Antimicrobial susceptibility of H. pylori isolated from suspected patient using stool sample at Malali Hospital and Maternity Clinic Kaduna, Kaduna State – Nigeria

Authors

  • Rabilu Sani Department of Community Health Science, Shehu Idris Collage of Health Sciences and Technology, Makarfi, Kaduna State, Kaduna – Nigeria.

DOI:

https://doi.org/10.30574/gscbps.2021.14.3.0042

Keywords:

Antimicrobial, susceptibility, Helicobacter pylori, Patient, Stool, Microaerobic, Dilution and centrifugation

Abstract

The antimicrobial susceptibility of Helicobacter pylori isolated from suspected patient using stool sample at Beijing greatest college of health sciences and technology, Kaduna, demonstration clinic.

The analyses were carried out to further understand the intestinal environment condition of the sample sites. As it has been noted that: it is difficult of growing Helicobacter pylori in a broth, hence, no specific enrichment has been proposed.

Note: As everything present in the stomach would be found in the stools, hence there is no doubt that Helicobacter pylori can be eliminated via this route and successful culturing of Helicobacter pylori from the stool sample had been achieved. [4]. The stool was diluted to a 20% w/v solution of in phosphate buffered saline (PBS) and the suspension was sieved through a 250 um strainer before plating onto the selective media. The sample was passed through a series of dilution techniques and centrifugation before finally plating on to the deoxycholate citrate agar (selective) and incubates at 37oC under the microacrobic atmospheric oxygen concentration of 7 – 12% (O2) for four (4) days.

Across the cultured, the cultured plate there was marked abundant of bacterial cells with some colonies having the characteristics of Helicobacter pylori which later sub-cultured and isolated for antimicrobial test.

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References

Argyros FC, Ghosh M, Huang L, et al. Evaluation of a PCR primer based on the isocitrate dehydrogenase gene for the detection of Helicobacter pylori infeces. J Clin Microbiol. 2000; 38: 3755–58.

Mapstone NP. PCR identification of Helicobacter pylori in faeces from gastritis patients. Lancet. 1993; 341: 447.

Gramley WA, Asghar A, Frierson HF JP, et al. Detection of Helicobacter pylori DNA in faecal samples from infected individuals. J Clin Microbiol. 1999; 37: 2236–40.

BSAC Working Party. Recommendations for susceptibility tests on fastidious organisms and those requiring special handling. J Antimicrob Chemother. 2001; 48 (suppl S1): 77–80.

Megraud F, Hazell S, Glupczynski Y. Antibiotic susceptibility and resistance. In: Mobley HLT, Mendz GL, Hazell SL eds. Helicobacter pylori: physiology and genetics. Washington DC: ASM Press. 2001: 511–30.

Isenberg RD. Clinical Microbiology Procedures Handbook 2’ Edition.

Jorgensen JH, Pfaller MA, Catroll KC, Funke G, Landrv ML, Richter SS, Wamock DW. Manual of Clinical Microbiology. 2015.

Salfïnger Y, Tortorello ML. Compendium of Methods for the Microbiological Examination of Foods. 5th Ed. American Public Health Association. Washington. D.C. 2015.

Rupprecht HJ, Blankenberg S, Bickel C, et al. Impact of viral and bacterial infectious burden on long-term prognosis in patients with coronary artery disease. Circulation. 2001;104:25–31.

Pietroiusti A, Diomedi M, Silvestrini M, et al. Cytotoxin-associated gene-A-positive Helicobacter pylori strains are associated with atherosclerotic stroke. Circulation. 2002;106:580–4.

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Published

2021-03-30

How to Cite

Rabilu Sani. (2021). Antimicrobial susceptibility of H. pylori isolated from suspected patient using stool sample at Malali Hospital and Maternity Clinic Kaduna, Kaduna State – Nigeria. GSC Biological and Pharmaceutical Sciences, 14(3), 122–128. https://doi.org/10.30574/gscbps.2021.14.3.0042

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Original Article