Antibiotic resistance in Escherichia coli from urine of patients with suspected urinary tract infections accessing Dalhatu Araf Specialist Hospital, Lafia, Nigeria

Istifanus Haruna Nkene; Yakubu Boyi Ngwai; Bassey Enya Bassey; Grace Pennap; Danladi Makwin Makut; Rejoice Helma Abimiku; Taibat Ibrahim; Paul Alumbugu Tsaku

doi:10.30574/gscbps.2019.8.2.0146

Authors

Istifanus Haruna Nkene Department of Microbiology, Nasarawa State University, P.M.B 1022, Keffi, Nasarawa State, Nigeria.
Yakubu Boyi Ngwai Department of Microbiology, Nasarawa State University, P.M.B 1022, Keffi, Nasarawa State, Nigeria.
Bassey Enya Bassey World Health Organization, Nigeria Country Office, UN House, Plot 617/618, Diplomatic Drive, Central Business District, P.M.B. 2861, Garki, Abuja, Nigeria
Grace Pennap Department of Microbiology, Nasarawa State University, P.M.B 1022, Keffi, Nasarawa State, Nigeria.
Danladi Makwin Makut Department of Microbiology, Nasarawa State University, P.M.B 1022, Keffi, Nasarawa State, Nigeria.
Rejoice Helma Abimiku Department of Microbiology, Nasarawa State University, P.M.B 1022, Keffi, Nasarawa State, Nigeria.
Taibat Ibrahim Department of Science Laboratory Technology, Nasarawa State University, P.M.B. 1022, Keffi, Nasarawa State, Nigeria.
Paul Alumbugu Tsaku Department of Microbiology, Nasarawa State University, P.M.B 1022, Keffi, Nasarawa State, Nigeria.

DOI:

https://doi.org/10.30574/gscbps.2019.8.2.0146

Keywords:

Escherichia coli, Urine, Antibiotics, Resistance

Abstract

The wide use of antibiotics to treat urinary tract infection (UTIs) caused by bacteria is compromised by the development of resistance mechanisms in bacteria. This study evaluated the antibiotic resistance in Escherichia coli from urine of patients with suspected UTI in Dalhatu Araf Specialist Hospital, Lafia, Nigeria. Four hundred urine samples of suspected UTI patients were collected and E. coli was isolated and identified using standard microbiological methods. Antimicrobial Susceptibility Testing for the isolates was carried out and interpreted as described by the Clinical and Laboratory Standards Institute (CLSI). The occurrence of the bacterium was 14.5 % (58/400). The occurrence was higher in female (15.8 %) than the male (12.5 %); and highest at > 50 years (31.8 %). The isolates were more resistant to ampicillin (79.3 %), streptomycin (62.0 %) and cefoxitin (58.6 %) but less resistant to gentamicin (15.8 %), imipenem (18.9 %) and amoxicillin/clavulanic acid (27.6 %). The occurrence of multi-drug resistance (MDR) isolates was 87.9%. The antibiotics namely gentamicin, imipenem and amoxicillin/clavulanic acid were very effective against the isolates and most of the isolates were MDR. There is thus a need for the hospital to limit its antibiotic use in the light of these observations.

Metrics

Metrics Loading ...

References

Nkene IH, Ngawi YB, Omede MU, Samuel J, Envuladu EY and Abimiku RH. (2015). Extended spectrum beta-lactamase producing Escherichia coli from urine of symptomatic and asymptomatic subjects in Keffi, Nigeria. International Journal of Research Studies in Biosciences, 3(12), 1-5.

Eghieye MO, Jodi SM, Bassey BE, Nkene IH, Abimiku RH and Ngwai YB. (2018). Antimicrobial resistance profile of Escherichia coli isolated from urine of patients in selected General Hospitals in Abuja Municipal, Nigeria. Asian Journal of Advanced Research and Report. 2(2), 1-10.

Mishra MP, Sarangi R and Padhy RN. (2016). Prevalence of multi-drug resistant uropathogenic bacterial in pediatric patients of Tertiary Care Hospital in Estern India. Journal of Infection and Public Health, 9(3), 308-314.

Niranjan V and Malim A. (2014). Antimicrobial resistance pattern in Escherichia coli causing Urinary Tract Infections among inpatients. Indian Journal of Medical Research, 139(4), 945-948.

