Green synthesis and biochemical characterization of silver nanoparticles by using Euphorbia umbellata leaf extract and analysis of antimicrobial activity against plant pathogens

Ajay Kumar Sahu; S.S Priyadarshini Harichandan; Prangya Paramita Acharya; Pinki Samal; Sakshi Gautam; Rani Kar Barsha; Kumar Kiran B

doi:10.30574/gscbps.2019.8.2.0137

Authors

Ajay Kumar Sahu Department of Microbiology, Bangalore University, Bangalore, Karnataka.
S.S Priyadarshini Harichandan Department of Biotechnology, Berhampur University, Odisha.
Prangya Paramita Acharya Department of Biotechnology, Sambalpur University, Odisha.
Pinki Samal Department of Microbiology, Sambalpur University, Odisha.
Sakshi Gautam Department of Biotechnology, Lovely Professional University, India.
Rani Kar Barsha Department of Biotechnology, Utkal University, Bhubaneswar. Odisha
Kumar Kiran B Department of Biotechnology, Reva University, Bangalore

DOI:

https://doi.org/10.30574/gscbps.2019.8.2.0137

Keywords:

Silver nanoparticles, Biochemical characterization, Antibacterial activity, TEM and SEM analysis, UV spectroscopy, XRD analysis

Abstract

The science of nanotechnology and nanoparticles is the manipulation of matter on atomic and molecular weight, the nanotechnology and nanomaterial’s refer to the particular technological goal which is precise manipulation of atom and molecules for used the fabrication process of microbial products and it is now concern to as molecular nanotechnology, it also known as the nanotechnology is science of designing, making and application of nano-structure and nanomaterials also used investigation of relationship various properties of materials with their nanometer dimensions. The exploitation of various plant materials for the biosynthesis of silver nano particles is considered a green technology. Because it does not involves any harmful chemicals. Nanotechnology field is one of the most attractive researches. The field of nanotechnology is applied to bio materials. Nanoparticles are generally considered as particles with a size up to 100 nm, that have completely new or improved properties as compared to the bulk material that they are collected based on particular characteristics such as size, distribution and morphology. Different groups of unorganized parts of the plants have been utilizing for the green synthesis of silver nanoparticles, in the present work the fresh leaves of euphorbia umbellate have been used for the synthesis of silver nanoparticles. Synthesis of AgNPs employing either microorganisms or plant extracts has emerged as an alternative approach. Silver nanoparticles is embedded with antibacterial properties because of its unique properties is considered in medical science, the main aim of work is green synthesis of silver nanoparticles using Euphorbia Umbellate leaf extract and its antibacterial activity, after the collection of sample, identification and extraction of Euphorbia Umbellate was performed the production of silver nanoparticles.

Metrics

Metrics Loading ...

References

Anker JN, Hall WP, Lyandres O, Shah NC, Zhao J and Van Duyne RP. (2008). Biosensing with plasmonic nanosensors. Nature Materials, 7, 442–453.

Byrappa K and Adschiri T. (2007). Hydrothermal technology for nanotechnology. Progress in Crystal Growth and Characterization of Materials, 53, 117–166.

Chandran SP, Chaudhary M, Pasricha R, Ahmad A and Sastry M. (2006). Synthesis of gold nanotriangles and silver nanoparticles using Aloe vera plant extract. Biotechnology Progress, 22, 577–583.

Cho K-H, Park J-E, Osaka T and Park S-G. (2005). The study of antimicrobial activity and preservative effects of nanosilver ingredient. Electrochimica Acta, 51, 956–960.

Chudasama B, Vala AK, Andhariya N, Mehta R and Upadhyay R. (2010). Highly bacterial resistant silver nanoparticles: synthesis and antibacterial activities. Journal of Nanoparticle Research, 12, 1677–1685.

Cui L, Chen P, Chen S, Yuan Z, Yu C, Ren B and Zhang K. (2013). In situ study of the antibacterial activity and mechanism of action of silver nanoparticles by surface-enhanced Raman spectroscopy. Analytical Chemistry, 85, 5436–5443.

Emaga TH, Robert C, Ronkart SN, Wathelet B and Paquot M. (2008). Dietary fibre components and pectin chemical features of peels during ripening in banana and plantain varieties. Bioresource Technology, 99, 4346–4354.

Feng Q, Wu J, Chen G, Cui F, Kim T and Kim J. (2000). A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. Journal of Biomedical Materials Research, 52, 662–668.

Griffin SG, Wyllie SG, Markham JL and Leach DN. (1999). The role of structure and molecular properties of terpenoids in determining their antimicrobial activity. Flavour and Fragrance Journal, 14, 322–332.

Hajipour MJ, Fromm KM, Ashkarran AA, De Aberasturi DJ, De Larramendi IR, Rojo T, Serpooshan V, Parak WJ and Mahmoudi M. (2012). Antibacterial properties of nanoparticles. Trends in Biotechnology, 30, 499–511.

Lee G-J, Shin S-I, Kim Y-C and Oh SG. (2004). Preparation of silver nanorods through the control of temperature and pH of reaction medium. Materials Chemistry and Physics, 84, 197–204.

Lee K-S and El-Sayed MA. (2006). Gold and silver nanoparticles in sensing and imaging: sensitivity of plasmon response to size, shape, and metal composition. The Journal of Physical Chemistry B. 110, 19220–19225.

Liu J, Sonshine DA, Shervani S and Hurt RH. (2010). Controlled release of biologically active silver from nanosilver surfaces. ACS Nano, 4, 6903–6913.

