Green synthesis of non-cytotoxic silver nanoparticles using Solanum nigrum leaves extract with antibacterial properties

Anushaa  A; Pushpa  Agrawal; Vijaya Kumar  G; Thippareddy  KS

doi:10.30574/gscbps.2021.15.3.0170

Authors

Anushaa A Department of Biotechnology, R.V. College of Engineering, Karnataka, Bangalore-560059, India.
Pushpa Agrawal Department of Biotechnology, R.V. College of Engineering, Karnataka, Bangalore-560059, India.
Vijaya Kumar G Department of Biotechnology, R.V. College of Engineering, Karnataka, Bangalore-560059, India.
Thippareddy KS Department of Biotechnology, R.V. College of Engineering, Karnataka, Bangalore-560059, India.

DOI:

https://doi.org/10.30574/gscbps.2021.15.3.0170

Keywords:

Silver nanoparticle, Solanum nigrum, MTT assay, Mouse fibroblast cell lines

Abstract

Green synthesizes of silver nanoparticles is a promising method in material science and nanotechnology today. Silver nanoparticles (AgNP) were synthesized using a high-efficiency, cost-effective green and simple method at room temperature using Solanum nigrum leaves extract. The biosynthesized AgNP was validated and classified using spectroscopy profiles from ultraviolet-visible spectroscopy, scanning electron microscope, Fourier-transform infrared spectroscopy and x-ray diffraction. FTIR spectra indicated the presence of biological molecules in AgNP synthesis and UV-visible spectra revealed a surface resonance peak of 420 nm corresponding to AgNP formation. The antimicrobial potential of these synthesized nanoparticles is evaluated against the bacteria Escherichia coli (MCC 2079) and Staphylococcus aureus (MCC 2408), both of which are important foodborne pathogens. Ciprofloxacin and Clotrimazole were used as positive controls antibiotic. The nanoparticles were shown to have strong antibacterial efficacy against the strains examined. Their antibacterial function allows them to be included in antimicrobial formulations. By using the MTT assay, the biocompatibility of Solanum nigrum silver nanoparticles were found non-toxic to mouse fibroblast cell lines (L929 is one of the first in continuous culture to be created. The L strain was generated from a male C3H mouse's usual subcutaneous areolar and adipose tissue.) at lower concentrations.

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