Anxiolytic and antipyretic properties of the root aqueous extract of Bombax costatum Pellegr. et Vuillet. (Bombacaceae) in mice: Implication of behavioural and neurochemical approaches

Authors

  • Ngassia Wanbara Department of Biological Sciences, Faculty of Science, University of Ngaoundere, P.O. Box 454, Ngaoundere, Cameroon.
  • Germain Sotoing Taiwe Department of Zoology and Animal Physiology, Faculty of Science, University of Buea, Cameroon, P.O. Box 63, Buea, Cameroon.
  • Jacqueline Stephanie Kameni Njapdounke Department of Biological Sciences, Faculty of Science, University of Ngaoundere, P.O. Box 454, Ngaoundere, Cameroon.
  • Neteydji Sidiki Department of Biological Sciences, Faculty of Science, University of Ngaoundere, P.O. Box 454, Ngaoundere, Cameroon.
  • Alexandre Michel Njan Nloga Department of Biological Sciences, Faculty of Science, University of Ngaoundere, P.O. Box 454, Ngaoundere, Cameroon.
  • Elisabeth Ngo Bum Department of Biological Sciences, Faculty of Science, University of Maroua, P.O. Box 814, Maroua, Cameroon.

DOI:

https://doi.org/10.30574/gscbps.2021.15.2.0133

Keywords:

Bombax costatum, Anxiolytic, GABAergic, Animal models, Traditional medicine

Abstract

Ethnopharmacological relevance: Bombax costatum Pellegr. et Vuillet. (Bombacaceaea) is a plant used in traditional medicine in Cameroon to treat memory impairment, anxiety, insomnia and agitation.

Aim of the study: The aqueous extract of Bombax costatum is evaluated for its anxiolytic like effect in mice using experimental models.

Materials and methods: The plant extract is administered orally to mice. They were tested one hour later in the stress-induced hyperthermia, hole board, and open field or elevated plus maze tests, respectively. Finally, the brain Gamma aminobutyric acid [GABA] content and GABA-T were quantified in Bombax costatum aqueous extract-treated mice at the end of elevated plus maze test.

Results: Bombax costatum aqueous extract showed anxiolytic activity. In stress-induced hyperthermia test, the plant extract significantly antagonised the increase of temperature. There is a significant reduction in the stress-induced hyperthermia from 1.13 ± 0.06℃ in the negative control group treated with distilled water to 0.26 ± 0.02℃ in the group of mice administered 100 mg/kg aqueous extract. In addition, Bombax costatum showed antipyretic activity by reducing the body temperature. In the elevated plus maze test, the aqueous extract increased the number of entries into, percentage of entries into, and percentage of time in open arms. It also reduced the percentage of entries and time in closed arms.

Conclusion: The obtained results suggested that Bombax costatum aqueous extracts possess anxiolytic-like and antipyretic activities in mice. This plant could be helpful in the treatment of anxiety and fever in traditional medicine in Cameroon.

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References

Martin A, et al. Validity of the brief patient health questionnaire mood scale (PHQ-9) in the general population. General hospital psychiatry. 2006; 28(1): 71-77.

Stein DJ, et al. Epidemiology of anxiety disorders: from surveys to nosology and back. Dialogues in clinical neuroscience. 2017; 19(2): 127.

Pitchaiah G et al. Pharmacological evaluation of alcoholic extract of stem bark of Erythrina variegate for anxiolytic and anticonvulsant activity in mice. Pharmacologyonline. 2008; 3: 934-937.

Le Naour, R., Anxiety disorders: diversity of theoretical approaches. INFORMATION PSYCHIATRIQUE. 2007; 83(9): 775.

World Health Organization. WHO traditional medicine strategy: 2014-2023. World Health Organization (2013).

Arbonnier M. Trees, shrubs and lianas in the dry zones of West Africa. Trees, shrubs and lianas in the dry zones of West Africa. 2000.

Taïwe GS, et al, Antipyretic and antinociceptive effects of Nauclea latifolia root decoction and possible mechanisms of action. Pharmaceutical biology. 2011; 49(1): 15-25.

Percie du Sert N, et al., The ARRIVE guidelines 2.0: Updated guidelines for reporting animal research. Journal of Cerebral Blood Flow & Metabolism. 2020; 40(9): 1769-1777.

Karp NA, et al., Applying the ARRIVE guidelines to an in vivo database. PLoS Biol. 2015; 13(5): e1002151.

Lecci A, et al., Pharmacological validation of a novel animal model of anticipatory anxiety in mice. Psychopharmacology; 1990; 101(2): 255-261.

Borsini F, et al, A model to measure anticipatory anxiety in mice? Psychopharmacology. 1989; 98(2): 207-211.

