Identification of bacteria causing Vibriosis (Vibrio sp) on white snapper (Lates calcarifer) reared in the marine cultivation ponds

Salman Farisi; Endang Linirin Widiastuti; Suratman; Rakhmat Hadi Saputra; Mohammad Kanedi

doi:10.30574/gscbps.2021.14.1.0010

Authors

Salman Farisi Department of Biology, Faculty of Math and Sciences, University of Lampung, Bandar Lampung, Indonesia.
Endang Linirin Widiastuti Department of Biology, Faculty of Math and Sciences, University of Lampung, Bandar Lampung, Indonesia.
Suratman Department of Biology, Faculty of Math and Sciences, University of Lampung, Bandar Lampung, Indonesia.
Rakhmat Hadi Saputra Department of Biology, Faculty of Math and Sciences, University of Lampung, Bandar Lampung, Indonesia.
Mohammad Kanedi Department of Biology, Faculty of Math and Sciences, University of Lampung, Bandar Lampung, Indonesia.

DOI:

https://doi.org/10.30574/gscbps.2021.14.1.0010

Keywords:

Vibriosis, Vibrio bacteria, White snapper, Lates calcarifer

Abstract

Vibrio is a group of bacteria that causes Vibriosis in many aquatic biota cultivated in ponds. This research aims to determine the type of the Vibrio sp. which causes vibriosis disease in white snapper reared in the marine cultivation ponds at the Center for Marine Cultivation Fisheries (CMCF) Lampung, Indonesia. The research was conducted using investigative method by isolating Vibrio bacteria from the organ in white snapper, mud, and water. The bacterial isolates were indentified using biochemical test. The reseach parameters are TPC (Total Plate Count) Vibrio sp., water temperature, pH, salinity, DO, BOD and ammonia. The results showed there were three types of isolates that are suspected as the Vibriosis bacteria in white snapper fish namely Vibrio vulnificus, Vibrio alginolyticus and Vibrio parahaemolyticus. The three isolates obtained were known to be α-hemolysis. The enviromental quality parameters are as follows: water temperature of 29.8 °C, pH 7.8, salinity 33 psu, DO 4.4 mg / L, BOD 2.2 mg / L and ammonia 2.2 mg / L. The TPC-values of the bacteria Vibrio sp. isolated from the inlet, the main chambers, and the outlet of the pond are: <25 CFU/ ml, 7.6x104 CFU/ml, and 2.1x103 CFU/ml respectively. The levels of ammonia and calculation of TPC-values of Vibrio bacteria are exceeds the water quality standards for the fish farming.

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References

Felix F, Nugroho TT, Silalahi S,Octavia Y. Skrining Bakteri Vibrio sp Asli Indonesia sebagai Penyebab Penyakit Udang Berbasis Tehnik 16s Ribosomal DNA. Jurnal Ilmu dan Teknologi Kelautan Tropis. 2011; 3(2): 85-99.

Teng T, Liang L, Chen K, Xi B, Xie J, andXu P. Isolation, identification and phenotypic and molecular characterization of pathogenic Vibrio vulnificus isolated from Litopenaeus vannamei. Plos One. 2017; 12(10): 1-10.

Muliani, Suryati E, Tenriulo A, Tampangallo BR. Efektifitas Ekstrak Mangrove Osbornia octodanta pada Budidaya Udang Windu Penaeus monodon. Dalam A.Irianto (ed), Prosiding. Pengendalian Penyaki Pada Ikandan Udang Berbasis Imunisasi dan Biosecurity. Purwokerto. 2004; 60-66.

Krishnika A, Ramasamy P. Legenidium sp. Infection in the Larval Stages of the Freshwater Prawn Macrobrachium rosenbergii (de Man). Indian. J. Fish. 2014; 61(2): 90-96.

Taslihan A, Murdjani, Pubomartono C, Kusnendar E. Bakteri pathogen penyebab penyakit mulut merah pada ikan kerapu tikus. J Perikanan UGM. 2000; II(2):57-62.

Nelce MN, dan Setha B. Karakteristik Patogenitas Vibrio sp. Diisolasi Dari Lendir Sidat (Anguilla sp.). Jurnal kedokteran dan Kesehatan. Program Studi Pendidikan Dokter Universitas Pattimura. 2011; 4(1): 42-48.

Bergey DH, Boone DR. Bergey’s Manual of Systematic Bacteriology, Vol.3, Ed.2, 655, Springer Science-Business Media, New York. 2009.

CappucinoJG, Sherman N. Microbiology. A Laboratory Manual. Sixth Edition. Rockland Community College, New York. 2013; 491.

Sudheesh PS, Xu HS. Pathogenicity of Vibrio parahaemolyticus In Tiger Prawn Penaeus monodon Fabricus: Possible Role of Extracellular Proteases. Aquaculture. 2001; 196: 37-46.

Bintari NWD, Kawuri R, DalemAAGR. Identifikasi Bakteri Vibrio Penyebab Vibriosis Pada Larva Udang Galah (Macrobrachium rosen bergii (de Man)). Jurnal Biologi. 2016; 20(2): 53-63.

Melki D, Soedharma, Effendi H,Mustofa Z. BiopotensiTumbuhan Mangrove Untuk Pencegahan Penyakit Vibriosis Pada Udang Windu. Maspari J. 2011; 2: 39-47.

Sukenda, Widanarni, Haris E. Isolasi dan Karakterisasi Vibrio Pathogen pada Ikan Kerapu Macan (Epinephelus fuscoguttatus). Jurnal Akuakultur Indonesia. 2012; 11(1): 28-37.

Defoirdt T, Boon N, Bossier P, Verstraete W. Disruption of Bacterial Quorum Sensing: An Unexplored Strategy To Fight Infections In Aquaculture. Aquaqulture. 2004; 240:69-88.

Tatangindatu F, Kalesaran O, Rompas R. Studi Parameter Fisika Kimia Air Pada Areal Budidaya Ikan di Danau Tondano, Desa Paleloan, Kabupaten Minahasa. Budidaya Perairan. 2013;1(2): 8-19.