Potential exploitation of Shea press cakes in glycaemia regulation: Inhibition of α-amylase and α-glucosidase by protein and methanolic extracts

Silas Elisée Ahouman  Djoman; Boris Abel  Kouakou; Rose-Monde  Mégnanou; Gladys Ginette  Doué

doi:10.30574/gscbps.2021.15.2.0119

Authors

Silas Elisée Ahouman Djoman Biotechnology, Agriculture and Valorisation of Biological Resources Laboratory / UFR Biosciences / University Felix Houphouët Boigny in Abidjan / Côte d’ivoire.
Boris Abel Kouakou Biotechnology, Agriculture and Valorisation of Biological Resources Laboratory / UFR Biosciences / University Felix Houphouët Boigny in Abidjan / Côte d’ivoire.
Rose-Monde Mégnanou Biotechnology, Agriculture and Valorisation of Biological Resources Laboratory / UFR Biosciences / University Felix Houphouët Boigny in Abidjan / Côte d’ivoire.
Gladys Ginette Doué Biotechnology, Agriculture and Valorisation of Biological Resources Laboratory / UFR Biosciences / University Felix Houphouët Boigny in Abidjan / Côte d’ivoire.

DOI:

https://doi.org/10.30574/gscbps.2021.15.2.0119

Keywords:

Shea press cakes, Protein and methanolic extracts, Glycaemia, α-amylase, α-glucosidase

Abstract

Shea (Vitellaria paradoxa) tree is integrally used in traditional medicine for the treatment of several health disturbances. Its kernels fat is widely exploited for food, medicinal and cosmetic purposes. Nevertheless germinated kernels are considered as waste, whereas shea germinative power would be very high. Their anti-diabetic ability was evaluated in vitro, in ordre to highlight their pharmacological benefits. Therefore, both proteins (crude, digested and dialysed ones) and hydroalcoholic extracts, were prepared from germinated and ungerminated shea seed press cakes. The anti-diabetic essay was carried out by evaluating extracts inhibiting power on both α-amylase and α-glucosidase activities. Proteins were quantified by spectrophotometry (214 nm). Results revealed that the protein content of the extracts from germinated seed cakes was 450 mg/100 g and that of the extracts from ungerminated shea seed cakes was 410 mg/100 g. The percentage of inhibition of α-amylase by the dialysed extracts of germinated shea seeds, in this case the external dialysate of germinated seed, presented the best rate of inhibition with 30.21 %. Contrary to the percentage of inhibition of α-amylase, the highest rates of inhibition of α-glucosidase were recorded with the crude protein extracts of sprouted seeds (82.02 %) and unsprouted seeds (62.32 %). For methanolic extracts, the highest inhibition of α-amylase and α-glucosidase was recorded by the ungerminated seeds, with 42.61% for α-amylase and 97.47% for α-glucosidase. These results show that protein extracts of shea seed cakes may play a role in blood glucose regulation.

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References

Rodier M. Définition et classification du diabète. Définition et classification du diabète. Médecine Nucléaire - Imag. Fonct. Métabolique. 2001; 25: 91–93.

Whiting D, Guariguata L, Weil C, Shaw J. IDF diabetes atlas: global estimates of the prevalence of diabetes for 2011 and 2030. Diabetes Res Clin Pr. 2011; 94: 311–21.

Grant PJ. Beneficial effects of metformin on haemostasis and vascular function in man. Diabetes Metab. 2003; 29: 6S44-6S52.

Bailey CJ. Metformin: Effects on micro and macrovascular complications in type 2 diabetes. Cardiovasc. Drugs Ther. 2008; 22: 215–224.

Barkaoui M, Katiri A, Boubaker H, Msanda F. Ethnobotanical survey of medicinal plants used in the traditional treatment of diabetes in Chtouka Ait Baha and Tiznit (Western Anti-Atlas), Morocco. J. Ethnopharmacol. 2017.

Pesquet JJ. Le Karité. APROMA, Revue Bimestrielle. Nos. 27-28-29, Décembre 1992; 8-13.

