauer disc diffusion method. The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) was analysed by broth tube dilution method followed by subculturing the relative samples. The plant extracts were prepared in the concentration range of 6.25, 12.5, 25, 50,100 and 200 mg/ml. The antifungal sensitivity test showed that Jatropha multifida (200mg/ml) was weakly effective against Fusarium solani and resistant against others. Occimum gratissimum, Euphorbia hirta and Mitracarpus scaber at (200mg/ml) respectively was highly effective on most of the fungi species tested with zone of inhibition of 10-20mm. Jatropha multifida had MIC and MFC (>200mg/ml), Occimum gratissimum had MIC and MFC (100 and >100mg/ml), Euphorbia hirta (25-200 and 50 - >200mg/ml) and Mitracarpus scaber had (25 - 100 and 50-200mg/ml) on most test isolates respectively. Investigations showed, Mitracarpus scaber were highly effective, followed by Euphorbia hirta, Occimum gratissimum and Jatropha multifida on the test organisms and could be attributed to the presence of phytochemical compounds and as such can be harnessed and used as a source of alternative medicine especially in the tropics like the South-eastern Nigeria.

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References

Gini TG, Jothi Jeya G. In vitro screening of antibacterial and antifungal activity of Marsileaquadrifolia (Marsileaceae) Linn. Extract. American Journal of Phytomedicine and Therapeutics. 2015; 3(4): 313 – 329.

Falodun A. Herbal medicine in Africa-distribution, standardization and prospects. Research Journal of Phytochemistry. 2010; 4(3): 154-161.

Amir Muhammad Khan, Rizwana Aleem Qureshi, Faizan Ullah, Syed Aneel Gilani, Asia Nosheen, Sumaira Sahreen, Muhammad Khan Laghari, Shafia-Ur- Rehman, Ishtiag Hussain, Waheed Murad. Phytochemical analysis of medicinal plants of Margalla Hills and surroundings. Journal of Medicinalplants Research. 2011; 5(25): 6017-6023.

Jachak SM, Saklani A. Challenges and opportunities in drug discovery from plants. Journal of current science. 2007; 92(1): 1251-125.

Michael Niyi Aransiola, Charele Ehikhase, Joy C. Mmegwa, Idris Olayinka Wahab. Microbiology: A systems approach by Majorie Kelly Cowan and Kathleen Park Talaro.Published by Mc Graw Hill.1st Edition Newyork,America. 2006; 131-139.

Abere TA, Onyekweli AO, Ukoh GC. In vitro Antimicrobial activity of the extract of Mitracarpus Scaber leaves formulated as syrup. Tropical Journal of Pharmaceutical Research. 2007; 6(1): 679-682.

Hemandez NE, Tereschuk ML, Abdala LR. Antimicrobial activity of flavonoid in medicinal plants from tatidel valle (Tacuman, Argentina). Journal of Ethanopharmacol. 2000; 73(1-2): 317-322.

Karaye P. Gloria, Dogo GI. Abraham, Karaye Kuduk. Evaluation of antimycotic activity of crude methanolic extract of mitrocarpus scaber on candida albicans and Trichophyton mentagrophytes. Saudi Journal of medical and pharmaceutical Sciences. 2017; 3(3): 217-220.

Anejionu Miriam Goodness, Nweze Emeka Innocent, Dibua Esther Uju, Esimone Charles Okechukwu. Efficacy of two commonly used antifungal Herbs in Nigeria against Clinical isolates of Fungi. Microbiology Journal. 2012; 2(3): 70-8.

Thes PM, Soumahoro IA, Ackah JA, Zirihi GN, Djaman AJ. Action du savon misca-mates dans le trairement des teignes. Phytotherapie. 2011; 9(6): 354 – 358.

Kumar A, Sharma S. An evaluation of multipurpose of seed crop for industrial uses (Jatropha curcas). A review Bios Eng . 2008; 97: 201-207.

Martinez-Herrara J, Siddhuraju P, Francis G, Davila-Ortiz G, Becker K. Chemical composition toxic/antimetabolic constituents and effects of different treatments on their levels in four provenances of Jatropha curcas L. from Mexico. Food Chem. 2006; 96: 80-89.

Reddy Prasad DM., Amirah Izam, Maksudur Rahman Khan Md. Jatropha curcas: plants ofmedicinal benefits. Journal of Medicinal Plants Research. 2012; 6(14): 2691 – 2699.

Rakshit K Devapapa, Harinder PS Makkar, Klaus Becker. Jatropha toxicity - A Review Journal of Toxicology and Environmental Health. 2010; 13(6): 476-507.

Jati Batoro, Dian Siswanto. Ethno Medicina; survey of plants used by local society in Poncokusumo district Malang, East Java Province, Indonesia, Asian Journal Medical Biological Research . 2017; 3(2): 158-167.

Wongsatit Chuakul. Medicinal plants in the Khok Pho District, Pattani Province (Thailand) The Journal of Phytopharmacy. 2005; 12(2): 23-45.

