Evaluation of the anti-inflammatory activity of a gel based on Afzelia africana (fabaceae) leaves

Authors

  • Pondy Bias Mathilde Audrey Galenic Pharmacy Laboratory ; Université des Montagnes ; BP 208 Bangangté; Cameroon.
  • Djoko Ernest Galenic Pharmacy Laboratory ; Université des Montagnes ; BP 208 Bangangté; Cameroon.
  • Foutse Yimta Wandji Laboratory of Pharmacognosy; Université des Montagnes ; BP 208 Bangangté ; Cameroon.
  • Wona Jean Pierre Ciniques Universitaires des Montagnes ; Bangangté ; Cameroon.
  • Wouessidjewe Denis University of Grenoble Alpes-UFR de Pharmacy, Department of Molecular Pharmacochemistry- UMR CNRS 5063. France.

DOI:

https://doi.org/10.30574/gscbps.2021.16.3.0262

Keywords:

Afzelia africana, Leaf extract, Topical hydrogel, Anti-inflammatory

Abstract

Afzelia africana is a plant used by traditional healers in the treatment of several inflammatory pathologies, in particular edema, rheumatism, lumbago. The effectiveness of its barks in treating inflammation is well established. Ethnobotanical data led researchers to formulate a stable topical hydrogel based on bark extract, the tolerance and effectiveness of which in animals have been found to be satisfactory. As excessive use of the bark may impair the survival of the plant, further leaf studies have been performed and the anti-inflammatory effect has been confirmed in rats. The objective of the present study was to evaluate the anti-inflammatory efficacy of a topical gel based on the hydro-ethanolic extract of these leaves in patients with inflammatory symptoms, compared to a diclofenac gel.

Using hydroxyethyl cellulose and glycerol a stable gel was produced and used for the evaluation of the anti-inflammatory activity of the leaves of the plant. The dry extract obtained from the leaves was rich in flavonoids (57.26 mg quercetin equivalent per gram), phenols, tannins, alkaloids, anthraquinones and steroids. We carried out a randomized pilot study on 52 patients who came for consultation at the « Cliniques Universitaires des Montagnes » and at the Bangangté District Hospital for inflammatory symptoms between June 1 and July 17, 2020; pregnant women were excluded from the study. Topical administration on 15 inflammatory pathologies revealed that the Afzelia africana leaf extract gel exhibits a better onset of action (4.29 ± 1.89 min) than the diclofenac gel (16.96 ± 4, 73 min). The decrease in pain visual analogue scale (VAS): values ​​for Afzelia africana gel was greater than for diclofenac gel, with P < 0.05 on day 2 and P < 0.001 from day 3 to day 7. The difference in First Hour Sedimentation Rate was significant between the two gels (P < 0.05); but this difference was no longer statistically significant after the second hour (P ˃ 0.05). In addition, Afzelia africana leaf extract gel reduced swelling (P = 0.001) and stiffness (P < 0.001) better than diclofenac gel.

These results clarify the effectiveness of Afzelia africana leaf extract gel in the management of inflammatory pathologies and / or symptoms.

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References

Ngene JP, Ngoule CC, Kidik CMP, Ottou PM, Dibong SD, Mpondo EM. Importance in the traditional pharmacopoeia of flavonoid plants sold in the markets of Douala East (Cameroon). J Appl Biosci. 2015; 88 (1): 8194–8210.

Nolé TT, Guedje NM, Nnanga N. Production of improved traditional medicines in Cameroon: case of an oral hypoglycemic agent. Health Sci Dis. 2013; 13 (3).

Babulka P. Medicinal plants for the treatment of rheumatic pathologies: from traditional medicine to modern phytotherapy. Phytotherapy. 2007; 5 (3): 137 45.

Heymonet C. Anti-inflammatory plants used in herbal medicine. Pharmacy thesis. University of Lorraine. 2013.

Fuck YW. Ethnobotanical survey of medicinal plants used in the West Cameroon region: Phytochemical and pharmacological study of Afzelia africana J.E. Smith. Third cycle doctoral thesis. University of Aix Marseille. 2017.

Kengne Kamkui L. Formulation and evaluation of the anti-inflammatory activity in vivo of a topical hydrogel based on extract of the bark of Afzelia africana (Fabaceae) in rats. Doctorate in Pharmacy. “Université des Montagnes”-Bangangté. 2018.

Tebue Kouam RL. Phytochemical study and determination of the anti-inflammatory activity of aqueous and hydro-ethanolic extracts of the leaves of Afzelia africana (Fabaceae). Doctorate in Pharmacy. “Université of the Montagnes”-Bangangté. 2019.

Bekro YA, Mamyrbekova J, Boua B, Bi F, Ehile E. Ethnobotanical study and phytochemical screening of Caesalpinia benthamiana (Baill.) Herend. and Zarucchi (Caesalpiniaceae). Sci Nat. 2007; 4 (2): 217 25.

