Wound healing effects of Nigella sativa L. essential oil in streptozotocin induced in diabetic rats
DOI:
https://doi.org/10.30574/gscbps.2019.7.3.0095Keywords:
Diabetes mellitus, Essential oil, Nigella sativa L., Rat, Oxidative stressAbstract
Nigella sativa has been widely used in traditional Turkish medicine for several treatments, specially wounds and diabet disorders. However, the effects of this plant essential oil on wound healing have not yet been clearly explained. Thus study is required to develop new and effective treatment methods to deal with this subject. This present study was focused on utilization of Nigella sativa L. essential oil (NSE) on topical agent for diabetic wound treatment. A total of 72, Sprague–Dawley male rats were used in the present study. The rats were divided into nine groups (n=8). Streptozotocin (STZ) was given at single dose of 60 mg/kg/i.p. The animals showing diabetes (Blood glucose level >250 mg/dL) will be selected for wound groups. Wounds were created by punch (5 mm on dorsal region of each rat). On the 3th, 10th and 14th post-wounding day, the rats were sacrificed and dissected wound tissues. The results of this study showed that lipid peroxidation and oxidative stress significantly increased after STZ application. MDA and GSH levels, GPx, SOD and CAT activities were measured in plasma and wound tissues of the diabetic and treatment groups. While GSH, GPx, SOD and CAT levels in the plasma and wound tissues of the rats were decreased while MDA level was increased compared to the control group in the diabetes group. GSH, GPx, SOD and CAT levels were increased in the Nigella sativa L. essential oil treatments compared to the diabetes group while MDA level was decreased. All changes in biochemical parameters were directly proportionaled with histopathological changes of the wound tissues. NSE can be a play role of reducing of the lipid peroxidation, oxidative stress and associated complications and plays a beneficial role in the treatment of diabetic wound.
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Deshmukh PT and Gupta VB. (2013). Embelin accelerates cutaneous wound healing in diabetic rats. Journal of Asian Natural Products Research, 15(2), 158–165.
Kaur C and Kapoor HC. (2001). Antioxidants in fruits and vegetables- the millennium’s health. International Journal of Food Science&Technology, 36(7), 703–725.
Djeridane A, Yousfi M, Nadjemi B, Boutassouna D, Stocker P and Vidal N. (2006). Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chemistry, 97(4), 654–660.
Wound International. (2013). Best practice guidelines: wound management in diabetic foot ulcers.
Mariano R, Messora M, de Morais A, Nagata M, Furlaneto F, Avelino C, Paula F, Ferreira S, Pinheiro M and De Sene JP. (2010). Bone healing in critical-size defects treated with platelet-rich plasma: a histologic and histometric study in the calvaria of diabetic rat. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology and Endodontology, 109(1), 72–78.
Trivedi U, Parameswaran S, Armstrong A, Burgueno-Vega D, Griswold J and Dissanaike S and Rumbaugh KP. (2014). Prevalence of multiple antibiotic resistant infections in diabetic versus nondiabetic wounds. Journal of Pathogen.
Mirza RE and Koh TJ. (2015). Contributions of cell subsets to cytokine production during normal and impaired wound healing. Cytokine, 71(2), 409–412.
Sultan MT, Butt MS, Ahmad RS, Pasha I, Ahmad AN and Qayyum MM. (2012). Supplementation of Nigella sativa fixed and essential oil mediates potassium bromate induced oxidative stress and multiple organ toxicity. Pakistan Journal of Pharmacology Science, 25(1), 175–81.
Butt MS and Sultan MT. (2010). Nigella sativa reduces the risk of various maladies. Critical Reviews in Food Science and Nutrition, 50(7), 654–65.
Shabana A, El-Menyar A, Asim M, Al-Azzeh H and Al TH. (2013). Cardiovascular benefits of Black Cumin (Nigella sativa). Cardiovascular Toxicology, 13(1), 9–21.
