Antimicrobial and Antioxidant properties of medicinal mushroom Ganoderma P. Karst

Authors

  • Anjali Suansia Odisha University of Agriculture and Technology, Bhubaneswar- 751003.
  • Priya John N. M. College of Agriculture, Navsari Agricultural University, Navsari- 396450.

DOI:

https://doi.org/10.30574/gscbps.2021.17.2.0326

Keywords:

Extract, Antifungal activity, Antibacterial activity, DPPH, Ferrous ion

Abstract

Ganoderma species have been known all over the world as highly medicinal mushrooms. Antimicrobial activity of it is an attractive approach which raises the global interests from the scientific community. In this study, the antimicrobial assay of ethanol and methanol extracts of Ganoderma were prepared by using the dried mycelial powder obtained from five different liquid media; was performed against seven plant pathogenic fungi viz., Alternaria macrospora, Aspergilus niger, A. flavus, Colletotrichum falcatum, Fusarium oxysporum, Pestalotiopsis mangiferae and Penicillium sp. and two plant pathogenic bacteria Xanthomonas oryzae and Ralstonia solanacearum. The extracts of mycelia obtained from Yeast Wine Media exhibited the highest inhibition percentage as compared to rest. At concentration 1000ppm, there was complete inhibition of mycelial growth for Alternaria macrospora, Aspergilus niger and A. flavus while for Fusarium oxysporum and Penicillum sp. complete inhibition was observed at 500ppm but for Colletotrichum falcatum and Pestalotiopsis mangiferae, more than 80% mycelial inhibition at concentration 1000ppm in both ethanol and methanol extracts. In the case of Xanthomonas oryzae and Ralstonia solanacearum, at concentration 1000ppm, methanol extract showed the highest inhibition zone (3.50mm, 3.75mm). Ganoderma exhibited antagonistic effect against plant pathogenic fungi could add to the interest of developing Ganoderma as a successful bioagent in the near future. Antioxidant activities were evaluated by using DPPH radical scavenging assay and Metal chelating activity on ferrous ions. The DPPH radical scavenging effect was detected in methanol extract (Inhibition% = 27.312%) was higher than that of the ethanol extract (Inhibition% = 24.79%) and also Ferrous ion chelating ability of methanol extract (Inhibition% =22.27%) was higher than the ethanol extract (Inhibition% =12.55%). It is clearly indicated that both methanol and ethanol extract of the Ganoderma show antioxidant properties and Ganoderma extracts act as an effective antioxidant agents.

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References

Jong SC, Birmingham JM, Medicinal benefit of the mushroom Ganoderma, Advances in Applied Microbiology. 1991; 37: 104-132.

Jonathan SG, Kigigha LT, Ohimain E, Evaluaton of the Inhibitory Potentials of Eight higher Nigerian Fungi against Pathogenic microorganisms, African Journal of Biomedical Reaserch. 2008; 11: 197-202.

Yoon SY, Eo SK, Kim YS, Lee CK, et al., Antimicrobial activity of Ganoderma extract alone and in combination with some antibiotics, Archives of Pharmacol. Reseach. 1994; 17(6): 438-442.

Wasser SP and Weis AL, Medicinal properties of substances occurring in higher basidiomycetes mushrooms current perspectives (Review), International Journal of Medicinal Mushroom. 1999; 1: 31-62.

Suansia A & John P, Morphological and cultural Characteristics of Different Collections of Medicinal White-Rot Bracket Fungi Ganoderma P. Karst., International Journal of Current Microbiology and Applied Science. 2020; 9(1): 2636-2644.

Kapoor P & Sharma BM, Studies on different growth parameters of Ganoderma lucidum. International Journal of Science, Environment and Technology. 2014; 3: 1515-1524.

Suansia A, John P, Mycelial biomass production of medicinal mushroom Ganoderma P. Karst. International Journal of Agricultural Sciences. 2021; 17 (1): 15-19.

Parkash V & Sharma A, In vitro efficacy of bracket fungi for their potential antimicrobial activity. Journal of microbiology, biotechnology and food sciences. 2016; 6: 818-822.

Prakash V, Aggarwal A, Sharma S, Gupta S, et al., Antifungal activity of Withania somnifera L. Dunal on some phytopathogenic fungi. Bulletin of the National Institute of Ecology, 2005; 15: 15-218.

Blois MS, Antioxidant determinations by the use of a stable free radical. Nature. 1958; 181: 1199-1200.

Celik GY, Onbasil D, Altinsoy B & Alli, H, In vitro antimicrobial and antioxidant properties of Ganoderma lucidum extracts grown in Turkey. European Journal of Medicinal Plants. 2014; 4: 709-722.

Decker EA, Welch B, Role of ferritin as a lipid oxidation catalyst in muscle food. Journal of Agricultural and Food Chemistry. 1990; 38: 674-677.

Singh J, Gupta S, Malviya S, Ahrwar B, In-vitro evaluation of antimicrobial activity of Ganoderma lucidum. International Journal of Advanced Research. 2014; 2: 460-466.

Fidler G, Butu A, Rodino S, Butu M, et al., Antioxidant activity, bioactive compounds and antimicrobial effect of mushrooms extracts. Animal Science and Biotechnologies. 2015; 72: 32-35.

Fan L, Li J, Deng K & Ai L, Effects of drying methods on the antioxidant activities of polysaccharides extracted from Ganoderma lucidum. Carbohydrate Polymers. 2012; 87: 1849-1854.

Mau JL, Tsai SY, Sung YH & Huang SJ, Antioxidant properties of hot water extracts from Ganoderma tsugae Murrill, Lebensmittel-Wissenschaft and Technologie. 2002; 38: 589-597.

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Published

2021-11-30

How to Cite

Suansia, A. ., & John, P. . (2021). Antimicrobial and Antioxidant properties of medicinal mushroom Ganoderma P. Karst. GSC Biological and Pharmaceutical Sciences, 17(2), 106–112. https://doi.org/10.30574/gscbps.2021.17.2.0326

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Original Article