Olapeju Aiyelagbe, Bola J. Oguntuase, Babatunde D. Arimah, Bolanle A. Adeniyi. The antimicrobial activity of Jatropha multifida extracts and chromatographic fractions against sexually transmitted infections. Journal of Medical Sciences (Faisalabad). 2008; 8(2): 143-147.

Matasyoh LG, JC Matasyoh, FN wachira, MG Kinyua, AW Thairu Muigai, TK Mukiama. Chemical composition and antimicrobial activity of the essential oil of Ocimum grastissimum leaves groeing in Eastern Kenya. African Journal of Biotechnology. 2007; 6(6): 760-765.

Koche DK, Kokate PS, Suradkar SS and Bhadange DG. Prelimanry Phytochemistry and Antibacterial Activity of ethanolic Extract of Ocimum Gratissimum Leaves. Bioscience Discovery. 2012; 3(1): 20-24.

Mbakwem-Aniebo C, Onianwa O, Okonko IO. Effects of Ocimum Gratissimum Leaves on common dermatophytes and causative agent of pityriasis versicolor in Rivers State, Nigeria. Journal of Microbiology Research. 2012; 2(4): 108-113.

Nwinyi OC, Chinedu NS, Ajani OO, Ikpo CO, Ogunniran KO. Antibacterial effects of extracts of Ocimum gratissimum and piper guineense on Escherichia coli and Staphylococcus aureus. African Journal of Food Science. 2009; 3(3): 077-081.

Nweze EL, Eze EE. Justification for the use of Ocimum gratissimum in herbal medicine and its interaction with disc antibiotics. BMC complementary and Alternative medicine. 2009; 9(37): 1-6.

Ijeh II, Njoku OU, Ekenza EC. Medicinal Evaluation of xylopia aethiopica and Ocimum gratissimum. Journal of medicinal Aromatis Science. 2004; 26(1): 44-48.

Ladipo MK, Doherty VF, Kanife UC. Phytochemical Screening and antibacterial investigation of the extract of Ocimum gratissimum (Scent Leaf) on selected Enterobacteriaceae. Production agriculture and technology. 2010; 6(2): 75-84.

Silva MRR, Oliveria Jr, Fernandes OFL, Passos XS, Costa CR, Souza LKH, Lemos JA, Paula JR. Antifungal activity of Occimum gratissimum towards dermatophytes. Mycoses. 2005; 48(3): 172 – 175.

Chitra M, Muga V, Sasikumar Dhanarasu, Awdah Masoud Al-hazimi. Screening of phytochemical and In-vitro activity of Euphorbia hirta L. Journal of chemical pharmaceutical Research. 2011; 3(6): 110-114.

Juveriyah Kausar, Durai Muthumani, Agaath Hedina, Sivasamy, Vijaya Anand. Review of the phytochemical and pharmacological activities of Euphorbia hirta Linn. Pharmacognosy Journal. 2016; 8(4): 310-313.

Huang Linfang, Chen Shillin, Yang Melhua. Euphorbia hirta (Feiyangcao): A review on its ethnopharmacology, phytochemistry and pharmacology. Journal of Medicinal Plants Research. 2012; 6(39): 5176-5185.

Saravanan R, Dhachinamoorthi D, Senthilkumar K, Srilakshmi M, Sri Divya T. Antibacterial activity of Euphorbia hirta extracts. International Journal of Research in Ayurveda and Pharmacy. 2012; 3(3): 439-441.

Rajah Mohammad Abu Basma, Zuraini Zakaria, Sasidharan Sreenivasan, Latha Lachimanan. Assessment of Euphobia hirta L. Leaf, Flower, stem and Root Extract for their antibacterial and antifungal activity and Brine shrimp lethality. Molecules. 2010; 15: 6008-6018.

Singh Geeta, Kumar Padma. Extraction and antimicrobial activity of alkaloids of Euphorbia hirta. Journal of Pharmacy Research. 2011; 4(4): 1228-1230.

Tabassum Nahida, Hamdani Mariya. Plants used to treat skin diseases. Pharmacognosy Review. 2014; 8(15): 52-60.

Mbata TI, Duru MC, Onwumelu HA. Antibacterial activity of crude seed extracts of Buchholzia coriacea E. on some pathogenic bacteria. Journal of Dev.Bio and Tissue Engr. 2009; 1(1): 001-005.

Ameh GI. Evaluation of the phytochemical composition and antibacterial properties of crude methanolic extract of leaves of Ocimum gratissimum. J. Nat. Sci. Engr. Tech. 2010; 9(1): 147-152.

Harbornen JB. Phytochemical methods: A guide to modern technique in plant analysis.(3rd ed). London: Chapman and Hall. 1998.

Khandelwal KR. Pratical pharmacognosy, Techniques and Experimentals.16th Edn.,Nirali Prakashan,Pune,India,ISBN:81-85790-30-2. 2006; 107.

Sofowora A. Medicinal plants and Traditional Medicinal in Africa,2nd Ed. Sunshine House, Ibadan,Nigeria: Spectrum Books Ltd: screening plants for Bioactive Agents. 1993; 134-156.

Trease GE, Evans WC. Pharmacology (12th ed). Bailliere Tindal:London. 1983.

