Influence of two phosphate-potassium solubilizing bacterial species on biomass and nitrate concentration on mustard greens (Brassica juncea (L.) Czernjaew) cultivated on acid sulfate soils

Authors

  • Tran Duy Phat Department of Agricultural Technology, College of Rural Development, Can Tho University, Vietnam
  • Cao Ngoc Diep Biotechnology Microbiology Department, Biotechnology R&D Institute, Can Tho University, Vietnam

DOI:

https://doi.org/10.30574/gscbps.2021.16.3.0278

Keywords:

Acid sulfate soil, Biomass yield, Brassica juncea, Nitrogen chemical fertilizer, Nitrate in leaf, P-K solubilizing bacteria

Abstract

Two field trials were conducted to determine the effect of P-K solubilizing bacteria and nitrogen chemical fertilizer on growth, yield and nitrate in leaf of mustard greens Brassica juncea L.) cultivated on acid sulfate soils. Eight P-K solubilizing bacterial strains composed of 3 strains of Acinetobacter calcoaceticus and 5 strains of Rhizobium sp., bacterial liquid were directly watered into plant at 3 stages [6, 12 and 21 days after planting] during vegetable cultivation, chemical fertilizer (60N – 40 P2O5 – 20 K2O) and control (no-inoculation). The study revealed that eight P-K solubilizing bacterial strains have good characteristics as nitrogen fixation, phosphate and potassium solubilisation. Application of nitrogen chemical concentration, increasing nitrate concentration of leaf of mustard greens. Application of bacterial liquid with Acinetobacter calcoaceticus strain NT4 and Acinetobacter calcoaceticus strain NT30 strain, Rhizobium tropici strain N18 and Rhizobium leguminosarum strain K35 on mustard greens cultivated on acid sulfate soils (basal fertilizer: 40 P2O5 – 20 K2O) was the best cultural practice because the this model not only supported the highest biomass, but also decreased nitrate concentration in leaf of mustard greens.

Metrics

Metrics Loading ...

References

Kumar NKS, Sowmyamala BV, Sadhan Kumar PG, Vasudev PN, Vasantha Kumar V, Nagaraj HT. Effect of Plant Growth Promoting Rhizobacteria (PGPR) on Growth and Yield of Bitter Gourd. International J. of Applied Biology and Pharmaceutical Technology. 2012; (3)1: 1-7.

Ahmad D, Ahmad I, Khan MS. Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microbiol. Res. 2008; (63): 173-81.

Lại Chi Quoc, Nguyen Thị Don, Cao Ngoc Diep. Selection and identification of nitrogen fixing bacteria (phosphorus and potassium solubilizing) isolated from weathered material of granite rock of Cam mountain An Giang province. Journal of Science, Can Tho University. 2012; 24a: 60-69.

Nguyen Thi Don, Cao Ngoc Diep. Effect of P-K solubilizing bacteria and nitrogen chemical fertilizer on Growth, Yield and Nitrate leaf of Brassica juncea L. cultivated on acid sulfate soils. International Journal of Innovations in Engineering and Technology (IJIET). 2019; 14(1): 020-028.

Martinez-Romero E, Segovia L, Mercante FM, Franco AA, Graham P, Pardo MA. Rhizobium tropici, a novel species nodulating Phaseolus vulgaris L. beans and Leucaena sp. trees. Int. J. Syst. Bacteriol. 1991; 41: 417–426.

de Vasconcelos MA, Cunha CO, Arruda FVS, Carneiro VA, Bastos RM, Mercante FM, do Nascimento KS, Cavada BS, dos Santos RP, Teixeira EH. Effect of Leguminous Lectins on the Growth of Rhizobium tropici CIAT899; Molecules. 2013; 18: 5792-5803.

Ribeiro RA, Rogel MA, López-López A, Ormenõ-Orrillo E, Barcellos FG, Martínez J, Thompson FL, Martínez-Romero E, Hungria M. Reclassification of Rhizobium tropici type A strains as Rhizobium leucaenae sp. nov. Int. J. Syst. Evol. Microbiol. 2012; 62: 1179–1184.

Chueire LMO, Bangel EV, Mostasso FL, Campo RJ, Pedrosa FO, Hungria M. Taxonomic classification of rhizobial strains recommended for soybean and common bean crops in Brazil based on the sequencing of the 16s rRNA gene. Rev. Bras. Ciênc. Solo. 2003; 27: 833–840.

Chen L, Yuan J, Xu Y, Zhang F, Chen Z. Comparison of clinical manifestations and antibiotic resistances among three genospecies of the Acinetobacter calcoaceticus- Acinetobacter baumannii complex PLOS ONE. 1 February 2018; 1: 1-11.

Binh PT, Tru NV, Dung, VTT, Thoa NT, Thao PV, et al. Bacteria in Wooden Box Fermentation of Cocoa in Daklak, The Western Highland Agriculture and Forestry Science Institute, Vietnam. J Microbiol Exp. 2017; 5(7): 00176.

Kudpeng K, Thayanukul P, Thiravetyan P. Bioleaching of Gold from Silicate Ore by Macrococcus caseolyticus and Acinetobacter calcoaceticus: Effect of Medium, Amino Acids and Growth Supernatant. Minerals. 2021; 11: 580.

Tran Thị Giang, Nguyen Thị Quyên, Cao Ngọc Điệp. Isolation and identification of PGPR isolated from leaf-eating vegetables cultivating at Can Tho city. The journal of Science, Can Tho University, 2014, 35: 65-73.

Nguyen Thi Don, Cao Ngoc Diep. Isolation, characterization and identification of phosphate- and potassium solubilizing bacteria from weathered materials of granite rock mountain, That Son, An Giang province, Vietnam. American Journal of Life Sciences. 2014: 2(5): 282-291.

doi: 10.11648/j.ajls.20140204.16 (online). ISSN 2328 – 5737.

Nguyen Thi Lan Huong. Determination of the correlation between N concentration in the soil and nitrate concentration stimulated in some kinds of vegetable.” Journal of The Earth Science. 2013; 35(4): 418-423.

Antoun H, Beauchamp CJ, Goussard R, Chabot R, Lalande R. Potential of Rhizobium and Bradyrhizobium species as plant growth promoting rhizobacteria on nonlegumes: Effect on radishes (Raphanus sativus L.). Plant and Soil. 1998; 204: 57 – 67.

Downloads

Published

2021-09-30

How to Cite

Phat, T. D. ., & Diep, C. N. . (2021). Influence of two phosphate-potassium solubilizing bacterial species on biomass and nitrate concentration on mustard greens (Brassica juncea (L.) Czernjaew) cultivated on acid sulfate soils. GSC Biological and Pharmaceutical Sciences, 16(3), 155–163. https://doi.org/10.30574/gscbps.2021.16.3.0278

Issue

Section

Original Article