Molecular diversity of multi-resistant and extended–spectrum beta lactamase–producing Escherichia coli from door handles in Lafia, central Nigeria

Paul Alumbugu Tsaku; Taibat Ibrahim; Istifanus Haruna Nkene; Rejoice Helma Abimiku; David Ishaleku; Grace Pennap; Yakubu Boyi Ngwai

doi:10.30574/gscbps.2019.8.1.0074

Authors

Paul Alumbugu Tsaku Department of Microbiology, Nasarawa State University, Keffi, Nigeria.
Taibat Ibrahim Department of Science Laboratory Technology, Nasarawa State University, Keffi, Nigeria.
Istifanus Haruna Nkene Department of Microbiology, Nasarawa State University, Keffi, Nigeria.
Rejoice Helma Abimiku Department of Microbiology, Nasarawa State University, Keffi, Nigeria.
David Ishaleku Department of Microbiology, Nasarawa State University, Keffi, Nigeria.
Grace Pennap Department of Microbiology, Nasarawa State University, Keffi, Nigeria.
Yakubu Boyi Ngwai Department of Microbiology, Nasarawa State University, Keffi, Nigeria.

DOI:

https://doi.org/10.30574/gscbps.2019.8.1.0074

Keywords:

Escherichia coli, Extended-spectrum beta-lactamase, blaTEM, blaSHV, blaCTX-M, Restriction fragment length polymorphism

Abstract

Human hands have been implicated as a major source of dissemination of pathogenic microorganisms through fomites. This study investigated the diversity of ESBL-producing in E. coli isolates from door handles using restriction fragment length polymorphism (RFLP). An occurrence of 77(19.25%) of E. coli out of 400 samples was recovered from different locations. The study of antibiotics resistance showed that ampicillin, ceftazidime, and streptomycin were predominantly resistant. Multiple antibiotics resistance index of ≥0.3 was recorded in 73(94.81%) of the isolates. A total of 40 resistant phenotypes were observed in this study, with AMP-AUG-CAZ-CRO-S-CIP-SXT-TE-C being the commonest. Twenty isolates were characterised as Multidrug resistant (MDR) phenotypes, followed by, pan drug resistance (PDR) and extensive resistance (XDR) phenotypes recorded in 12 and 8 isolates respectively. Thirty-six (36) ESBL-producers were identified out of which 14 harboured bla_TEM, while 5 and 9 were carriers of bla_SHV and bla_CTX-Mrespectively. Most of the isolates shared a common origin, as revealed by result of the RFLP. The outcome of this study suggests the need for improved personal hygiene and the need for all stakeholders to be proactive in curtailing the spread of resistant pathogens.

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References

Eckburg PB, Bik EM, Bernstein CN, Purdom E, Dethlefsen L and Sargent M. (2005). Diversity of the human intestinal microbial flora. Science, 308 (5728), 1635–8.

Tenaillon O, Skurnik D, Picard B and Denamur E. (2010). The population genetics of commensal Escherichia coli. Nature Reviews Microbiology, 8(3), 207–217.

Bentley R and Meganathan R. (1982). Biosynthesis of vitamin K (menaquinone) in bacteria. Microbiological Reviews, 46 (3), 241–80.

Tsaku PA, Ehinmidu JO and Mohammed SA. (2017). Antibiotic Susceptibility and Plasmid Profile of Escherichia coli from Door Handles in Two Tertiary Institutions in Nasarawa State, Nigeria. Journal of Advances in Microbiology, 3 (3), 1-13.

Hudault S, Guignot J and Servin AL. (2001). Escherichia coli strains colonising the gastrointestinal tract protect germfree mice against Salmonella typhimurium infection. Gut, 49 (1), 47–55.

Reid G, Howard J and Gan BS. (2001). Can bacterial interference prevent infection? Trends in Microbiology, 9 (9), 424–428.

Vogt RL and Dippold L. (2005). Escherichia coli O157:H7 outbreak associated with consumption of ground beef, June-July 2002. Public Health Reports (Washington, DC: 1974), 120 (2), 174–8.

CDC (2012). Escherichia coli. Centers for Disease Control and Prevention, National Center for Emerging and Zoonotic Infectious Diseases.

Feng P, Weagant S and Grant M. (2002). Enumeration of Escherichia coli and the Coliform Bacteria. Bacteriological Analytical Manual (8th ed.). FDA/Center for Food Safety & Applied Nutrition, Retrieved 2007-01-25.

Thompson J and Andrea R. (2007). E. coli Thrives in Beach Sands. Live Science.

Ishii S and Sadowsky MJ. (2008). Escherichia coli in the Environment: Implications for Water Quality and Human Health. Microbes and Environments, 23 (2), 101–8.

