Phytochemical analysis, antioxidant and anti-inflammatory activities of Chassalia kolly leaves extract, a plant used in Benin to treat skin illness

Koudoro Yaya  Alain; Awadji Jospin  Morand; Botezatu Dediu  Andreea; Olaye  Théophile; Agbangnan Dossa Cokou  Pascal; Alitonou Guy  Alain; Avlessi  Félicien; Dinica Rodica  Mihaela; Sohounhloue Codjo Koko  Dominique

doi:10.30574/gscbps.2021.15.3.0148

Authors

Koudoro Yaya Alain Laboratory of Study and Research in Applied Chemistry, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, BP 2009 Abomey-Calavi, Benin, West Africa.
Awadji Jospin Morand Laboratory of Study and Research in Applied Chemistry, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, BP 2009 Abomey-Calavi, Benin, West Africa.
Botezatu Dediu Andreea Laboratory of Organic Chemistry and Biochemistry of the Faculty of Sciences and the Environment of the Dunarea de Jos University of Galati, Domneasca Street 47, 800008 - Galati, Romania.
Olaye Théophile Laboratory of Study and Research in Applied Chemistry, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, BP 2009 Abomey-Calavi, Benin, West Africa.
Agbangnan Dossa Cokou Pascal Laboratory of Study and Research in Applied Chemistry, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, BP 2009 Abomey-Calavi, Benin, West Africa.
Alitonou Guy Alain Laboratory of Study and Research in Applied Chemistry, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, BP 2009 Abomey-Calavi, Benin, West Africa.
Avlessi Félicien Laboratory of Study and Research in Applied Chemistry, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, BP 2009 Abomey-Calavi, Benin, West Africa.
Dinica Rodica Mihaela Laboratory of Organic Chemistry and Biochemistry of the Faculty of Sciences and the Environment of the Dunarea de Jos University of Galati, Domneasca Street 47, 800008 - Galati, Romania.
Sohounhloue Codjo Koko Dominique Laboratory of Study and Research in Applied Chemistry, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, BP 2009 Abomey-Calavi, Benin, West Africa.

DOI:

https://doi.org/10.30574/gscbps.2021.15.3.0148

Keywords:

Skin, Chassalia kolly, secondary metabolies, antioxidant, anti-inflammatory

Abstract

In the last decades, it has been intensively studied natural alternatives to synthetic products. Chassalia kolly is one of the medicinal plants used traditionally for treatment of skin infections. This study aimed at identifying the diversity secondary metabolites, to investigate the antioxidant and anti-inflammatory activities of ethanolic extract of Chassalia kolly leaves. Total phenols were determined by Folin Ciocalteu method. The aluminum trichloride method has been used to quantify total flavonoids, while the determination of condensed tannins was carried out by the hydrochloric vanillin method. The antioxidant capacity was evaluated by 2, 2-diphenyl-l-picrylhydrazyl (DPPH), 2, 2'-Azino-bis- (3-ethylbenzothiazolin-6-sulfonic acid) (ABTS) and phosphomolybdenum methods. In vitro anti-inflammatory activity of ethanolic extract of Chassalia kolly leaves was evaluated by method membrane stabilization. In this results, Chassalia kolly leaves contain flavonoid, mucilages, anthocyans, sterols and terpenes, saponosides, alkaloids and reducing compounds. The contents of total phenols, total flavonoids and total tannins are respectively (20.41±2.11) µgGAE/mgDM; (30.29±2.18) µgQE/mgDM and (1.211±0.122)µgCE/mgDM. The results obtained during this work allowed us to assert that all extracts of the studied plant have very good antioxidant properties (IC₅₀=0.05µg/µL). The ethanolic extract of the leaves of Chassalia kolly showed an anti-inflammatory activity more interesting than aspirin which is the reference compound used in this study.

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References

Akinyemi KO, Oladapo O, Okwara COC, Kehinde AF. Screening of crude extracts of six medicinal plants used in South-West Nigerian unorthodox medicine for anti-methicillin resistant Staphylococcus aureus activity,” BMC Complementary and Alternative Medicine. 2005; 5(1): 1472-1478.