Hall JB, Aebischer DP, Tomlinson DF, Osei-Amaning E, Dindle JR. Vitellaria paradoxa. A Monograph. School of Agricultural and Forest Sciences. University of Wales Bangor. 1996; 105.

Kouamé IAD. Influence de la germination et de la fermentation des grains de sésame (Sesamun indicum L.) sur la qualité nutritionnelle et fonctionnelle des farines et huiles dérivées. Thèse unique. Université Nangui Abrogoua d’Abidjan Cote d’Ivoire. 2019; 185.

González-Montoya M, Hernández-Ledesma B, Mora-Escobedo R, Martínez-Villaluenga C. Bioactive Peptides from Germinated Soybean with Anti-Diabetic Potential by Inhibition of Dipeptidyl Peptidase-IV, -Amylase, and -Glucosidase Enzymes. International journal of molecular sciences. 2018; 1-14.

Acosta C, Carpio C, Vilcacundo R, Carrillo W. Identification of proteins isolate from amaranth (Amaranthus caudatus) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis with water and NaCl 0.1 m solvents. Asian J. Pharm. Clin. Res. 2016;9(3):331-4.

Gavrilorie M, Maginot MJ, Gavrilorie CS, Wallach J. Manipulation d’analyse biochimique. 3ème édition Doin Paris. 1996; 112-120.

Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods in Enzymology. 1999; 299: 152-178.

Marcela EK, Koehnlein EK, Corrêa RCG, Nishida VS, Correa VG, Bracht V, Peralta RM. Analyse d'un régime alimentaire complet en termes de teneur phénolique et de capacité antioxydante : effets d'une digestion gastro-intestinale simulée. International Journal of Food Sciences and Nutrition. 2016; 67(6): 614-623.

Hashim A, Khan MS, Khan MS, Baig MH, Ahmad S. Antioxidant and α-Amylase Inhibitory Property of Phyllanthus virgatus L.: An In Vitro and Molecular Interaction Study. Journal of Biomedicine and Biotechnology. 2013; 1-12.

Vilcacundo R, Martínez-Villaluenga C, Miralles B, Hernández- Ledesma B. 2018. Release of multifunctional peptides from kiwicha (Amaranthus caudatus) protein under in vitro gastrointestinal digestion., Journal of the Science of Food and Agriculture. 2018; 1-29.

Marze S. Chapter 14 – Modeling of Food Digestion. Modeling of Microscale Transport in Biological Processes. 2017; 353–374.

OMS & Fédération Internationale du Diabète. L’atlas du diabète de la FID. 9ième édition. 176p.le29/11/20, Disponible sur. 2016.

Auvray P, Duval E, Baron A, Chauvin C, Hesry V, Jouquan D, Martin M, Redon E, Sourdaine P, Bourgeon R, Melaine N, Pineau C, Rouillon A, Bosseboeuf A. Peptides isolés et purifiés à partir de testicules de roussettes. Demande internationale publiée en vertu du traité de coopération en matière de brevets. 2012; 48.

Kim Y, Keogh J, Clifton P. Polyphenols and Glycemic Control. Nutrients. 2016; 8: 17.

Martel J, Ojcius DM, Chang CJ, Lin CS, Lu CC, Ko YF, Tseng SF, Lai HC, Young JD. Anti-obesogenic and antidiabetic effets of plant and mushrooms. Nat. Rev. Endocrimal. 2016.

Solayman M, Ali Y, Alam F, Islam A, Alam N, Ibrahim Khalil M, Hua Gan S, Others. Polyphenols: potential future arsenals in the treatment of diabetes. Curr. Pharm. Des. 2016; 22: 549–565.

Funke I, Melzig MF. Effect of different phenolic compounds on alpha-amylase activity: screening by microplate-reader based kinetic assay. Pharm. 2005; 60: 796–797.

McDougall GJ, Stewart D. The inhibitory effects of berry polyphenols on digestive enzymes. BioFactors Oxf. Engl. 2005; 23: 189–195.

Tadera K, Minami Y, Takamatsu K, Matsuoka T. Inhibition of alpha-glucosidase and alpha-amylase by flavonoids. J. Nutr. Sci. Vitaminol. (Tokyo). 2006; 52: 149–153.