Antibacterial activity of Piper betle ethanolic extract against ESBL producing Escherichia coli in sepsis induced BALB/c mice

Shod Abdurrachman  Dzulkarnain; Irene  Ratridewi; Vanianissa Hanareta  Hantoko; Yessy Chairani Puspita  Sari; Dewi  Santosaningsih

doi:10.30574/gscbps.2021.15.2.0113

Authors

Shod Abdurrachman Dzulkarnain Faculty of Medicine, Brawijaya University, Malang, East Java, 65145, Indonesia.
Irene Ratridewi Department of Pediatrics of Saiful Anwar General Hospital, Faculty of Medicine, Brawijaya University, Malang, East Java, 65145, Indonesia.
Vanianissa Hanareta Hantoko Faculty of Medicine, Brawijaya University, Malang, East Java, 65145, Indonesia.
Yessy Chairani Puspita Sari Faculty of Medicine, Brawijaya University, Malang, East Java, 65145, Indonesia.
Dewi Santosaningsih Department of Clinical Microbiology of Saiful Anwar General Hospital, Faculty of Medicine, Brawijaya University, Malang, East Java, 65145, Indonesia.

DOI:

https://doi.org/10.30574/gscbps.2021.15.2.0113

Keywords:

Piper betle ethanolic extract, ESBL producing Escherichia coli, Sepsis induced mice, Survival rate

Abstract

Sepsis is an organ dysfunction caused by dysregulation of immune response towards systemic infection with high incidence and mortality rate. Innovative breakthrough is required to manage sepsis especially extended spectrum beta lactamase (ESBL) producing Escherichia coli (E. coli) induced sepsis. Utilization of Piper betle extract to manage sepsis could be considered as an alternative therapy and should be proven scientifically so meropenem utilization could be reduced. This study aimed to evaluate the antimicrobial effect of Piper betle ethanolic extract in BALB/c mice infected with ESBL producing E. coli. We identified terpene, apiol, polyphenol, eugenol, myristicin, flavonoid, safrole, lynalil acetate, and allyl tetramethoxy benzene in the Piper betle ethanolic extract using thin layer chromatography. The minimum inhibitory concentration of the Piper betle ethanolic extract against ESBL producing E. coli was 3 mg/mL assessed using broth dilution method. We evaluated the effect of Piper betle ethanolic extract to the survival rate of BALB/c mice infected with ESBL producing E. coli. The single dose administration of Piper betle ethanolic extract prolonged the survival rate of the infected BALB/c mice for 6 hours.

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