Ferjani S, Saidani M, Amine FS and Boubaker IBB. (2015). Prevalence and characterization of plasmid-mediated quinolone resistance genes in extended-spectrum β-lactamase-producing Enterobacteriaceae in a Tunisian hospital. Microbial Drug Resistance, 21(2), 158–166.

Tajbakhsh E, Parvin A, Elham A, Nazila A and Faham K. (2016). Biofilm formation, antimicrobial susceptibility, serogroups and virulence genes of uropathogenic E. coli isolated from clinical samples in Iran. Antimicrobial Resistance and Infection Control, 5 (11), 2-8.

Olson RP, Harrel LJ and Kaye KS. (2009). Antimicrobial resistance in urinary Escherichia coli from college women with Urinary Tract Infections. Antimicrobial Agent and Chemotherapy, 53(3), 1285-1286.

Morill HJ, Morton JB, Caffery AR, Jiang L, Dosa D, Mormel LA and Laplente KI. (2017). Antimicrobial Resistance of Escherichia coli urinary isolates in Veterans affairs Health Care System. Antimicrobial Agent and Chemotherapy, 61(5), 02236-16.

Schito GC, Naber KG, Botto H, Palou J, Mazzei T and Gualco L. (2009). The ARESC study: an international survey on the antimicrobial resistanceof pathogens involved in uncomplicated urinary tract infections. International Journal of Antimicrobial Agents, 34, 407–13.

Cheesebrough M. (2006). Medical Laboratory Manual for Tropical Countries. Cambridge: Cambridge University Press, 49-97.

Clinical and Laboratory Standards Institute. (2017). Performance Standards for Antimicrobial Susceptibility Testing; 27nd Informational Supplement M100-S22. Wayne, Pa, USA.

Ngwai YB, Gyar SD, Pennap GRI, Makut MD, Ishaleku D, Corosi SM, Nkene IH and Uzoamaka N. (2014). Antibiogram of non-sorbitol fermenting Escherichia coli isolated from environmental sources in Keffi, Nigeria. NSUK Journal of Science and Technology, 4 (1&2), 152-163.

Magiorakos AP, Srinivasan A, Carey R, Carmeli Y, Falagas M, Giske C and Olsson‐Liljequist B. (2012). Multidrug‐resistant, extensively drug‐resistant and pandrug‐resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clinical microbiology and infection, 18(3), 268-281.

Rami′rez-Castillo FY, Moreno-Flores AC, Ave′lar-Gonza′lez F, Ma′rquez-Diaz F, Harel J and Guernero-Barrera AL (2018). An evaluation of Multi-drug resistance Escherichia coli isolates in Urinary Tract Infections from Aguascalients, Mexico; cross-sectional study. Annal Clinical Microbiology and Antimicrobial, 17, 1-13.

El-bouamri MC, Arsalane L, Zerouali K, Katfy K, El kamouni Y and Zouhair S. (2015). Molecular characterization of extended spectrum _lactamase-producing Escherichia coli in a university hospital in Morocco, North Africa. African Journal of Urology, 21, 161–166.

Eko KO, Jodi SM, Nkene IH, Abimiku RH, Ibrahim T and Ngwai YB. (2018). Antimicrobial Resistance Profile of Escherichia coli from urine of patients in Nagari Allah Magani Hospital, Keffi, Nigeria. Asian Journal of Research in Medical and Pharmaceutical Sciences, 5(4), 1-9.

Giwa FJ, Ige OT, Haruna DM, Yaqub Y, Lamido TZ and Usman SY. (2018). Extended-spectrum β-lactamase production and antimicrobial susceptibility pattern of uropathogens in a tertiary hospital in North-Western, Nigeria. Annals of Tropical Pathology, 9(1), 11-16.

Parajuli NP, Maharjan P and Parajul H. (2017). High rate of multi-drug resistance among uropathogenic Escherichia coli in children and analysis of Extended-spectrum β-lactamase producers from Nepal. Antimicrobial Resistance, Infection and Control, 6 (1), 9.

Hashemizadeh Z, Kalantar-Neyestanaki D and Mansouri S. (2018). Clonal relationship, antimicrobial susceptibility and molecular characterization of Escherichia coli isolates from urinary tract infection and feacal samples in South East Iran. Revista da Sociedade Brasileira de Medicina Tropical 51 (1), 11-19.