Kom Kamsu A. Comorbidities and mortality associated with rheumatoid arthritis in patients at the Yaoundé Central Hospital. University of Yaoundé I. 2015.

Nolé TT, Guedje NM, Nnanga N. Production des médicaments traditionnels améliorés au Cameroun : cas d’un hypoglycémiant oral. Health Sci Dis. 2013; 13(3).

Babulka P. Plantes médicinales du traitement des pathologies rhumatismales: de la médecine traditionnelle à la phytothérapie moderne. Phytothérapie. 2007; 5(3): 137 45.

Heymonet C. Les plantes à visée anti-inflammatoire utilisées en phytothérapie. Thèse de pharmacie. Université de Lorraine. 2013.

Foutse YW. Enquête ethnobotanique sur les plantes médicinales utilisées dans la région de l’Ouest Cameroun: Etude phytochimique et pharmacologique de Afzelia africana J.E.Smith. Thèse de doctorat de Troisième cycle. Université d’Aix Marseille. 2017.

Kengne Kamkui L. Formulation et évaluation de l’activité antiinflammatoire in vivo d’un hydrogel topique à base de l’extrait de l’écorce de Afzelia africana (Fabaceae) chez les rats. Doctorat en Pharmacie. Université des Montagnes-Bangangté. 2018.

Tebue Kouam RL. Etude phytochimique et détermination de l’activité anti-inflammatoire des extraits aqueux et hydro-éthanolique des feuilles de Afzelia africana (Fabaceae). Doctorat en Pharmacie. Université des Montagnes-Bangangté. 2019.

Bekro YA, Mamyrbekova J, Boua B, Bi F, Ehile E. Étude ethnobotanique et screening phytochimique de Caesalpinia benthamiana (Baill.) Herend. et Zarucchi (Caesalpiniaceae). Sci Nat. 2007; 4(2): 217 25.

Kom Kamsu A. Comorbidités et mortalité associées à la polyarthrite rhumatoïde chez les patients de l’Hôpital Central de Yaoundé. Université de Yaoundé I. 2015.

Hugh A. Gemmell, DC, Bert HJ, Brad MH. Effect of a topical herbal cream on osteoarthritis of the hand and knee:apilot study. J Manipulative Physiol Ther. 2003; 26: 15.

Castanedo-Cázares JP, Quistián-Hemández J, TorresAlvarez B. Anti-inflammatory effect of a topical corticosteroid: known brand versus generic drug. Gac Méd Méx. 2001; 137(4): 311-4.

Bourreau F, Schneid H, Zeghari N, Bourgeois P. The IPSO study: ibuprofen, paracetamol study in osteoarthritis. A randomised comparative clinical study comparing the efficacy and safety of ibuprofen and paracetamol analgesic treatment of osteoarthritis of the knee or hip. Ann Rheum Dis. 2004; 63: 1028-1034.

Moghadam Nia A, Heidari B, Pourhadi M, Baradaran M. Comparison of the therapeutic effects of effective material of red pepper and Diclofenac gel in treatment of osteoarthritis. JBUMS. 2003; 5(3): 12-17.

Sengupta S, Velpadian T, Kabir SR, Gupta SK. Analgesic efficacy and pharmacokinetics of topical nimesulide gel in healthy human volunteers: double blind comparison with piroxicam, diclofenac and placebo. Eur J Clin Pharmacol. 1998; 54: 541-547.

Williams CA, Hoult J, Harborne JB, Greenham J, Eagles J. A biologically active lipophilic flavonol from Tanacetum parthenium. Phytochemistry. 1995; 38(1): 267-70.

Mali A, Bandawane D, Hivrale MG. Antiinflammatory and analgesic activities of ethyl acetateand petroleum ether fractions of Cassia auriculata Linn. Leaves. Oriental Pharmacy and Experimental Medicine. 2013; 13(3): 191-197.

Shekhawat N, Vijayvergia R. Investigation of AntiInflammatory, Analgesic and Antipyretic Properties of Madhuca indica Gmel. European Journal of Inflammation. 2018; 8(3): 165-171.

Wagner H. Search for new plant constituents with potential anti-phlogistic and anti-allergic activity. Planta Medica. 1989; 55: 235-241.

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Published

2021-09-30

How to Cite

Audrey, P. B. M. ., Ernest, D. ., Wandji, F. Y. ., Pierre, W. J. ., & Denis, W. . (2021). Evaluation of the anti-inflammatory activity of a gel based on Afzelia africana (fabaceae) leaves. GSC Biological and Pharmaceutical Sciences, 16(3), 041–048. https://doi.org/10.30574/gscbps.2021.16.3.0262

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