Usanmaz Bozhüyük A, Kordalı̇ Ş and Bölük G. (2015). Satureja hortensis L. uçucu yağının antifungal etkisi. Ataturk Universitesi Ziraat Fakültesi Dergisi, 46(2), 107-112.
Lowry OH, Rosebrough NJ, Farr AL and Randall RJ. (1951). Protein measurement with the Folin phenol reagent. The Journal of Biological Chemistry, 193(1), 265-275.
Yoshioka T, Kawada K and Shimada T. (1979). Lipid peroxidation in materyal and cord blood and prodective mechanism against activated-oxygen toxicity in the blood. American Journal of Obstetrics& Gynecology, 135(3), 372-376.
Placer ZA, Cushman LL and Johnson BC. (1966). Esimaion of product of lipid peroxidaion (malonyl dialdehyde) in biochemical systems. Analytical Biochemistry, 16(2), 359-364.
Tietze F. (1969). Enzymic method for quantitavite determination of nanogram amounts of total and oxidized glutathione. Analytical Biochemistry, 27(3), 502-522.
Stahr HM. (1977). Analytical toxycology methods manual. Ames-Iowa, USA, Iowa State University Press, 32.
Matkovics B. (1988). Determination of enzyme activities in lipid peroxidation and glutathione pathways. Laboratoriumi Diagnosztika, 15, 248-249.
Sun Y, Oberley LW and Li Y. (1988). A simple method for clinical assay of superoxide dismutase. Clinical Chemistry, 34 (3), 497-500.
Goth L. (1991). A simple method for determenation of serum catalase activity and revision of serum catalase activity and revision of reference range. Clinica Chimica Acta, 196(2-3), 143-152.
Lerman OZ, Galiano RD, Armour M, Levine JP and Gurtner GC. (2003). Cellular dysfunction in the diabetic fibroblast: impairment in migration, vascular endothelial growth factor production, and response to hypoxia. The American Journal of Pathology, 162(1), 303–312.
Lan CC, Liu IH, Fang AH, Wen CH and Wu CS. (2008). Hyperglycaemic conditions decrease cultured keratinocyte mobility: implications for impaired wound healing in patients with diabetes. British Journal of Dermatology, 159(5), 1103–1115.
Loomans CJ, De Koning EJ, Staal FJ, Rookmaaker MB, Verseyden C, De Boer HC, VerhaarMC, Braam B, Rabelink TJ and Van Zonneveld AJ. (2004). Endothelial progenitor cell dysfunction a novel concept in the pathogenesis of vascular complications of Type 1 diabetes. Diabetes, 53(1), 195–199.
Khanna S, Biswas S, Shang Y, Collard E, Azad A, Kauh C, Bhasker V, Gordillo GM, Sen CK and Roy S. (2010). Macrophage dysfunction impairs resolution of inflammation in the wounds of diabetic mice. PLOS ONE, 5(3), e9539.
Nayak BS and Lexley MPP. (2006). Catharanthus roseus flower extract has wound-healing activity in Sprague Dawley rats. BMC Complementary and Alternative Medicine, 6, 41.
Arici M, Sagdic O and Gecgel U. (2005). Antibacterial effect of Turkish black cumin (Nigella sativa L.) oils. Grasas y Aceites, 56(4), 259-262.
Burits M and Bucar F. (2000). Antioxidant activity of Nigella sativa essential oil. Phytotherapy, 14(5), 323–328.
Baytop A. (1991). Farmasötik Botanik Ders Kitabı, İstanbul Üniv, 169.
Mahfouz M and El-Dakhakhny M. (1960). Isolation of a crystaline active principle from Nigella sativa L. seeds. Journal of Pharmacological Sciences UAR, 9(1), 9-19.
Varol Y. (2008). Investigation of the effects of Nigella sativa oil on cuta- neous wound healing in rats [Ph.D. Thesis], Marmara Universitesi Saglik Bilimleri Enstitusu, Istanbul, Turkey.