Haejoong K, Soo-Yeon P, and Gihyun L. Potential Therapeutic Applications of Bee Venom on Skin Disease and Its Mechanisms: A Literature Review. Toxins. 2019; 11(7): 374.

Henshaw E, Ibekwe P, Adeyemi A, Ameh S, Ogedegbe E, Archibong J, Olasode O. Dermatologic Practice Review of Common Skin Diseases in Nigeria. International Journal of Health Sciences & Research. 2018; 8(1): 235-248.

Onayemi O, Isezuo SA, Njoku CH. Prevalence of different skin conditions in an outpatients setting in north-western Nigeria. Int J Dermatol. 2005; 44: 7-11.

Wenk C, Itin PH. Epidemiology of pediatric dermatology and allergology in the region of Aargau, Switzerland. Pediatr Dermatol. 2003; 20: 482–7.

WHO. Discussion papers in Child Health. Epidemiology and management of common skin diseases in children in developing countries. WHO/FCH/CAH/05.12. 2005.

Marrone R, Vignally P, Rosso A, Didero D, Pizzini E, Dassoni F, Chiara PM, Maiani E, Franco G, Calcaterra R, Morrone A, Mirisola C. Epidemiology of skin disorders in Ethiopian children and adolescents: an analysis of records from the Italian Dermatological Centre, Mekelle, Tigray, Ethiopia, 2005 to 2009, Pediatr Dermatol. 2012; 29(4): 442-7.

Kiprono S, Baraka CB, Makwaya C, Naafs B, Masenga J. Quality of life of patients with vitiligo attending the Regional Dermatology Training Center in Northern Tanzania. Int J Dermatol. 2013; 52: 191-4.

Burkill HM. The Useful Plants of West Tropical Africa Royal Botanic Garden Kew. 2000; 4: 235-238.

Gustaffson KR, Walton LK, Sowder RCII, Johnson DG, Pannell LK, Cardellina JHII, MR Boyd. J. Nat. Prod. 2000; 63: 176-178.

Onocha PA, Ajaiyeoba EO, Ali MS. In vitro Antileishmaniasis, Phyto and Cytotoxicity of Pycnanthus angolensis Methanolic Extracts. Research Journal of Medical Sciences. 2008; 2(4): 178-181.

Lupoae P, Cristea V, Borda D, Lupoae M, Gurau G, Dinica RM. Phytochemical Screening: Antioxidant and Antibacterial Properties of Potamogeton Species in Order to Obtain Valuable Feed Additives. Journal of Oleo Science. 2015; 1-13.

Oyedapo OO, Akinpelu BA, Akinwunmi KF, Adeyinka MO, Sipeolu FO. Red blood cell membrane stabilizing potentials of extracts of Lantana camara and its fractions. International Journal of Plant Physiology and Biochemistry. 2010; 2(4): 46-51.

Palayullaparambil AKT, Palayullaparambil AKT, Juliet S, Renganathan K, Raju R, Athalathil S, Ravindran R, Chandrashekar L, Nair SN, Ghosh S. Pharmaco-Chemical characterization and Acaricidal Activity of Ethanolic Extract of Chassalia Curviflora (Wall ex Kurz.) Thwaites. Pharmacognosy Journal. 2016; 8(3): 215-219.

Dohou N, Yamni K, Tahrouch S, Idrissi Hassani LM, Badoc A, Gmira N. Screening phytochimique d’une endémique ibéro-marocaine, Thymelaea lythroides. Bulletin de la société pharmaceutique de Bordeaux. 2003; 142: 61-78.

Agbangnan DCP, Tachon CB, Chrostowka A, Fouquet E, Sohounhloue DCK. Phytochemical study of a tinctorial plant of benin traditional pharmacopoeia: The red sorghum (sorghum caudatum) of Benin, Scientific Study & Research. 2012; 13(2): 121-135.

