The potential use of some spices as immunity booster

Gamal AH  Zaharan; Shams SR  Omima; Hanan A.  Hussien

doi:10.30574/gscbps.2021.16.1.0201

Authors

Gamal AH Zaharan Department of Bread and Pastry Research, Food Technology Research Institute, Agricultural Research Centre, Cairo, Egypt.
Shams SR Omima Department of Bread and Pastry Research, Food Technology Research Institute, Agricultural Research Centre, Cairo, Egypt.
Hanan A. Hussien Department of Bread and Pastry Research, Food Technology Research Institute, Agricultural Research Centre, Cairo, Egypt.

DOI:

https://doi.org/10.30574/gscbps.2021.16.1.0201

Keywords:

Immunity, Cinnamon, Turmeric, Cumin, Black cumin

Abstract

Spices are normally used for flavor to make food aromatic, hot, savory and sweet. Spices not only add aroma to the food but also give a lot of health benefits and nutritional values. Some of the spices used in Egypt are cinnamon, cumin, turmeric, black cumin etc. Many people do not know the importance of spices and its effect on the immune system. The main aim is to high light the role of commonly used spices on immunity. In this review we list some bakery products that use some spices and can be used to improve the immunity of humans.

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References

Devarajan, A. and Mohanmarugaraja, M. K. (2017). A Comprehensive Review on: A South Indian Traditional Functional Food. Pharmacognosy reviews, 11(22), 73–82.

Polovka M, Suhaj M. The Effect of Irradiation and Heat Treatment on Composition and Antioxidant Properties of Culinary Herbs and Spices — A Review’, Food Reviews International. 2010; 26(2): 138– 161.

Nevas M, Korhonen AR, Lindström M, Turkki P, Korkeala H. Antibacterial efficiency of Finnish spice essential oils against pathogenic and spoilage bacteria. Journal of food protection. 2004; 67(1): 199-202.

Centers for Diseases Control and Prevention website. Social distancing: keep your distance to slow the spread. https://www.cdc.gov/coronavirus/2019-ncov/ prevent-getting-sick/social-distancing.html. Published May 10, 2020.

Brooks SK, Webster RK, Smith LE, Woodland L, Wessely S, Greenberg N, Rubin GJ. The psychological impact of quarantine and how to reduce it: rapid review of the evidence. The lancet. 2020; 395(10227): 912-920.

Hawryluck L, Gold WL, Robinson S, Pogorski S, Galea S, Styra R. SARS control and psychological effects of quarantine, Toronto, Canada. Emerging infectious diseases. 2004; 10(7): 1206.

Muscogiuri G, Barrea L, Savastano S, Colao A. Nutritional recommendations for CoVID-19 quarantine. Eur. J. Clin. Nutr. 2020; 74: 850–851.

de Faria Coelho-Ravagnani C, Corgosinho FC, Sanches FLFZ, Prado CMM, Laviano A, Mota JF. Dietary recommendations during the COVID-19 pandemic. Nutrition Reviews. 2021; 79(4): 382-393.

Laviano A, Koverech A, Zanetti M. Nutrition support in the time of SARS-CoV-2 (COVID-19). Nutrition (Burbank, Los Angeles County, Calif.). 2020; 74: 110834.

Caccialanza R, Laviano A, Lobascio F, Montagna E, Bruno R Ludovisi S, Corsico AG, Di Sabatino A, Belliato M, Calvi M, Iacona I. Early nutritional supplementation in non-critically ill patients hospitalized for the 2019 novel coronavirus disease (COVID-19): Rationale and feasibility of a shared pragmatic protocol. Nutrition. 2020; 74: 110835.

Morais AH, Passos TS, Maciel BL, da Silva-Maia JK. Can probiotics and diet promote beneficial immune modulation and purine control in coronavirus infection?. Nutrients. 2020; 12(6): 1737-1749.

Childs CE, Calder PC, Miles EA. Diet and immune function. Nutrients. 2019; 11: 1933.

Monteiro CA, Cannon G, Moubarac JC, Levy RB, Louzada MLC, Jaime PC. The UN Decade of Nutrition, the NOVA food classification and the trouble with ultra-processing. Public Health Nutr. 2018; 21: 5–17.

Maret W. Cellular zinc and redox states converge in the metallothionein/thionein pair. J Nutr. 2003; 133(5 Suppl 1):1460S–2S.