Roy K, Shivakumar H and Sarkar S. (2009). Wound healing potential of leaf extracts of Ficus religiosa on Wistar Albino strain rats. International Journal of PharmTech Research, 1(3), 506–508.
Yaman I, Durmus AS, Ceribasi S and Yaman M. (2010). Efects of Nigella sativa and silver sulfadiazine on burn wound healing in rats. Veterinaria Medicina, 55(12), 619–624.
Ab Rahman MR, Abdul Razak F and Mohd Bakri M. (2014). Evaluation of wound closure activity of Nigella sativa, Melastoma malabathricum, Pluchea indica and Piper sarmentosum extracts on scratched monolayer of human gingival fibroblasts. Evidence Based Complementary and Alternative Medicine, 190342.
Javadi SMR, Hashemi M, Mohammadi Y, MamMohammadi A, Sharifi A and Makarchian HR. (2018). Synergistic effect of honey and Nigella sativa on wound healing in rats. Acta Cirurgica Brasileira, 33(6), 518-523.
Sarkhail P, Esmaily H, Baghaei A, Shafiee A, Abdollahi M, Khademi Y, Madandar M and Sarkheil P. (2011). Burn healing potential of Nigella sativa seed oil in rats. International Journal of Pharmaceutical Sciences&Research, 2(1), 34-40.
Paheerathan V, Piratheepkumar R and Kumaran K. (2017). Evaluation of wound healing activity of Nigella sativa seed powder on Wistar albino rats. Scholarly Journal of Biological Science, 6(3), 60-63.
Aydin MS, Kocarslan A, Kocarslan S, Kucuk A, Eser İ, Sezen H, Buyukfirat E and Hazar A. (2015). Thymoquinone protects end organs from abdominal aorta ischemia/reperfusion injury in a rat model. Revista Brasileira de Cirurgia Cardiovascular, 30(1), 77–83.
Zareian P, Zahiri SH, Ketabchi F and Ruzmeh SH. (2007). Effect of local Tamarix monnifera on skin wound healing process in rabbit. Journal of Mazandaran University of Medical Sciences, 17(57), 48–57.
Sari Y, Kurniawan DW, Saryono, Arington IG and Toshi N. (2013). Nigella sativa gel improves granulation and reepithelialization tissue of diabetic rats. Paper presented at: International Conference on Sustainable Rural Development; August 23-24, Purwokerto, Indonesia.
Sari Y, Purnawan I, Kurniawan DW and Sutrisna E. (2018). A comparative study of the effects of Nigella sativa oil gel and aloe vera gel on wound healing in diabetic rats. Journal of Evidence-Based Integrative Medicine, 23.
Al-Douri AS and Al-Kazaz S. (2010). The effect of Nigella sativa oil (black seed) on the healing of chemically induced oral ulcer in rabbit. Al-Rafidain Dental Journal, 10(1), 151-157.
Xuan YH, Huang BB, Tian HS, Chi LS, Duan YM, Wang X, Zhu ZX, Cai WH, Zhu YT, Wei TM, Ye HB, Cong WT and Jin LT. (2014). High-glucose inhibits human fibroblast cell migration in wound healing via repression of bFGF-regulating JNK phosphorylation. PLOS ONE, 9, e108182.
Zia-Ul-Haq M, Ahmad S, Bukhari SA, Amarowicz R, Ercisli S and Jaafar HZ. (2014). Compositional studies and biological activities of some mash bean (Vigna mungo L. Hepper) cultivars commonly consumed in Pakistan. Biological Research, 47(1), 23–31.
Ahmad A, Husain A, Mujeeb M, Khan SA, Najmi AK, Siddique NA, Damanhouri ZA and Anwar F. (2013). A review on therapeutic potential of Nigella sativa: a miracle herb. Asian Pacific Journal of Tropical Biomedicine, 3(5), 337–52.
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