Antimicrobial resistant in Gram-negative bacilli: Enterobacteriaceae and non-fermenting bacilli isolated at Sominé DOLO Hospital of Mopti, Mali

Moussa  Diawara; Modibo  Coulibaly; Dramane  Samaké; Soumaila  Touré; Dramane  Cissé; Abdoulaye  Traoré; Djibril  Traoré; Pierre  Coulibaly; Bréhima  Traoré; Oumar  Guindo; Bakary  Maiga; Amagana  Dolo

doi:10.30574/gscbps.2022.18.1.0021

Authors

Moussa Diawara Department of Biomedical laboratory, Military hospital infirmary of Kati, Mali.
Modibo Coulibaly Department of Biomedical laboratory, Sominé DOLO Hospital of Mopti, Mali.
Dramane Samaké Department of Medicine, Sominé DOLO Hospital of Mopti, Mali.
Soumaila Touré Department of Biomedical laboratory, Sominé DOLO Hospital of Mopti, Mali.
Dramane Cissé Department of General surgery, Sominé DOLO Hospital of Mopti, Mali.
Abdoulaye Traoré Department of Anesthesia and resuscitation, Sominé DOLO Hospital of Mopti, Mali.
Djibril Traoré Department of General surgery, Sominé DOLO Hospital of Mopti, Mali.
Pierre Coulibaly Department of Gynecology and obstetrics, Sominé DOLO Hospital of Mopti, Mali.
Bréhima Traoré Department of General surgery, Sominé DOLO Hospital of Mopti, Mali.
Oumar Guindo Department of Public Health, Sominé DOLO Hospital of Mopti, Mali.
Bakary Maiga Faculty of Medicine and Odonto-Stomatology, University of Technical and Technological Sciences, Mali.
Amagana Dolo Faculty of Pharmacy, University of Technical and Technological Sciences, Mali.

DOI:

https://doi.org/10.30574/gscbps.2022.18.1.0021

Keywords:

Gram-negative bacilli, Anti-microbial resistant, Rational use of antibiotics

Abstract

Background: β-lactams and carbapenems. are the major antibiotics used to treat gram-negative bacteria and non-fermenting bacilli. However, the increasing production of β-lactamase and carbapenemase limits the therapeutic options. Our study aims to determine the resistant phenotypes of these bacteria while describing their epidemiological aspect.

Material and Methods: This was a cross-sectional study by consecutive enrollment from January 2018 to December 2019 at Sominé DOLO Hospital of Mopti, Mali. We performed manual method for bacteria culture, identification and antibiotics sensitivity testing. The antibiotics sensitivity testing was accessed by the diffusion method according to CA-SFM/EUCAST (“Comité de l’Antibiogramme de la Société Française de Microbiologie” / European Committee on Antimicrobial Susceptibility Testing) recommendations V1.0 february 2018 and V2.0 may 2019 2019 V.2.0. May recommendation released in 2019. Data were analyzed by software R 4.0.3 GUI 1.73 Catalina build (7892).

Results: At all 904 samples were included in this study. Out of the 904 cultures, 297 sample (32.85%) were positive. The rates of enzymes production were as follow: Extended-spectrum ß-lactamase (ESBL) 56.42% (101/179), cepholosporinase hyperproduction (HCASE) 15.64% (28/179), cephalosporinase production (CASE) 6.14% (11/179), penicillinase hyperproduction (HP) 5.58% (10/179), carbapenemase production (CP) 6.14% (11/179) and savage strains 10.05% (18/179).

Conclusion: Our data showed a high prevalence of resistance to β-lactamins ß-talactamins and carbapenemes in gram-negative bacteria and non-fermenting bacillus bacilli. The A high level of β-lactamase and carbapenemase production by gram negative bacillus were also reported by others authors calls for the rational use of antibiotic in hospital setting.

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