Utilization of cell proliferation markers to diagnose cystic jaw pathologies

Heba Ahmed  Elhendawy; Heba Allah Ibrahim  Taher; Nadia Mostafa  Lotfy

doi:10.30574/gscbps.2021.16.3.0274

Authors

Heba Ahmed Elhendawy Oral Pathology Department, Faculty of Dentistry, Mansoura University, Egypt.
Heba Allah Ibrahim Taher Oral Pathology Department, Faculty of Dentistry, Mansoura University, Egypt.
Nadia Mostafa Lotfy Oral Pathology Department, Faculty of Dentistry, Mansoura University, Egypt.

DOI:

https://doi.org/10.30574/gscbps.2021.16.3.0274

Keywords:

Biological and Pharmaceutical Sciences, Unicystic ameloblastoma (UA), PCNA, MCM3, Cell proliferation markers

Abstract

Markers of cell proliferation are widely used as diagnostic and prognostic tools. Coincident estimation of these markers increases the precise evaluation of the proliferative status of different tissues and can also be helpful in determining progression, aggressiveness and prognosis of the lesions. The current study investigated the expression of PCNA and MCM3 cell proliferation markers in 40 formalin fixed paraffin embedded tissue blocks of odontogenic keratocyst (OKC) and unicystic ameloblastoma (UA) cases using immunohistochemistry method. Markers` expression based on the intensity, percentage of positively stained cells and localization of reaction through the cyst lining epithelium was separately analyzed for each marker using Chi square test, the results of which were significant for the two markers (P < 0.05). Both markers revealed statistically significant differences between OKC and UA cases regarding markers expression intensity, positivity score and localization of reaction through the epithelium. Mural UA histologic variant was significantly different than luminal and intraluminal variants. The correlation coefficient between the two markers was found to be 0.86.

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References

RL Avelar, AA Antunes, RWF Carvalho, PGCF Bezerra, PJ Oliveira Neto, ESS Andrade. Odontogenic cysts: a clinicopathological study of 507 cases., J. Oral Sci. 2009; 51(4): 581–586.

JR Bruno. Oral and Maxillofacial Pathology: A Rationale for Diagnosis and Treatment, Ann. Plast. Surg. 2003; 51(3): 333.

JC De-Vicente, A Torre-Iturraspe, AM Gutiérrez, P Lequerica-Fernández. Immunohistochemical comparative study of the odontogenic keratocysts and other odontogenic lesions, Med. Oral Patol. Oral Cir. Bucal. 2010; 15(5).

RM Browne. The pathogenesis of odontogenic cysts: a review, J. Oral Pathol. Med. 1975; 4(1): 31–46.

JM Wright, M Vered. Update from the 4th Edition of the World Health Organization Classification of Head and Neck Tumours: Odontogenic and Maxillofacial Bone Tumors, Head Neck Pathol. 2017; 11(1): 68–77.

P DeVilliers, et al. Calretinin Expression in the Differential Diagnosis of Human Ameloblastoma and Keratocystic Odontogenic Tumor, Am. J. Surg. Pathol. 2008; 32(2): 256–260.

M Tsuneki, J Cheng, S Maruyama, H Ida-Yonemochi, M Nakajima, T Saku. Perlecan-rich epithelial linings as a background of proliferative potentials of keratocystic odontogenic tumor, J. Oral Pathol. Med. 2008; 37(5): 287–293.

Z Jaafari-Ashkavandi, B Geramizadeh, MA Ranjbar. P63 and Ki-67 Expression in Dentigerous Cyst and Ameloblastomas., J. Dent. (Shiraz, Iran), 2015; 16(4): 323–8.

WM Mendenhall, JW Werning, R Fernandes, RS Malyapa, NP Mendenhall. Ameloblastoma, Am. J. Clin. Oncol. 2007; 30(6): 645–648.

A Hussain, DP Murray. Modified submandibular sialoadenectomy, Ear, Nose Throat J. 2004; 83(11): 768–771.

S Ghattamaneni, S Nallamala, VR Guttikonda. Unicystic ameloblastoma in conjunction with peripheral ameloblastoma: A unique case report presenting with diverse histological patterns, J. Oral Maxillofac. Pathol. 2017; 21(2): 267–272.

HP Philipsen, PA Reichart. Unicystic ameloblastoma. A review of 193 cases from the literature, Oral Oncology. 1998; 34(5): 317–325.

Neville BW, Damm DD, Allen C, Chi AC. Oral and maxillofacial pathology. Elsevier Health Sciences. 2015 May 13.