Raji MA, Jamal W, Ojemhen O and Rotimi VO. (2013). Point-surveillance of antibiotic resistance in Enterobacteriaceae isolates from patients in a Lagos Teaching Hospital, Nigeria. Journal of Infection and Public Health, 6, 431–7.

Ahmed OI, El-Hady SA, Ahmed TM and Ahmed IZ. (2013). Detection of bla SHV and bla CTX-M genes in ESBL producing Klebsiella pneumoniae isolated from Egyptian patients with suspected nosocomial infections. The Egyptian Journal of Medical Human Genetics, 14, 277–283.

Delgado YC, Barrigas PT, Astutilloa SO, Jaramillo AP and Ausili A. (2016). Detection and molecular characterization of b-lactamase genes in clinical isolates of Gram-negative bacteria in Southern Ecuador. Brazilian Journal of Infectious Diseases, 20(6), 627–30.

Ghafourian S, Sadeghifard N, Soheili S and Sekawi Z. (2015). Extended Spectrum Beta-lactamases: Definition, Classification and Epidemiology. Current Issues in Molecular Biology, 17, 11-22.

Gutkind GO, Di Conza J, Power P and Radice M. (2013). Beta-lactamase mediated resistance: a biochemical, epidemiological and genetic overview. Current Pharmaceutical Design, 19, 164–208.

Paterson DL and Bonomo R. (2005). Extended-spectrum beta-lactamases: a clinical update. Clinical Microbiology Review, 18, 657–86.

Shaikh S, Jamale F, Shazi S, Syed M, Danish R and Mohammad AK. (2015). Antibiotic resistance and extended spectrum beta-lactamases: Types, epidemiology and treatment. Saudi Journal of Biological Sciences, 22, 90–101.

Bonnet R. (2004). Growing group of extended-spectrum beta lactamases: the CTX-M enzymes. Antimicrobial Agents and Chemotherapy, 48, 1-14.

Canton R, Novais A and Valverde A. (2008). Prevalence and spread of extended-spectrum beta-lactamase-producing Enterobacteriaceae in Europe. Clinical Microbiology Infections, 14, 144–53.

Fang H, Ataker F, Hedin G and Dornbusch K. (2008). Molecular epidemiology of extended-spectrum beta-lactamases among Escherichia coli isolates collected in a Swedish hospital and its associated health care facilities from 2001 to 2006. Journal of Clinical Microbiology, 46, 707–712.

Lina TT, Khajanchi BK, Azmi IJ, Islam MA, Mahmood B, Akter M, Banik A, Alim R, Navarro A, Perez G, Cravioto A and Talukder KA. (2014). Phenotypic and Molecular Characterization of Extended-Spectrum Beta-Lactamase Producing Escherichia coli in Bangladesh. PLoS ONE, 9(10), e108735.

Cheesbrough M. (2000). Microbiological Tests. In: Cheesbrough, M., Ed., District Laboratory Practice in Tropical Countries, Part II, Low Priced Edition, Cambridge University Press, Cambridge, 105-130.

Clinical and Laboratory Standards Institute (2012). Performance Standards for Antimicrobial Susceptibility Testing. CLSI 22nd Informational Supplement M100-S22. Wayne, PA, USA.

Krumperman PH. (1983). Multiple antibiotic indexing Escherichia coli to identifying risk sources of faecal contamination of foods. Applied Environmental Microbiology, 46, 165-170.

Paul S, Bezbarauh RL, Roy MK and Ghosh AC. (1997). Multiple antibiotic resistant index and its reversion in Pseudomonas aeroginosa. Letters of Applied Microbiology, 24, 109-171.

Lacher DW, Steinsland H and Whittam TS. (2006). Allelic subtyping of the intimin locus (eae) of pathogenic Escherichia coli by fluorescent RFLP. FEMS Microbiology Letters, 261, 80–7.

Melika S, Reza M and Noor A. (2014). Molecular identification of TEM and SHV extended spectrum β-lactamase in clinical isolates of Acinetobacter baumannii from Tehran hospitals. Journal of Genes, Microbes and Immunity, 14, 1-9.

Afzali H, Firoozeh F, Amiri A, Moniri R and Zibaei M. (2015). Characterization of CTX-M-Type Extend-Spectrum β-Lactamase Producing Klebsiella spp. in Kashan, Iran. Jundishapur Journal of Microbiology, 8(10), e27967.

Jiang H, Dong H, Zhang G, Yu B, Chapman LR and Fields MW. (2006). Microbial diversity in water and sediment of Lake Chaka, an athalassohaline lake in northwestern China. Applied and Environmental Microbiology, 72(6), 3832–45.

