Bioremediation potentials of chicken droppings on crude oil polluted soil from automobile workshop

Danladi Makwin Makut; Olasumbo Opemipo Majekodunmi

doi:10.30574/gscbps.2019.8.2.0138

Authors

Danladi Makwin Makut Department of Microbiology, Nasarawa State University, P.M.B. 2022, Keffi, Nigeria.
Olasumbo Opemipo Majekodunmi Department of Microbiology, Nasarawa State University, P.M.B. 2022, Keffi, Nigeria.

DOI:

https://doi.org/10.30574/gscbps.2019.8.2.0138

Keywords:

Automobile workshops, Bioremediation, Chicken droppings, Total heterotrophic bacteria

Abstract

The bioremediation properties of chicken droppings on crude oil contaminated soil from automobile workshop in Keffi Nasarawa State of Nigeria were evaluated. Two experimental treatment (T1 and T2) with 10% and 30% chicken dropping addition to the contaminated soil were set up. A control (T3) was also set up consisting of contaminated soil only. The set up design was left for a period of 4 weeks during which the total heterotrophic bacteria (THB) count, total petroleum hydrocarbon degraded and gas chromatography and mass spectroscopy (GCMS) identification of the residue hydrocarbon after remediation were carried out. Soil samples remediated with 30% CD had significantly (p<0.05) the highest THB in the range of 6.45 x 10⁷ -11.23 x 10⁷ when compared with those remediated with 10% CD (5.34 x 10⁷ -8.36 x 10⁷). The non-remediated control had the least THB count of 3.45 ×10⁷ CFU/g. There was a rapid and progressive reduction in the total petroleum hydrocarbon during remediation in all the soil bio-remediated with 30 % chicken droppings compared to that of un-remediated soil. Automobile contaminated soil from Anguwan Kwara are more susceptible to remediation had the highest TPH degraded of 22, 39, 44 and 57.04%, at the end of the days 7, 14, 21 and 28 days respectively. GCMS revealed a total of 12,14,8,9 and 17 residual hydrocarbons in soil samples from Keffi Garage, New Keffi Hotel, Angwan Kwara, High Court and Angwan Tanko automobile workshops respectively after remediation with 30% chicken droppings while non-remediated soil recorded 22 residual hydrocarbons. The research shows that chicken droppings enhanced bioremediation of crude oil contaminated soil from automobile workshops.

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