H Coleman, M Altini, H Ali, C Doglioni, G Favia, E Maiorano. Use of calretinin in the differential diagnosis of unicystic ameloblastomas, Histopathology. 2001; 38(4): 312–317.

RCK Jordan, PM Speight. Current concepts of odontogenic tumours, Diagnostic Histopathol. 2009; 15(6): 303–310.

F Titinchi, CJ Nortje. Keratocystic odontogenic tumor: A recurrence analysis of clinical and radiographic parameters, Oral Surg. Oral Med. Oral Pathol. Oral Radiol. 2012; 114(1): 136–142.

Carreón-Burciaga RG, González-González R, Molina-Frechero N, Bologna-Molina R. Immunoexpression of Ki-67, MCM2, and MCM3 in ameloblastoma and ameloblastic carcinoma and their correlations with clinical and histopathological patterns. Disease markers. 2015 Jan 1;2015.

Güler N, Çomunoğlu N, Cabbar F. Ki-67 and MCM-2 in dental follicle and odontogenic cysts: the effects of inflammation on proliferative markers. The Scientific World Journal. 2012 Jan 1;2012

Scholzen T, Gerdes J. The Ki-67 protein: from the known and the unknown. J Cell Physiol 2000; 182: 311-22.

Essers J, Theil AF, Baldeyron C, et al. Nuclear dynamics of PCNA in DNA replication and repair. Mol Cell Biol. 2005; 25: 9350-9.

De Biasio A, Sanchez R, Prieto J, et al. Reduced stability and increased dynamics in the human proliferating cell nuclear antigen (PCNA) relative to the yeast homolog. Plos One. 2011; 6: e16600.

JJ Blow, B Hodgson. Replication licensing--defining the proliferative state?, Trends Cell Biol. 2002; 12(2): 72–8.

JJ Blow, A Dutta. Preventing re-replication of chromosomal DNA, Nature Reviews Molecular Cell Biology. 2005; 6(6): 476–486.

M Deraco, A Cabras, D Baratti, S Kusamura. Immunohistochemical Evaluation of Minichromosome Maintenance Protein 7 (MCM7), Topoisomerase IIα, and Ki-67 in Diffuse Malignant Peritoneal Mesothelioma Patients Using Tissue Microarray, Ann. Surg. Oncol. 2015; 22(13): 4344–4351.

ÁCG Henriques, MG Vasconcelos, HC Galvão, LB De Souza, R De Almeida Freitas. Comparative analysis of the immunohistochemical expression of collagen IV, MMP-9, and TIMP-2 in odontogenic cysts and tumors, Oral Surgery, Oral Med. Oral Pathol. Oral Radiol. Endodontology. 2011; 112(4): 468–475.

Gnepp DR. Diagnostic surgical pathology of the head and neck e-book. Elsevier Health Sciences. 2009.

DG Gardner. Plexiform unicystic ameloblastoma: A diagnostic problem in dentigerous cysts, Cancer. 1981; 47(6): 1358–1363.

A Abdel-Aziz, MM Amin. EGFR, CD10 and proliferation marker Ki67 expression in ameloblastoma: Possible role in local recurrence, Diagn. Pathol. 2012; 7(1).

Salehinejad J, Zare-Mahmoodabadi R, Saghafi S, et al. Immunohistochemical detection of p53 and PCNA in ameloblastoma and adenomatoid odontogenic tumor. J Oral Sci. 2011; 53: 213-7.

Ahmed MM, El-Azab SM. Evaluation of cell cycle-related indicators in plexiform amelo-blastoma. J Egypt Natl Canc Inst. 2008; 20: 294-301.

Saghafi S, Zare-Mahmoodabadi R, Salehinejad J, et al. Immunohistochemical analysis of p53 and PCNA expression in calcifying odontogenic cyst. J Oral Sci. 2010; 52: 609-13.

W Thosaporn, A Iamaroon, S Pongsiriwet, KH Ng. A comparative study of epithelial cell proliferation between the odontogenic keratocyst, orthokeratinized odontogenic cyst, dentigerous cyst, and ameloblastoma, Oral Dis. 2004; 10(1): 22–26.

ZJ Ashkavandi, AD Najvani, AA Tadbir, S Pardis, MA Ranjbar, MJ Ashraf. MCM3 as a novel diagnostic marker in benign and malignant salivary gland tumors,” Asian Pacific J. Cancer Prev. 2013; 14(6): 3479–3482.

LDF Valverde et al. MCM3: A Novel Proliferation Marker in Oral Squamous Cell Carcinoma, Appl. Immunohistochem. Mol. Morphol. 2018; 26(2): 120–125.