Mocchegiani E, Malavolta M. Role of zinc and selenium in oxidative stress and immunosenescence: Implications for healthy aging and longevity. Handbook of immunosenescence: Basic understanding and clinical implications. 2019; 2539–73.

Zhang L, Liu Y. Potential interventions for novel coronavirus in China: A systematic review. Journal of Medical Virology. 2020; 92(5): 479-90.

Guastalegname M, Vallone A. Could chloroquine/hydroxychloroquine be harmful in coronavirus disease 2019 (COVID-19) treatment?. Clinical Infectious Diseases. 2020; 71(15): 888-889.

Lassi ZS, Moin A, Bhutta ZA. Zinc supplementation for the prevention of pneumonia in children aged 2 months to 59 months. Cochrane Database of Systematic Reviews. 2016; (12).

Huang Z, Rose AH, Hoffmann PR. The role of selenium in inflammation and immunity: From molecular mechanisms to therapeutic opportunities. Antioxidants & Redox Signaling. 2012; 16(7): 705-43.

Arthur JR, McKenzie RC, Beckett GJ. Selenium in the immune system. The Journal of Nutrition. 2003; 133(5): 1457S-9S.

Kiremidjian-Schumacher L, Roy M, Wishe HI, Cohen MW, Stotzky G. Supplementation with selenium augments the functions of natural killer and lymphokine-activated killer cells. Biological Trace Element Research. 1996; 52(3): 227- 39.

Coquette A, Vray B, Vanderpas J. Role of vitamin E in the protection of the resident macrophage membrane against oxidative damage. Archives Internationales de Physiologie et de Biochimie. 1986; 94(5): S29-34.

Chavance M, Herbeth B, Fournier C, Janot C, Vernhes G. Vitamin status, immunity and infections in an elderly population. European Journal of Clinical Nutrition. 1989; 43(12): 827-35.

Meydani SN, Leka LS, Fine BC, Dallal GE, Keusch GT, Singh MF, et al. Vitamin E and respiratory tract infections in elderly nursing home residents: A randomized controlled trial. JAMA. 2004; 292(7):828-36.

Wooltorton E. Too much of a good thing? Toxic effects of vitamin and mineral supplements. CMAJ. 2003; 169(1): 47-8.

Villamor E, Fawzi WW. Effects of vitamin A supplementation on immune responses and correlation with clinical outcomes. Clinical Microbiology Reviews. 2005; 18(3): 446-64.

Ross AC. Vitamin A and retinoic acid in T cell–related immunity. The American Journal of Clinical Nutrition. 2012; 96(5): 1166S-72S.

Brown CC, Noelle RJ. Seeing through the dark: new insights into the immune regulatory functions of vitamin A. European Journal of Immunology. 2015; 45(5):1287- 95.

Erkelens MN, Mebius RE. Retinoic acid and immune homeostasis: A balancing act. Trends in Immunology. 2017; 38(3): 168-80.

Oliveira LdM, Teixeira FME, Sato MN. Impact of retinoic acid on immune cells and inflammatory diseases. Mediators of Inflammation. 2018.

Yoshii K, Hosomi K, Sawane K, Kunisawa J. Metabolism of dietary and microbial vitamin B family in the regulation of host immunity. Frontiers in Nutrition. 2019; 6: 48.

Tamura J, Kubota K, Murakami H, Sawamura M, Matsushima T, Tamura T, Saitoh T, Kurabayshi H, Naruse T. Immunomodulation by vitamin B12: augmentation of CD8+ T lymphocytes and natural killer (NK) cell activity in vitamin B12‐deficient patients by methyl‐B12 treatment. Clinical & Experimental Immunology. 1999; 116(1): 28-32.

Sharma M, Gupta A, Prasad R. A Review on Herbs, Spices and Functional Food Used in Diseases. International Journal of Research & Review. 2017; 4(1): 103-108.

Frydman-Marom A, Levin A, Farfara D, Benromano T, Scherzer-Attali R, Peled S, Vassar R, Segal D, Gazit E, Frenkel D, Ovadia M. Orally administrated cinnamon extract reduces β-amyloid oligomerization and corrects cognitive impairment in Alzheimer's disease animal models. PloS one. 2011; 6(1): p.e16564.

Food and Drug Administration (FDA). Code of federal regulations (CFR). Title 21: Food and drugs. Chapter I e food and drug administration, department of health and human services, subchapter B-e food for human consumption (continued). Part 182 e Substances Generally Recognized as Safe (GRAS), Subpart A e General Provisions, Subpart 182.20 e Essential oils, oleoresins, and natural extractives. Office of the Federal Register, Washington, (Revised April, 2016). 2015.