File SE, AG Wardill, Validity of head-dipping as a measure of exploration in a modified hole-board. Psychopharmacologia. 1975; 44(1): 53-59.

Takeda H, M Tsuji, T Matsumiya. Changes in head-dipping behavior in the hole-board test reflect the anxiogenic and/or anxiolytic state in mice. European journal of pharmacology. 1998; 350(1): 21-29.

Crawley JN. Exploratory behavior models of anxiety in mice. Neuroscience & Biobehavioral Reviews. 1985; 9(1): 37-44.

Taïwe G, et al, Antidepressant, myorelaxant and anti-anxiety-like effects of Nauclea latifolia smith (Rubiaceae) roots extract in murine models. International journal of pharmacology. 2010; 6(4): 364-371.

Brown C, F Burgess, VA Braithwaite. Heritable and experiential effects on boldness in a tropical poeciliid. Behavioral Ecology and Sociobiology. 2007; 62(2): 237-243.

Jenck F, et al, Orphanin FQ acts as an anxiolytic to attenuate behavioral responses to stress. Proceedings of the National Academy of Sciences. 1997; 94(26): 14854-14858.

Rodgers R, A Dalvi, Anxiety, defence and the elevated plus-maze. Neuroscience & Biobehavioral Reviews. 1997; 21(6): 801-810.

Lister RG. The use of a plus-maze to measure anxiety in the mouse. Psychopharmacology. 1987; 92(2): 180-185.

Bourin M, et al. Cyamemazine as an anxiolytic drug on the elevated plus maze and light/dark paradigm in mice. Behavioural brain research. 2001; 124(1): 87-95.

Ngo Bum EN, et al, Anticonvulsant, anxiolytic, and sedative properties of the roots of Nauclea latifolia Smith in mice. Epilepsy & Behavior. 2009; 15(4): 434-440.

Sutton I, MA Simmonds. Effects of acute and chronic pentobarbitone on the γ-aminobutyric acid system in rat brain. Biochemical pharmacology. 1974; 23(13): 1801-1808.

Nayak P, A Chatterjee. Effects of aluminium exposure on brain glutamate and GABA systems: an experimental study in rats. Food and chemical toxicology. 2001; 39(12): 1285-1289.

Taiwe GS, et al, Extracts of Feretia apodanthera Del. demonstrated anticonvulsant activities against seizures induced by chemicals and maximal electroshock. Epilepsy Research. 2016; 127: 30-39.

Borsini F, J Podhorna, D Marazziti Do animal models of anxiety predict anxiolytic-like effects of antidepressants? Psychopharmacology. 2002; 163(2): 121-141.

Augustsson H. Ethoexperimental studies of behaviour in wild and laboratory mice. Doctoral thesis

Swedish University of Agricultural Sciences, Uppsala (2004); 173-174.

Rodgers R et al., Animal models of anxiety: an ethological perspective. Brazilian journal of medical and biological research. 1997; 30: 289-304.

Belzung C, G Griebel. Measuring normal and pathological anxiety-like behaviour in mice: a review. Behavioural brain research. 2001; 125(1-2): 141-149.

Walf AA, CA Frye. The use of the elevated plus maze as an assay of anxiety-related behavior in rodents. Nature protocols. 2007; 2(2): 322-328.

Crawley J, FK Goodwin. Preliminary report of a simple animal behavior model for the anxiolytic effects of benzodiazepines. Pharmacology Biochemistry and Behavior. 1980; 13(2): 167-170.

Saad S, A El Masry, P Scott. Influence of certain anticonvulsants on the concentration of γ-aminobutyric acid in the cerebral hemispheres of mice. European journal of pharmacology. 1972; 17(3): 386-392.

Kamoun N et al., Long-lasting analgesic and neuroprotective action of the non-benzodiazepine anxiolytic etifoxine in a mouse model of neuropathic pain. Neuropharmacology. 2021; 182: 108-407.

Sherif FM, SS Ahmed. Basic aspects of GABA-transaminase in neuropsychiatric disorders. Clinical biochemistry. 1995; 28(2): 145-154.

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Published

2021-05-30

How to Cite

Wanbara, N. ., Taiwe, G. S. ., Njapdounke, J. S. K. ., Sidiki, N. ., Nloga, A. M. N. ., & Bum, E. N. . (2021). Anxiolytic and antipyretic properties of the root aqueous extract of Bombax costatum Pellegr. et Vuillet. (Bombacaceae) in mice: Implication of behavioural and neurochemical approaches. GSC Biological and Pharmaceutical Sciences, 15(2), 140–150. https://doi.org/10.30574/gscbps.2021.15.2.0133

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