Koudoro YA, Daye ER, Dassou HG, Atindehou M, Agbangnan DCP, Alitonou GA, Avlessi F, Dinica RM, Sohounhloué CKD. Phytochemical screening, antioxidant capacity, antibacterial and anti-inflammatory activities of ethanolic extract of Cordia senegalensis leaves, a plant used in Benin to treat skin diseases. Chemistry Research Journal. 2021; 6(2): 137-146.

Singleton VL, Orthofer R, Lamuela-Raventos RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent. Methods Enzymol. 1999; 299: 152-178.

Mbacke DIS, FalL AD, Diatta-Badji K, Sarr A, Madieye S, Moussa S, Mbaye A, Diatta W, Bassene E. Evaluation de l’activité antioxydante des extraits hydro-ethanoliques des feuilles et écorces de Piliostigma thonningii Schumach. Int. J. Biol. Chem. Sci. 2017; 11(2): 768-776.

Djeridane A, Yous M, Nadjemi B, Boutassouna D, Stocker P, Vidal N. Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chem. 2006; 97: 654-660.

Heimler D, Vignolini P, Giulia M, Francesco VF, Rmani A. Antiradical activity and polyphenol composition of local Brassicaceae edible varieties. Food Chemistry. 2006; 99: 464-469.

Qwele K, Muchenje V, Oyedemi SO, Moyo B, Masika PJ. Effect of dietary mixtures of moringa (Moringa oleifera) leaves, broiler finisher and crushed maize on anti-oxidative potential and physico-chemical characteristics of breast meat from broilers. African Journal of Biotechnology. 2013; 12(3): 290-298.

Bougatef A, Hajji M, Balti R, Lassoued I, Triki-Ellouz Y, Nasri M. Antioxidant and free radical-scavenging activities of smooth hound (Mustelusmustelus) muscle protein hydrolysates obtained by gastrointestinal proteases. Food chem. 2009; 114: 1198-1205.

Sadique J. Al-Rqobahs WA, Bughaith EI-Gindi Ar. The bioactivity of certain medicinal plants on the stabilization of RBS membrane system. Fitoterapia. 1989; 60: 525-532.

Shinde UA, Phadke AS, Nari AM, Mungantiwar AA, Dikshit VJ, Saraf MN. Membrane stabilization activity a possible mechanism of action for the anti-inflammatory activity of Cedrus deodara wood oil. Fitoterapia. 1999; 70: 251-257.

Oyedepo OO, Femurewas AJ. Anti-protease and membrane stabilizing activities of extracts of Fagra zanthoxiloides, Olax subscorpioides and Tetrapleura tetraptera. In. J. Pharm. 1995; 33: 65-69.

Onocha PA, Shaiq MA. Insecticidal, antimicrobial, phyto- and cytotoxicity of Chassalia kolly plant extract. Scholars Research Library. 2010; 2(4): 151-156.

Daddona PE, wright JL, Hutchinson CR. Alkaloid catabolism and mobilization in Catharanthusroseus. Phytochem. 1976; 15: 941-945.

Manolaraki F. Propriétés anthelmintiques du sainfoin (Onobrychisviciifoliae). Analyse des facteurs de variations et du rôle des composés phénoliques impliqués. Thèse de Doctorat de l’université de Toulouse III, Toulouse. 2011.

Bylka W, Matlawska I, Pilewski NA. Natural flavonoids as antimicrobial agents. JANA 7, 24 Ð 31; [31] Iserin Paul (2001). Encclopédie des plantes médicinaes. 2004; 335.

Amelia A, Gujar KN, Kotwal VB, Sonali P. Isolation and evaluation of fenugreek seed as a granulating agent. Ind J Pharm Sci. 2007; 69(5): 676-9.

Ghule BV, Darwhekar GD, Jain DK, Yeole PG. Evaluation of binding properties of Eulophia campestris wall mucilage. Ind J Pharm Sci. 2006; 68(5): 566-9.