Sana S, Arshad MU, Saeed F, Ahmad R, Imran A, Tufail T. Nutritional characterization of cinnamon and turmeric with special reference to their antioxidant profile. International Journal of Biosciences. 2019; 15(4): 178-187.

Balasubramanian S, Roselin P, Singh KK, Zachariah J, Saxena SN. Postharvest processing and benefits of black pepper, coriander, cinnamon, fenugreek, and turmeric spices. Critical reviews in food science and nutrition. 2016; 56(10): 1585-1607.

Ribeiro-Santos R, Andrade M, Madella D, Martinazzo AP, Moura LDAG, de Melo NR, Sanches-Silva A. Revisiting an ancient spice with medicinal purposes: Cinnamon. Trends in Food Science & Technology. 2017; 62: 154-169.

Thomas J, Kuruvilla KM. Cinnamon. In In handbook of herbs and spices. Woodhead Publishing Limited. 2012; 182-196.

Gruenwald J, Freder J, Armbruester N. Cinnamon and health. Critical Reviews in Food Science and Nutrition. 2010; 50(9): 822-834.

Ho SC, Chang KS, Chang PW. Inhibition of neuroinflammation by cinnamon and its main components. Food Chemistry. 2013; 138(4): 2275-2282.

Brahmachari S, Jana A, Pahan K. Sodium benzoate, a metabolite of Cinnamon and a food additive, reduces microglial and astroglial inflammatory responses. The Journal of Immunology. 2009; 183(9): 5917-5927.

Hajimonfarednejad M, Ostovar M, Raee MJ, Hashempur MH, Mayer JG, Heydari M. Cinnamon: A systematic review of adverse events. Clin Nutr. 2019; 38: 594‑602.

Gunawardena D, Govindaraghavan S, Münch G. Anti-inflammatory properties of Cinnamon polyphenols and their monomeric precursors. In In polyphenols in human health and disease. 2014; 1: 409-425.

Lv J, Huang H, Yu L, Whent M, Niu Y, Shi H, Wang TT, Luthria D, Charles D, Yu LL. Phenolic composition and nutraceutical properties of organic and conventional cinnamon and peppermint. Food Chemistry. 2012; 132(3): 1442-1450.

Qin B, Nagasaki M, Ren M, Bajotto G, Oshida Y, Sato Y. Cinnamon extract (traditional herb) potentiates in vivo insulin-regulated glucose utilization via enhancing insulin signaling in rats. Diabetes research and clinical practice. 2003; 62(3): 139-148.

Muthuswamy S, Rupasinghe HPV, Stratton GW. Antimicrobial effect of cinnamon bark extract on Escherichia Coli O157:H7, listeria Innocua and fresh-cut apple slices. Journal of Food Safety. 2008; 534–549.

Kuspradini H, Putri AS, Sukaton E, Mitsunaga T. Bioactivity of essential oils from leaves of dryobalanops Lanceolata, Cinnamomum burmannii, Cananga odorata, and scorodocarpus borneensis. Agriculture and Agricultural Science Procedia. 2016; 9: 411-418.

Przygodzka M, Zieli_nska D, Ciesarov_a Z, Kukurov_a K, Zieli_nski H. Comparison of methods for evaluation of the antioxidant capacity and phenolic compounds in common spices. LWT - Food Science and Technology. 2014; 58(2): 321-326.

Sharma V, Rao LJM. An overview on chemical composition, bioactivity and processing of leaves of Cinnamomum tamala. Critical Reviews in Food Science and Nutrition. 2014; 54(4): 433-448.

Anderson RA, Zhan Z, Luo R, Guo X, Guo Q, Zhou J, Kong J, Davis PA, Stoecker BJ. Cinnamon extract lowers glucose, insulin and cholesterol in people with elevated serum glucose. Journal of traditional and complementary medicine. 2016; 6(4): 332-336.

Modi KK, Roy A, Brahmachari S, Rangasamy SB, Pahan K. Cinnamon and its metabolite sodium benzoate attenuate the activation of p21rac and protect memory and learning in an animal model of Alzheimer's disease. PLoS One. 2015; 10(6): e0130398.