Harborne JB. Methods of plant analysis. In phytochemical Methods Chapman and Hall, London.1973.

Bohn BA, Kocipai- Abyazan R. Flavonoid and condensed tannins from the leaves of vaccinum raticulation and Vaccinum calcyimium. Pacific Science. 1994; 48: 458 -463.

Polshettiwar SA, Ganjiwale RO, Wadher SJ, Yeole PG. Spectrophotmetric estimation of total tannins in some Ayurvedic eye drops. Indian Journal of pharmacological Science. 2007; 69: 574 -576.

Clinical and laboratory Standard Institute (2008). Specimen handling and processing Speciality Collection (CLSI.).

Geeta Singh and Padma Kumar. Phytochemical study and screening for antimicrobial activity of flavonoids of Euphorbia hirta. International journal of applied basic medicinal research. 2013; 3(2): 111-116.

Afolabi Clement Akinmoladun, Emmanuel Afor Efere Martins Obuofor. Phytochemical constituent and antioxidant activity of extract from leaves of Occimum grastissium. Scientific research and essays. 2007; 2(5): 163-166.

Gupta VK, Singh J, Kumar R, Bhanot A. Pharmacognostic and preliminary phytochemical study of Occimum gratissium Linn ( Family;lamiaceae). Asian journal of plant sciences. 2011; 10: 365-369.

Pricilla, Alexander. Phytochemical screening and mineral composition of the leaves of Occimum grastissium (scent leaves). International journal of applied sciences and biotechnology. 2016; 4(2): 161-165.

Orji JO, Nwuzo AC, Ejikeugwu PC, Ugdo EN, Moses IB, Nwakaeze EA, Nwankwo CP. Antifungal activities of Occimum gratissimum and Gongronema latifolium leaves on colletotrichym species isolated from spoilt tomatoes. International Journal of Pharmaceutical Science invention. 2015; 4(5): 42 – 45.

Chilaka KC, Unekwe PC, Enyi JC, Ogamba JO. Evaluation of antimicrobial properties of methanol extract of Mitracarpus scaber leaves. Tropical journal of medical research. 2009; 13(2): 10-15.

Ouadja B, Anani K, Djrei B, Ameyapoh YO, Karou DS. Evaluation of the phytochemical composition, antimicrobial and antiradical activites of Mitracarpus scaber (Rubiaceae). Journal of medicinal plants Research. 2018; 12(28): 493-499.

Shinkafi SA. Antidermatophytic activities, phytochemical screening and chromatographic studies of Pergularia tomentosa L. and Mitracarpus scaber zucc.( Leaves) used in the treatment of dermatophytoses. International Journal of Microbiology. 2013; 4(1): 29-37.

Nwokocha A, Blessing IO. Agbagwa, BE Okoli. Comparative phytochemical screening of Jatropha L.species in the Niger Delta. Researh journal of phytochemistry. 2011; 5: 107-114.

Sallykutty Thomas. Pharmacognostic and phytochemical constituents of leaves of Jatropha multifida Linn and Jatropha podagrica Hook. Journal of pharmacognosy and phytochemistry. 2016; 5(2): 243-246.

El-Mewafy Abdou, El Mewafy El Ghadban, Omneya Farouk Abou El- leel, Ehab MB Mahdy. Morphological phytochemical and molecular characterization on some Jatropha species cultivated in Egypt. International Journal of Pharmaceutical Science and Scientific Research. 2016; 3(1): 1-13.

Nouioua Wafa, Gaamoune Sofiane, Kaabache Mouhamed. The antioxidant and antimicrobial activities of flavonoids and tannins extracted from Phlomis bovei De Noe. European Journal of Experimental Biology. 2016; 6(3): 55-61.

Sillma Rampadarath, Daneshwar Puchooa and Mala Ranghoo Sanmukhiya. Antimicrobial, phytochemical and insecticidial properties of Jatropha species and Wild Ricinus Commnunis I found in Mauritius. International Journal of Pharmacognosy and Phytochemical Research. 2014; 6(4): 831 – 840.

Adekunle AA, Uma UN. Effect of benlate solution,crude leaf extract of Azadirachta indica and Occimum gratissimum on growth of fungi and preservation of melon seeds. Plant pathology Journal. 2005; 4(1): 29 -34.

Waseem Ahmad, Shilpa Singh, Sanjay Kumar. Phytochemical screening and antimicrobial study of Euphorbia hirta extracts. Journal of medicinal plants studies. 2017; 5(2): 183 – 186.

Ilondu EM, Bosah BO. Growth inhibition potentials of leaves extract from four selected Euphorbiaceae against fruit rot fungi of Africa star apple (Chrysophyllum albidun GDon). Journal plant pathology and microbiology. 2015; 6(9): 1 -6.

Cimanga RK, Kambu KL, Tona Bruyne, Dea T, Sandara A, Jotte T, Pietera L, Vlietinck AJ. Antibacterial and antifungal activities of some extracts and fractions of Mitracarpus scaber zucc (Rubiaceae). Journal of natural remedies. 2004; 4(1): 17 – 25.