Liu Z, Wang Y, Zu Y, Fu Y, Li N, Guo N, Liu R and Zhang Y. (2014).Synthesis of polyethylenimine (PEI) functionalized silver nanoparticles by a hydrothermal method and their antibacterial activity study. Materials Science and Engineering: C, 42, 31–37.

Liu Z, Xing Z, Zu Y, Tan S, Zhao L, Zhou Z and Sun T. (2012). Synthesis and characterization of L-histidine capped silver nanoparticles. Materials Science and Engineering: C, 32, 811–816.

Livermore DM. (2002). Multiple mechanisms of antimicrobial resistance in Pseudomonas aeruginosa: our worst nightmare? Clinical Infectious Diseases, 34(5), 634–640.

Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, Ramirez JT and Yacaman MJ. (2005). The bactericidal effect of silver nanoparticles. Nanotechnology. 16, 2346–2353.

Nabikhan A, Kandasamy K, Raj A and Alikunhi NM. (2010). Synthesis of antimicrobial silver nanoparticles by callus and leaf extracts from saltmarsh plant, Sesuvium portulacastrum L. Colloids and Surfaces B: Biointerfaces. 79, 488–493.

Otto M. (2009). Staphylococcus epidermidis–the “accidental” pathogen. Nature Reviews Microbiology. 7(8), 555–567.

Pal S, Tak YK and Song JM. (2007). Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the gram-negative bacterium Escherichia coli. Applied and Environmental Microbiology, 73, 1712–1720.

Pradhan N, Pal A and Pal T. (2002).Silver nanoparticle catalyzed reduction of aromatic nitro compounds. Colloids and Surfaces A: Physicochemical and Engineering Aspect, 196, 247–257.

Rai M, Deshmukh S, Ingle A and Gade A. (2012).Silver nanoparticles: the powerful nanoweapon against multidrug resistant bacteria. Journal of Applied Microbiology, 112, 841–852.

Rai M, Yadav A and Gade A. (2009). Silver nanoparticles as a new generation of antimicrobials. Biotechnology Advances, 27, 76–83.

Rajan R, Chandran K, Harper SL, Yun S-I and Kalaichelvan PT. (2015).Plant extract synthesized silver nanoparticles: an ongoing source of novel biocompatible materials. Industrial Crops and Products, 70, 356–373.

Reynolds T and Dweck A. (1999). Aloe vera leaf gel: a review update. Journal of Ethnopharmacology, 68, 3–37.

Sahu PK, Giri DD, Singh R, Pandey P, Gupta S, Shrivastava AK, Kumar A and Pandey KD. (2013).Therapeutic and medicinal uses of Aloe vera: a review. Pharmacology & Pharmacy, 4, 599–610.

Sharma VK, Yngard RA and Lin Y. (2009). Silver nanoparticles: green synthesis and their antimicrobial activities. Advances in Colloid and Interface Science, 145, 83–96.

Sun Q, Cai X, Li J, Zheng M, Chen Z and Yu C-P. (2014). Green synthesis of silver nanoparticles using tea leaf extract and evaluation of their stability and antibacterial activity. Colloids and Surfaces A: Physicochemical and Engineering Aspects. 444, 226–231.

Sun Y, Mayers B and Xia Y. (2003).Transformation of silver nanospheres into nanobelts and triangular nanoplates through a thermal process. Nano Letters, 3, 675–679.

Sun Y and Xia Y. (2002). Shape-controlled synthesis of gold and silver nanoparticles. Science, 298, 2176–2179.

Taleb A, Petit C and Pileni M. (1997). Synthesis of highly monodisperse silver nanoparticles from AOT reverse micelles: a way to 2D and 3D self-organization. Chemistry of Materials, 9, 950–959.

Tamboli DP and Lee DS. (2014). Mechanistic antimicrobial approach of extracellularly synthesized silver nanoparticles against gram positive and gram negative bacteria. Journal of Hazardous Materials, 260, 878–884.

Vazquez B, Avila G, Segura D and Escalante B. (1996). Antiinflammatory activity of extracts from Aloe vera gel. Journal of Ethnopharmacology, 55, 69–75.

Yousefzadi M, Rahimi Z and Ghafori V. (2014). The green synthesis, characterization and antimicrobial activities of silver nanoparticles synthesized from green alga Enteromorpha flexuosa (Wulfen) J. Agardh. Materials Letters, 137, 1–4.

Zhang Y, Cheng X, Zhang Y, Xue X and Fu Y. (2013). Biosynthesis of silver nanoparticles at room temperature using aqueous aloe leaf extract and antibacterial properties. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 423, 63–68.

Zhang Y, Yang D, Kong Y, Wang X, Pandoli O and Gao G. (2010). Synergetic antibacterial effects of silver nanoparticles@ Aloe vera prepared via a green method. Nano Biomedicine and Engineering, 2, 252–257.

Ranganathan R, Vijayalakshmi R and Parameswari P. (2012). Ethnomedicinal survey of Jawadhu hills in Tamil Nadu, Asian J Pharm Clinical Res, 5(2).

Johnsy G, Davidson S and Kaviyarasan V. (2012). Indigenous knowledge of medicinal plants used for the treatment of skin diseases by the kaani tribe of Kanyakumari district, Int Pharm Pharmaceut Sci, 4, 1.

Farnsworth NR, Akerele O, Bingel AS, Soejarto DD and Guo Z. Medicinal plants in therapy, Bull WHO, 63(6), 965–981.