Z Jaafari-Ashkavandi, F Mehranmehr, E Roosta. MCM3 and Ki67 proliferation markers in odontogenic cysts and ameloblastoma,” J. Oral Biol. Craniofacial Res. 2019; 9(1): 47–50.

N MR, F ER, SS YT, P DE. Syndecan-1 (CD138) and Ki-67 expression in odontogenic cystic lesions,” Braz. Dent. J. 2011; 22(3): 223–229.

S Nafarzadeh, M Seyedmajidi, S Jafari, A Bijani, A Rostami-Sarokolaei. A comparative study of PCNA and Ki-67 expression in dental follicle, dentigerous cyst, unicystic ameloblastoma and ameloblastoma, Int. J. Mol. Cell. Med. 2013; 2(1).

Coşarcă, A.S., Mocan, S.L., Păcurar, M.A.R.I.A.N.A., Fülöp, E.M.Ő.K.E. and Ormenişan, A.L.I.N.A., 2016. The evaluation of Ki67, p53, MCM3 and PCNA immunoexpressions at the level of the dental follicle of impacted teeth, dentigerous cysts and keratocystic odontogenic tumors. Romanian journal of morphology and embryology= Revue roumaine de morphologie et embryologie, 57(2), pp.407-412.

M Shear. The aggressive nature of the odontogenic keratocyst: is it a benign cystic neoplasm?Part 2. Proliferation and genetic studies, Oral Oncol. 2002; 38(4): 323–331.

AG Lameira et al. MCM3 could be a better marker than Ki-67 for evaluation of dysplastic oral lesions: an immunohistochemical study, J. Oral Pathol. Med. 2014; 43(6): 427–434.

E Endl, I Kausch, M Baack, R Knippers, J Gerdes, T Scholzen. The expression of Ki-67, MCM3, and p27 defines distinct subsets of proliferating, resting, and differentiated cells,” J. Pathol. 2001; 195(4): 457–462.

YS Lee et al. Minichromosome maintenance protein 3 is a candidate proliferation marker in papillary thyroid carcinoma, Exp. Mol. Pathol. 2010; 88(1): 138–142.

KM Lau, QKY. Chan, JCS Pang et al. Minichromosome maintenance proteins 2, 3 and 7 in medulloblastoma: overexpression and involvement in regulation of cell migration and invasion, Oncogene, 2010; 29(40): 5475–5489.

Otero D, Lourenco SQC, Avila R, Bravo M, Sousa T, Faria PAS, et al. Expression of proliferative markers in ameloblastomas and malignant odontogenic tumors. Oral Dis. 2013; 19: 360–5.

Shahela T, Aesha S, Ranganathan K, Rooban T, Uma Devi Rao K, Joshua E, et al. Immunohistochemical expression of PCNA in epithelial linings of selected odontogenic lesions. J Clin Diagn Res. 2013; 7(11): 2615–8.

Gonzalez-Gonzalez R, Carreón-Burciaga RG, Molina-Frechero N, Bologna-Molina R. Correlation between proliferation markers: MCM-2, MCM-3 and Ki-67 in Ameloblastoma and Ameloblastic carcinoma. Oral Oncol. 2013; 49: S93–156.

H Yan, AM Merchant, BK Tye. Cell cycle-regulated nuclear localization of MCM2 and MCM3, which are required for the initiation of DNA synthesis at chromosomal replication origins in yeast., Genes Dev. 1993; 7(11): 2149–2160.

STM, MS, OV. Expressions of bax, bcl-2 and Ki-67 in odontogenic keratocysts (Keratocystic Odontogenic Tumor) in comparison with ameloblastomas and radicular cysts, Turk Patoloji Derg. 2012; 28(1): 49–55.

H Kumamoto, N Takahashi, K Ooya. K-Ras gene status and expression of Ras/mitogen-activated protein kinase (MAPK) signaling molecules in ameloblastomas, J. Oral Pathol. Med. 2004; 33(6): 360–367.

ZY, WL, CXM. [Expression of p-p38MARK, uPA and Ki-67 in epithelial odontogenic tumour]., Zhonghua Kou Qiang Yi Xue Za Zhi. 2010; 45(9): 535–539.

MA González Moles et al. Cell proliferation associated with actions of the substance P/NK-1 receptor complex in keratocystic odontogenic tumours, Oral Oncol. 2008; 44(12): 1127–1133.

Munoz M, Covenas R. Neurokinin-1 receptor: a new promising target in the treatment of cancer. Discovery medicine. 2010; 10(53): 305-13.