Cheng DM, Kuhn P, Poulev A, Rojo LE, Lila MA, Raskin I. In vivo and in vitro antidiabetic effects of aqueous cinnamon extract and cinnamon polyphenol-enhanced food matrix. Food Chemistry. 2012; 135(4): 2994-3002.

Kim EC, Kim HJ, Kim TJ. Water extract of Cinnamomum cassia suppresses angiogenesis through inhibition of VEGF receptor 2 phosphorylation. Bioscience, Biotechnology, and Biochemistry. 2015; 79(4): 617-624.

Kim JE, Son JE, Jeong H, Kim DJ, Seo SG, Lee E, Lim TG, Kim JR, Kimbung YR, Chen H, Bode AM. A novel cinnamon-related natural product with Pim-1 inhibitory activity inhibits leukemia and skin cancer. Cancer research. 2015; 75(13): 2716-2728.

Ramadan MF, Moersel JT. Oxidative stability of black cumin (Nigella sativa L.), coriander (Coriandrum sativum L.) and niger (Guizotia abyssinica Cass.) upon stripping. European Journal of Lipid Science and Technology. 2004; 106: 35–43.

Duke JA. Handbook of Phytochemical Constituents of GRAS Herbs and other Economic Plants. Pp. 35–69. Florida, FL: CRC Press, Inc. 1992.

Al-Gaby AM. Amino acid composition and biological effects of supplementing broad bean and corn proteins with Nigella sativa (Black cumin) cake protein. Nahrung. 1998; 42: 290–294.

Takruri HRH, Dameh MAF. Study of the nutritional value of black cumin seeds (Nigella sativa). Journal of the Science of Food and Agriculture. 1998; 76: 404–410.

Amin B, Hosseinzadeh H. Black Cumin (Nigella sativa) and Its Active Constituent, Thymoquinone: An Overview on the Analgesic and Anti-inflammatory Effects. Planta Med. 2016; 82: 8–16.

Sultan MT, Butt MS, Anjum FM, Jamil A, Akhtar S, Nasir M. Nutritional profile of indigenous cultivar of black cumin seeds and antioxidant potential of its fixed and essential oil. Pak J Bot. 2009; 41: 1321–1330.

Hadad GM, Salam RA, Soliman RM, Mesbah MK. High-performance liquid chromatography quantification of principal antioxidants in black seed (Nigella sativa L.) phytopharmaceuticals. J AOAC Int. 2012; 95: 1043–1047.

Latiff LA, Parhizkar S, Dollah MA, Hassan ST. Alternative supplement for enhancement of reproductive health and metabolic profile among perimenopausal women: a novel role of Nigella sativa. Iran J Basic Med Sci. 2014; 17: 980–985.

Burits M, Bucar F. Antioxidant activity of Nigella sativa essential oil. Phytother Res. 2000; 14: 323–328.

Singh S, Das SS, Singh G, Schuff C, de Lampasona MP, Catalan CA. Composition, in vitro antioxidant and antimicrobial activities of essential oil and oleoresins obtained from black cumin seeds (Nigella sativa L.). Biomed Res Int. 2014.

Al-Saleh IA, Billedo G, El-Doush II. Levels of selenium, dl-α-tocopherol, dl-γ-tocopherol, all-trans-retinol, thymoquinone and thymol in different brands of Nigella sativa seeds. J Food Compost Anal. 19: 167–175.

Muhammad TS. Characterization of black cumin seed oil and exploring its role as a functional food. Faisalabad: University of Agriculture. 2009.

El-Tahir Kamal EH, Bakeet DM. The Black seed Nigella sativa Linnaeus –a mine for multi cures: a plea for urgent clinical evaluation of its volatile oil. J T U Med Sci. 2006; 1: 1–19.

Awad E, Austin D, Lyndon AR. Effect of black cumin seed oil (Nigella sativa) and nettle extract (Quercetin) on enhancement of immunity in rainbow trout, Oncorhynchus mykiss (Walbaum). Aquaculture. 2013; 388: 193–197.

Ibrahim RM, Hamdan NS, Mahmud R, Imam MU, Saini SM, Rashid SN, Abd Ghafar SA, Latiff LA, Ismail M. A randomised controlled trial on hypolipidemic effects of Nigella Sativa seeds powder in menopausal women. J Transl Med. 2014; 12(1): 1-7.

Hosseini M, Mohammadpour T, Karami R, Rajaei Z, Sadeghnia HR, Soukhtanloo M. Effects of the hydro-alcoholic extract of Nigella sativa on scopolamine-induced spatial memory impairment in rats and its possible mechanism. Chin J Integr Med. 2015; 21: 438–444.

