Hepatotoxicity effects of Methanol extract of Gmelina arborea leaves on liver enzymes marker in Wistar Rats

Abstract

Liver diseases are a major cause of mortality and morbidity in developing countries. This study aims at determining the hepatotoxicity effects of methanolic-extracts of Gmelina arborea leaves at different concentrations on liver marker enzymes in wistar rats. The experiment was laid out in a complete randomized design (CRD). Fresh leaves of Gmelina arborea from the school environments was collected, dried, grinded and extracted using muslin cloth and methanol. The extract was then placed in the oven at about 38^oC for dryness. The extract was administered to five groups of wistar rats orally and twice daily for 21 days at doses of 1000mg/kg, 500mg/kg, 250mg/kg, 125mg/kg and group one control group (wasn’t administered this extract). At the end of every week (7 days) the blood samples were collected through ocular puncture, and biochemical analysis of Serum test (Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), Alkaline phosphatase (ALP) , Total bilirubin (TB) and Conjugated bilirubin (CB) were estimated. There was no mortality observed as a result of administration of this leave extract. Methanol extract of Gmelina arborea did not produce significant differences in the body weight, organ weights and feeding habits between control and treated animals. Pathologically, neither gross abnormalities nor histopathological changes were observed. Methanol extract of Gmelina arborea was found safe in acute and repeated dose toxicity study when tested in wistar rat.