Kalanchoe Pinnata aqueous extract has the potential to improve haematological profile and fatigue status of mice subjected to Free Swimming Endurance Test

Orelien Sylvain Mtopi  Bopda; Odine Perez  Maloba; Mbigah Pascal  Monju; Germain Sotoing  Taiwe; Théophile  Dimo

doi:10.30574/gscbps.2021.17.2.0327

Authors

Orelien Sylvain Mtopi Bopda Department of Zoology and Animal Physiology, Faculty of Science, University of Buea, P.O. Box 63, Buea (Cameroon).
Odine Perez Maloba Department of Zoology and Animal Physiology, Faculty of Science, University of Buea, P.O. Box 63, Buea (Cameroon).
Mbigah Pascal Monju Department of Zoology and Animal Physiology, Faculty of Science, University of Buea, P.O. Box 63, Buea (Cameroon).
Germain Sotoing Taiwe Department of Zoology and Animal Physiology, Faculty of Science, University of Buea, P.O. Box 63, Buea (Cameroon).
Théophile Dimo Department of Animal Biology and Physiology, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé (Cameroon).

DOI:

https://doi.org/10.30574/gscbps.2021.17.2.0327

Keywords:

Kalanchoe pinnata, Haematological parameters, Biochemical parameters, Exercise, Anti-fatigue, Mice

Abstract

Introduction: Kalanchoe pinnata extracts have been shown to possess beneficial cardiovascular effects, analgesic and myorelaxant activities. This study aimed at evaluating the haematological and anti-fatigue effects of its aqueous extract.

Materials and methods: This was an in vivo pharmacological experiment, carried out in the Laboratory of Zoology, University of Buea, from January to May 2018. A total of 48 mice were subjected to a 90mins/day-free endurance swimming test for 14 days. Two groups of mice received distilled water and 12% NaCl solution, respectively. Five groups received the plant extract (25, 50, 100 and 200 mg/kg/day) and 200mg/kg/day+NaCl. One positive control group received 5% Vitamin C (1.97mg/kg/day). All administrations were by gavage. Maximum swimming time, glycaemia, lactatemia, uremia, triglyceridemia, haematological indices, tissue glutathion peroxidase, malondialdehyde, glucose and creatine kinase-MB in the heart or gastrocnemius muscle were measured.

Results: K. pinnata (50 and 100mg/kg/day) induced a non-significant increase of the swimming duration, compared to neutral control. However, the 12%NaCl group recorded a significant (P<0.05) increase. In the blood, NaCl induced a decrease of platelets that was significantly reverted by the extract in the 200mg/kg+NaCl group. The extract prevented the increase of the level of CK-MB in NaCl group and decreased the serum triglycerides, glucose, urea nitrogen and Lactate levels. In the tissues, MDA and CK-MB levels significantly increased (P<0.001) in the negative control. These increases were significantly (P<0.001) prevented by K. pinnata (200mg/kg/day).

Conclusions: Significant biochemical variations support the hypothesis that K. pinnata could be used to delay exercise induced fatigue.

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