Bamosa AO. A review on the hypoglycemic effect of Nigella sativa and thymoquinone. Saudi Journal of Medicine and Medical Sciences. 2015; 3(1): 2.

Entok E, Ustuner MC, Ozbayer C, Tekin N, Akyuz F, Yangi B, Gunes HV. Anti-inflammatuar and antioxidative effects of Nigella sativa L.: 18FDG-PET imaging of inflammation. Molecular Biology Reports. 2014; 41(5): 2827-2834.

Al-Ghamdi MS. The anti-inflammatory, analgesic and antipyretic activity of Nigella sativa. J Ethnopharmacol. 2001; 76: 45–48.

Kamal A, Arif JM, Ahmad IZ. Potential of Nigella sativa L. seed during different phases of germination on inhibition of bacterial growth. J Biotech Pharm Res. 2010; 1: 9-13.

Leong XF, Rais Mustafa M, Jaarin K. Nigella sativa and its protective role in oxidative stress and hypertension. Evidence-Based Complementary and Alternative Medicine. 2013; 1-9.

Shafiq H, Ahmad A, Masud T, Kaleem M. Cardio-protective and anti-cancer therapeutic potential of Nigella sativa. Iranian Journal of Basic Medical Sciences. 2014; 17(12): 967.

Aggarwal BB, Sundaram C, Malani N, Ichikawa H. Curcumin: the Indian solid gold. The molecular targets and therapeutic uses of curcumin in health and disease Springer, Boston, MA. 2007; 1- 75.

Akter J, Hossain MA, Takara K, Islam MZ, Hou DX. Antioxidant activity of different species and varieties of turmeric (Curcuma spp): Isolation of active compounds. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology. 2019; 215: 9-17.

Prathapan A, Lukhmari M, Arumughan C, Sundaresan A, Raghu KG. Effect of heat treatment on curcuminoid, colour value and total polyphenols of fresh turmeric rhizome. Int J Food Science Technology. 2009; 44: 1438-1444.

Olatunde-Farombi E, S Sangeefc, TFN Hye-Kyung, K Sung-Hoon, S Young-Joon. Curcumin attenuates dimethyl-nitroseamine-induced liver injury in rats through Nrf2-mediated induction of heme oxygenase-1. Food and Chemical Toxicology. 2008; 46(4): 1279-1287.

Singh G, IPS Kapoor, P Singh, CSD Helnani, MPD Lampasona, CAN Catalan. Comparative study of chemical composition and antioxidant activity of fresh and dry rhizomes of turmeric (Curcuma longa Linn.). Food Chemical Toxicology. 2010; 48(4): 1026– 1031.

Lim HS, SH Park, K Ghafoor, SY Hwang, JY Park. Quality and antioxidant properties of bread containing turmeric (Curcuma longa L.) cultivated in South Korea. Food Chemistry. 2011; 124(4): 1577–1582.

El-Gohery SS. Quality Aspects for High Nutritional Value Pretzel. Current Science International. 2020; 9(4): 583-593.

EL-Bedawy AA, Mansour EH, EL-Beltagy AE, Zahran GA, Badrm W. Producing of gluten free biscuits for celiac patients. Minufiya J. Agric. Res. 2009; 34 (4): 1573-1586.

Mohamed ES, Shams OS, Hussien HA, El-Adly NA. Production of High Nutritional Value Gluten Free Crackers with Sesame and Turmeric Powder. Egypt. J. Food. Sci. 2020; 48(2): 291-302.

Karami M, Alimon AR, Sazili AQ, Goh YM, Ivan M. Effects of dietary antioxidants on the quality, fatty acid profile, and lipid oxidation of longissimus muscle in Kacang goat with aging time. Meat Science. 2011; 88: 102-108.

Negi PS, Jayaprakasha GK, Jagan MRL, Sakariah KK. Antibacterial activity of turmeric oil: a byproduct from curcumin manufacture. Journal of Agricultural and Food Chemistry. 1999; 47(10): 4297-4300.

Menon V, Sudheer A. Antioxidant and anti-inflammatory properties of curcumin. Adv Exp Med Biol. 2007; 595: 105-125.

Hewlings SJ, Kalman DS. Curcumin: a review of its effects on human health. Foods. 2017; 6(10): 92.

