Bacteriological and heavy metal assessment of a tropical crude oil- polluted soil, water and sediment
DOI:
https://doi.org/10.30574/gscbps.2021.16.2.0237Keywords:
Bacteria, Heavy metals, Hydrocarbon, Niger Delta, PollutionAbstract
Crude oil-polluted environment serves as a reservoir of microbial species, especially bacteria. Determining these bacterial genera is of great benefit to environmental assessment and recovery. This study evaluated the bacteria and selected heavy metals present in crude oil-polluted sites in K-dere community, Ogoniland. The study was carried out at the Department of Microbiology, University of Port Harcourt, Rivers State, Nigeria. Crude oil-polluted soil, water, and sediment samples were collected from K-dere community, Ogoniland. The pH and heavy metals composition of each sample was determined using pH meter and spectrophotometer, respectively, while isolation and biochemical characterization were done to evaluate the heterotrophic and hydrocarbon utilizing bacteria present in each sample. Results revealed that iron (Fe) had the highest concentration in the soil (898.77±0.022 mg/kg) and sediment (1556.7±0.163 mg/kg) samples, while lead (Pb) had the lowest overall concentration. The concentration of total petroleum hydrocarbon (TPH) (10410.5 mg/kg) in soil sample exceeded the DPR standard limit of 5000 mg/kg for soil. The results of total culturable heterotrophic bacterial counts (TCHBC) and total culturable hydrocarbon utilizing bacterial counts (TCHUBC) showed that soil and sediment samples had the highest TCHBC and TCHUBC values of 2.66 ± 0.03 x 108 CFU/mL and 4.7 ± 0.14 x 107 CFU/mL respectively. Morphological and biochemical characterization of the isolates revealed the presence of Pseudomonas spp, Bacillus spp, Acidiphilium spp, Mycobacterium spp and Leptospirillum spp in the samples with Pseudomonas spp having the highest percentage occurrence. This study has revealed the presence of useful bacterial species in the sampled sites which can be harnessed for an in situ cleaning of crude oil- contaminated site, especially in the tropical region.
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References
Achife CE, Joshua U, Bala J, Oyeleke S. Microbial Population of Soil and H2O Around Petroleum Depot, Suleija, Nigeria and their Hydrocarbon Utilization. International Journal of Life Science and Biotechnology. 2020; 4(1): 90-113.
Chikere CB, Okpokwasili GC, Chikere BO. Bacterial Diversity in a Tropical Crude Oil – polluted Soil Undergoing Bioremediation. African Journal of Biotechnology. 2009; 8(11): 2535-40.
Okafor CP, Udemang NL, Chikere CB, Akaranta O, Ntushelo K. Indigenous Microbial Strains as Bioresource for Remediation of Chronically Polluted Niger Delta Soils. Africa Scientific Research Journal. 2021; 11(1): 2468 –76.
Akpoveta VO, Egharevba F, Medjor WO, Osaro KI, Enyemike ED. Microbial Degradation and its Kinetics on Crude Oil – polluted Soil. Research Journal of Chemical Sciences. 2011; 1(16): 4 -14.
Chikere CB, Mordi IJ, Chikere BO, Selvarajan R, Ashafa TO, Obieze CC. Comparative Metagenomics and Functional Profiling of Crude Oil-contaminated Soil in Bodo West community, Ogoni, with Other Sites of Varying Pollution History. Annals of Microbiology. 2019; 69: 495 – 513.
Bates RA. Electrometric Determination. Ist Edition. John Wiley Sons, Inc. New York. 1954.
Singh RA.Soil Physical Analysis. Kalyana Publishers, New Delhi Ludhiana. 1980; 8.
Ezebuiro V, Otaraku IJ, Oruwari B, Okpokwasili GC. Viability of Hydrocarbon-degrading Bacterial Consortium Immobilized on different carriers. Biotechnology Journal International. 2019; 23(4): 1-9.
APHA.Standard Methods for the Examination of Water and Waste Water. 21stEd. American Public Health Association Inc. /American Water Works Association / Water Environment Federation, Washington DC. 2005.
Holt JG. Bergey’s Manual of Determinative Bacteriology. 9th ed. 1994.
Department of Petroleum Resources. Environmental Guidelines and Standards for the Petroleum Industry (Egaspin) Lagos. 2002.
Radulescu C, Stihi C, Popescu IV, Lucica GT, Chelarescu ED, Stirbescu R. Assessment of Heavy Metals Content of Crude Oil-contaminated Soil. Journal of Science and Arts. 2021; 4(21): 459 – 468.
Uba BO, Chukwura EI, Okoye EL, Onyedikachi U, Irabor MI, Onyekwuluje NV, Ajeh JE, Mmuogbo CS, Nwafor MC, Igboesorom CC, Nwodo CJ, Okafor JC, Nwachukwu CJ. Multiple Degradation and Resistance Capabilities of Marine Bacterial Isolated from Niger Delta, Nigeria on Petroleum Pollutants and Heavy Metals. Journal of Advances in Biology & Biotechnology. 2018; 20(1): 1-17.
Ololade IA. An Assessment of Heavy-metal Contamination in Soils within Auto-mechanic Workshops using Enrichment and Contamination Factors with Geoaccumulation Indexes. Journal of Env. Prot. 2014; 5: 974.
Adoki A. Soil and Groundwater Characteristics of a Legacy Spill Site. Journal of Applied Sciences, Environment and Management. 2012; 16(1): 113 – 123.
Edwin – Wosu NL, Albert E. Total Petroleum Hydrocarbon Content as an Index Assessment of Macrophytic Remediation Process of a Crude Oil-contaminated Soil Environment. Journal of Applied Science Environmental Management. 2010; 14 (1): 39 – 42.
Uba BO, Akunna CM, Okemadu CO, Umeh CJ. Kinetics of Biodegradation of Total Petroleum Hydrocarbon in Diesel Contaminated Soil as Mediated by Organic and Inorganic Nutrients. Animal Research International Journal. 2019;16 (2): 3295 – 3307.
Ezekoye CC, Amakoromo ER, Ibiene AA. Lab-based Bioremediation of Hydrocarbon-polluted Mangrove Swamp Soil in the Niger Delta sing Poultry Wastes. Microbiological Research Journal International. 2017; 19 (2): 1 – 14.
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