Gas chromatography and mass spectroscopy analysis and phytochemical characterization of Aegle marmelos (Bael) leaf, Stem and its screening of antimicrobial activity

Ajay Kumar Sahu; Barsha Rani Kar; Kiran Deepthi; S. Karthika Pallath; Simran J Dakni; Pinki Samal; Nagilla S Niharika; T Swetha; T Sneha; Anthony Surya Prakash A.; G Varshini Raju H.

doi:10.30574/gscbps.2019.8.3.0159

Authors

Ajay Kumar Sahu Department of Microbiology, Bangalore University, Bangalore, India.
Barsha Rani Kar Department of Biotechnology, AMIT College, Utkal University, Bhubaneswar, Odisha, India.
Kiran Deepthi Department of Microbiology, Bangalore University, Bangalore, India.
S. Karthika Pallath Department of Bioscience, Mahatma Gandhi University, India.
Simran J Dakni Department of Biotechnology, Bangalore University, Bangalore, India.
Pinki Samal Department of Biotechnology, Bangalore University, Bangalore, India.
Nagilla S Niharika Department of Microbiology, Bangalore University, Bangalore, India.
T Swetha Department of Microbiology, Bangalore University, Bangalore, India.
T Sneha Department of Microbiology, Bangalore University, Bangalore, India.
Anthony Surya Prakash A. Department of Microbiology, Bangalore University, Bangalore, India.
G Varshini Raju H. Department of Microbiology, Bangalore University, Bangalore, India.

DOI:

https://doi.org/10.30574/gscbps.2019.8.3.0159

Keywords:

Gas chromatography and mass spectroscopy, Phytochemical analysis, Aegle marmelos leaf, Antimicrobial activity

Abstract

Phytochemical screening tests was conducted for five plant species and found that extract contains a variety of Phytochemical like saponins, tannins, flavonoids, terpenoids, glycosides and reducing sugars and among which there is higher level of precipitation for phenol and flavonoids. As they are essential source of antimicrobial agents against pathogen, their extract were tested for its antimicrobial activity by well diffusion method using Nutrient agar against human pathogenic bacteria like Staphylococcus aureus, Escherichia coli. This study provided evidence to confirm the presence of various medicinally important bioactive compounds or Phytochemical that has got biological importance and it justifies their use in the traditional medicines for the treatment of different diseases and this findings suggest that the selected plant extracts possesses antimicrobial properties that could be used for biological control of bacterial cultures and this bioactive compounds serve as a source of antimicrobial agents against human pathogens. Medicinal plants have bioactive compounds which are used for curing of various human diseases and also play an important role in healing. Phytochemical have two categories i.e., primary and secondary constituent. The phytochemical analysis of the plants is very important commercially and has great interest in pharmaceutical companies for the production of the new drugs for curing of various diseases. this GC-MS has been used as standard protocol for a foreign substance identification because of its used to identify the particular specific test results which is indicates or identifies the presences of that particular substance. The aqueous leaf extract were used for to identify and the phytochemical analysis used to find out the phytochemical constituents presents at the taken plants. Plant showed that the alkaloids, trepenoids, phenol and tannins, reducing sugar, saponin, proteins, anthocyanin, coumarin and glycosides were found to be presents in the given plants. Gas chromatography with mass spectroscopy detection is the state of the art methods for detection and identification of unknown compound, it is also not infallible and many compounds are difficulty with their accuracy certainty.

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