Reddy RC, Vatsala PG, Keshamouni VG, Padmanaban G, Rangarajan PN. Curcumin for malaria therapy. Biochem. Biophys. Res. Commun. 2005; 326: 472–474.

Di Mario F, Cavallaro LG, Nouvenne A, Stefani N, Cavestro GM, Iori V, Maino M, Comparato G, Fanigliulo L, Morana E, Pilotto A. A curcumin‐based 1‐week triple therapy for eradication of Helicobacter pylori infection: something to learn from failure?. Helicobacter. 2007; 12(3): 238-243.

Chattopadhyay I, Biswas K, Bandyopadhyay U, Banerjee RK. Turmeric and curcumin: biological actions and medicinal applications. Curr Sci India. 2004; 87: 44-53.

Sahoo JP, Mohapatra U, Mishra AP, Chandra K. Turmeric (Haldi)-A strapping strategy for enhancing the immune system to reduce the effect of SARS-CoV-2. Food and Scientific Reports. 2020; 1(8): 10.

Singh RP, Gangadharappa HV, Mruthunjaya K. Cuminum cyminum–A popular spice: An updated review. Pharmacognosy Journal. 2017; 9(3).

Bettaieb I, Bourgou S, Sriti J, Msaada K, Limam F, Marzouk B. Essential oils and fatty acids composition of Tunisian and Indian cumin (Cuminum cyminum L.) seeds: a comparative study. Journal of the Science of Food and Agriculture. 2011; 91: 2100–2107.

Lewis YS. Spices and herbs for the Food Industry, England: Food Trade press. 1984; 121–122.

Merah O, Sayed-Ahmad B, Talou T, Saad Z, Cerny M, Grivot S, Evon P, Hijazi A. Biochemical composition of cumin seeds, and biorefining study. Biomolecules. 2020; 10(7): 1054.

Moawad SA, El-Ghorab AH, Hassan M, Nour-Eldin H, El-Gharabli MM. Chemical and microbiological characterization of Egyptian cultivars for some spices and herbs commonly exported abroad. Food and Nutrition Sciences. 2015; 6(07): 643.

Milan KM, Dholakia H, Tiku PK, Vishveshwaraiah P. Enhancement of digestive enzymatic activity by cumin (Cuminum cyminum L.) and role of spent cumin as a bionutrient. Food chemistry. 2008; 110(3): 678-683.

Anon A. Analytical methods of committee. Application of gas-liquid chromatography to analysis of essential oils Part XVI: Monography for five essential oils. Analyst. 1993; 118: 1089–1098.

Shaath NA, Azzo NR. Essential oil of Egypt. In: G. Charalambous (Ed.): Food Flavor Ingredients and Composition. Charalambous, G., Ed., Elsevier, Amsterdam. 1993; 591–603.

Krishnakantha TP, Lokesh BR. Scanvenging of superoxide anions by spice principles. Indian J Biochem Biophys. 1993; 30: 133–134.

Kivanc M, Akgul A, Dogan A. Inhibitory and stimulatory effects of cumin, oregano and their essential oils on growth and acid production of Lactobacillus planatarum and Leuconstoc mesenteroides. Int. J. Food Microbiol. 1991; 13: 81–85.

Iacobellis NS, Cantore PL, Capasso F, Senatore F. Antibacterial activity of Cuminun cyminum L. and Carum carvi L essential oils. J. Agric. Food Chem. 2005; 53: 57–61.

Doweidar, Mona MM, Amer Thanaa AM. Production and evaluation of highly protein bakery products using legumes. Annals of Agric. Sc., Moshtohor. 2005; 43(4): 1779-1795.

El-Gohery El-Dian S. Mohamed, Ashraf M Sharaf, Houssin K Ashour, Doweidar Mona MM and Mohamed SI Saleh. Sensory and biological evaluation of biscuits supplemented with black cumin (Nigella sativa L.) and its extracts. Egpt. J. of Appl. Sci. 2008; 23(11B): 579-599.

Abdelazim AS. Technochemical and biological studies on some spices and their volatile oils used in bakery products. Thesis (M.Sc.) Faculty of agric., Cairo University. 2007.

Sharoba AMRM, El-Saadany El-Gharabli MM, Abd El-Rheheem EM, Abd El-Salam A. Chemical, sensory and biological evaluation of batun salat fortified with some herbs and bee pollen grains as a nutritive treatment for iron deficiency anemia. Egypt. J. Agric Res. 2013; 91(1): 13-42.