Isolation and characterization of phosphate solubilizing bacteria in saline soil from Costal Region of Odisha

Bandita  Pati; Sanhita  Padhi

doi:10.30574/gscbps.2021.16.3.0273

Authors

Bandita Pati Department of Botany and Biotechnology, Ravenshaw University, Cuttack 753003, Odisha, India.
Sanhita Padhi Department of Botany and Biotechnology, Ravenshaw University, Cuttack 753003, Odisha, India.

DOI:

https://doi.org/10.30574/gscbps.2021.16.3.0273

Keywords:

Phosphate solubilizing ability, Halo zone, PSB, Solubilizing index, Acid phosphatase

Abstract

The present study was conducted to isolate phosphate solubilizing bacteria (PSB) from rhizospheric saline soils of coastal Odisha, India and evaluated their phosphate solubilizing ability. Total four PSB were isolated based on the halo zone formation (solubilizing index 2.63-3.14) on PVK agar medium and were characterized based on biochemical and molecular characteristics as Bacillus subtilis (B1), B. megaterium (B2), Sphingomonas paucimobilis (P2) and Kocuria kristinae (P6). The inorganic phosphate released by PSB ranged from 18.532 to 38.250µg/ml with decreasing the pH PVK broth up to 3.9. Acid phosphatase activity for PSB were recorded 84.237-98.658µmol/min. Glucose was found to be the best carbon source for B. subtilis, Sphingomonas paucimobilis and Kocuria kristinae whereas mannitol for B. megaterium. Optimum acid phosphatase activity was observed for all the four PSB isolates in presence of ammonium sulphate as nitrogen source in PVK broth at 30oC and pH 7.0.

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