Samy SA and Hamdy ME. (2012). Pathogenic Bacteria Associated with Different Public Environmental Sites in Mecca City. Open Journal of Medical Microbiology, 2, 133-137.

Oranusi S, Dahunsi SO, Owoso OO and Olatile T. (2013). Microbial profiles of Hands, Foods, Easy contact surfaces and Food contact surfaces: A case study of a University Campus. Novus International Journal of Biotechnology & Bioscience, 2(1), 30-38.

Lynn M, Vivian OA and Wasa AA. (2013). The prevalence of bacterial organisms on toilet door handles in Secondary Schools in Bokkos L. G. A., Jos, Plateau Sate, Nigeria. IOSR Journal of Pharmacy and Biological Sciences, 8(4), 85-91.

Augustino C, Asha L, Joel M, Alexanda M, Eliakunda M, Elisa M, Isaac M and Emma P. (2014). Determination of bacterial load and antibiotic susceptibility testing of bacteria isolated from students’ toilets at Sokoine University of Agriculture, Morogoro, Tanzania. Journal of Health, Medicine and Nursing, Vol. 5.

Ajayi A and Ekozien MI. (2014). Sensitivity Profile of Bacterial Flora Isolated From Bathroom. Elite Research Journal of Biotechnology and Microbiology, 2(1), 1 – 3.

Mohammed SA, Tsaku PA, Nkene IH, Oti VB and Ekeleme IK. (2017). Plasmid-Mediated Resistance in Salmonella typhi Isolated from Door Handles in Nasarawa State, North-Central, Nigeria. Asian Journal of Biotechnology and Bioresource Technology, 1 (1), 1-12.

Opere BO, Ojo JO, Omonighehin E and Bamidele M. (2013). Antibiotic Susceptibility and Plasmid Profile Analysis of Pathogenic Bacteria Isolated from Environmental Surfaces in Public Toilets. Transnational Journal of Science and Technology, 3(2), 22-30.

Okonko IO, Soleye FA, Amusan TA, Ogun AA, Ogunnusi TA and Ejembi J. (2009). Incidence of Multi-Drug Resistance (MDR) Organisms in Abeokuta, Southwestern Nigeria. Global Journal of Pharmacology, 3(2), 69-80.

Nahum GG, Uhl K and Kennedy DL. (2006). Antibiotics use in pregnancy and Lactation what is and is not known about teratogenics and toxic risks. Obstetrics and Gyneacology, 107 (5), 1120 -1138.

Norwitz ER and Greenberg JA. (2009). Antibiotics in Pregnancy: are they safe? Review of Obstetric Gyneacology, 2(3), 135-6.

Ngwai YB, Nkene IH, Egan CA, Osabo PP and Envuladu EY. (2013). Minimum Inhibitory concentration of cephalosporin antibiotics for Fecal and Urinary Escherichia coli from University student in Keffi, Nigeria. Nsuk Journal of Sciences and Technology, 3, 1-2.

Oteo J, Campos J and Baquero F. (2002). Antibiotic resistance in 1962. Invasive isolates of Escherichia coli in 27 Spanish hospitals participating in the European Antimicrobial Resistance Surveillance System (2001). Journal Antimicrobial Chemotherapy, 50, 945-952.

Sherley M, Gardon DM and Collingnon PJ. (2004). Evolution of multi-resistance plasmids in Australia clinical isolates of Escherichia coli. Microbiology, 150, 1539– 1546.

Piddock LJV. (2006). Clinically relevant chromosomally encoded multi drug resistance efflux pumps in bacteria. Clinical Microbiology Reviews, 19(2), 382-402.

Depardieu F, Podglajen I, Leclercq R, Collatz E. and Courvalin P. (2007). Modes and modulations of antibiotic resistance gene expression. Clinical Microbiology Reviews, 20(1), 79-114.

Leavitt A, Navon-Venezia S, Chmelnitsky I, Schwaber MJ and Carmeli Y. (2007). Emergence of KPC-2 and KPC-3 in carbapenem-resistant Klebsiella pneumoniae strains in an Israeli hospital. Antimicrobial Agents and Chemotherapy, 51(8), 3026-3029.

Lockhart SR, Abramson MA, Beekman SE, Gallagher G, Riedel SR, Diekma DJ, Quinn JP and Doern GV. (2007). Antimicrobial resistance among Gram-negative bacilli as causes of infections in intensive care unit patients in the United States between 1993 and 2004. Journal of Clinical Microbiology, 45, 3352-3359.

