Silver/chitosan/ascorbic acid nanocomposites ameliorate diabetic nephropathy in the model of type 1 diabetes

Esraa Abu El Qassem  Mahmoud; Ayman S  Mohamed; Sohair R  Fahmy; Amel Mahmoud  Soliman; Khadiga  Gaafar

doi:10.30574/gscbps.2021.16.3.0263

Authors

Esraa Abu El Qassem Mahmoud Zoology Department - Faculty of Science - Cairo University- 2613, Giza, Egypt.
Ayman S Mohamed Zoology Department - Faculty of Science - Cairo University- 2613, Giza, Egypt.
Sohair R Fahmy Zoology Department - Faculty of Science - Cairo University- 2613, Giza, Egypt.
Amel Mahmoud Soliman Zoology Department - Faculty of Science - Cairo University- 2613, Giza, Egypt.
Khadiga Gaafar Zoology Department - Faculty of Science - Cairo University- 2613, Giza, Egypt.

DOI:

https://doi.org/10.30574/gscbps.2021.16.3.0263

Keywords:

Type 1 Diabetes, AgNPs/chitosan/ascorbic acid nanocomposites, Rats, Kidney functions, Oxidative stress

Abstract

Aims: The present study aimed to evaluate anti-diabetic properties of AgNPs/chitosan/ascorbic acid nanocomposites (Ag-NCs) in streptozotocin-induced diabetic rats.

Main methods: Eighteen male Wistar albino rats were divided into three main groups (6 rats/group); control, diabetic and Ag-NCs groups. Control group: after a single dose of citrate buffer (0.1 mol/l, i.p), the rats orally received 1 ml distilled water daily for four weeks. The diabetic model was induced by a single dose of streptozotocin (60 mg/kg, i.p) for type 1diabetes. Diabetic groups were treated orally with and Ag-NCs (0.25mg/Kg body weight) daily for four weeks.

Key findings: AgNPs/chitosan/ascorbic acid nanocomposite group showed a reduction in the concentrations of glucose, NO, MDA, creatinine, urea and uric acid. At the same time, it appeared a general increase in insulin, CAT, and SOD activities and GSH concentration. The histopathological investigation illustrated a clear improvement in renal architecture.

Significance: The suggested mechanism of action for Ag-NCs in decreasing diabetic nephropathy includes two pathways; the hypoglycemic activity and the antioxidant role of Ag-NCs

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