Akujobi CN and Ewuru CP. (2010). Detection of Extended Spectrum Beta-Lactamases in Gram Negative Bacilli from Clinical Specimens in a Teaching Hospital in South Eastern Nigeria. Nigerian Medical Journal, 51(4), 141-146.

Kamel NA, Aboshanab KM, Abouelwafa M and El-tayeb WN. (2013). Plasmid mediated extended spectrum beta-lactamase producing strains of enterobacteriaceae isolated from diabetic foot infections in Egypt. Archaeological Clinical Microbiology, 4(4), 1-8.

Inwezerua C, Mendonça N, Calhau V, Domingues S, Adeleke OE and Da Silva GJ. (2014). Occurrence of extended-spectrum beta-lactamases in human and bovine isolates of Escherichia coli from Oyo state, Nigeria. Journal of Infectious Diseases in Developing Countries, 8(6), 774-779.

Basma M, Hela H, Jihène J, Faouzia M, Nathalie G, Guillaume A and Adnene H. (2013). Molecular epidemiology of extended-spectrum beta-lactamase-producing Escherichia coli in Tunisia and characterization of their virulence factors and plasmid addiction systems. BMC Microbiology, 13(147), 2-9.

El bouamri MC, Arsalane L, Zerouali K, Katfyd K, El kamouni Y and Zouhair S. (2015). Molecular characterization of extended spectrum b-lactamase-producing Escherichia coli in a university hospital in Morocco, North Africa. African Journal of Urology, 21, 161-6.

Omar BA, Alfadel OO, Atif HA and Mogahid ME. (2013). Prevalence of TEM, SHV and CTXM genes in Escherichia coli and Klebsiella spp Urinary Isolates from Sudan with confirmed ESBL phenotype. Life Sciences Journal, 10(2), 191-195.

Okeke IN, Aboderin OA, Byarugaba DK, Ojo KK and Opintan JA. (2007). Growing problem of multidrug-resistant enteric pathogens in Africa. Emerging Infectious Diseases, 13(11), 1640-1646.

Shahi SK, Singh VK and Kumar A. (2013). Detection of Escherichia coli and Associated b-Lactamases Genes from Diabetic Foot Ulcers by Multiplex PCR and Molecular Modeling and Docking of SHV-1, TEM-1, and OXA-1 b-Lactamases with Clindamycin and Piperacillin-Tazobactam. PLoS ONE, 8(7), e68234.

Sidjabat HE, Paterson DL, Adams-Haduch JM, Ewan L, Pasculle AW, Muto CA, Tian GB and Doi Y. (2018). Molecular Epidemiology of CTX-M-Producing Escherichia coli Isolates at a Tertiary Medical Center in Western Pennsylvania. Antimicrobial Agents and Chemotherapy, 62(8), 1-6.

Bajpai T, Pandey M, Varma M and Bhatambare GS. (2017). Prevalence of TEM, SHV, and CTX-M Beta-Lactamase genes in the urinary isolates of a tertiary care hospital. Avicenna Journal of Medicine, 7(1), 12–16.

Miriagou V, Carattoli A and Fanning S. (2006). Antimicrobial resistance islands: resistance gene clusters in Salmonella chromosome and plasmids. Microbes and Infection, 8, 1923-1930.

Winokur PL, Brueggemann A, DeSalvo DL, Hoffmann L, Apley MD, Uhlenhopp EK, Pfaller MA and Doern GV. (2000). Animal and Human Multidrug-Resistant, Cephalosporin-Resistant Salmonella Isolates Expressing a Plasmid-Mediated CMY-2 AmpC β-Lactamase. Antimicrobial Agents Chemotherapy, 44(10), 2777–2783.

Carattoli A. (2003). Plasmid-Mediated Antimicrobial Resistance in Salmonella enterica. Current Issues in Molecular Biology, 5, 113-122.

Helms M, Simonsen J and Molbak K. (2004). Quinolone resistance associated with increased risk of invasive illness or death during infection with Salmonella serotype Typhimurium. Journal of Infectious Disease, 190 (9), 1652-1654.

Osman BÖ, Tosun I, Aydin F, Kiliç OA and Ertürk M. (2006). Carriage of Mobilizable Plasmid Mediated β-Lactamase Gene in Ampicillin-Resistant Escherichia coli strains with Origin of Normal Fecal Flora. Turkish Journal of Medical Sciences, 36 (5), 307-314.

Yah SC, Eghafona NO, Oranusi S and Abouo AM. (2007). Widespread plasmids resistance transfers genes among Proteus species in diabetic wounds of patients in the Ahmadu Bello University Teaching Hospital (ABUTH) Zaria. African Journal of Biotechnology, 6(15), 1757-1762.